The effect of genomic position on reversion of a lac frameshift mutation (lacIZ33) during non-lethal selection (adaptive mutation)

E. Susan Slechta, Jennifer Harold, Dan I. Andersson, John R. Roth

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

In a system described by Cairns and Foster, starvation of a particular leaky lac mutant (lacIZ33) in the presence of lactose appears to direct mutation in non-growing cells to sites that allow growth (adaptive mutation). This behaviour requires that the lac operon be located on an F′ plasmid. This position effect was investigated by placing the mutant lac operon at many sites in the genome of Salmonella enterica (Typhimurium; LT2) and testing reversion behaviour. Genomic position did not affect reversion during non-selective growth. When lac was at any of 550 chromosomal sites, starvation caused little or no enhancement of reversion. In the 28 strains with the lac on Salmonella's conjugative plasmid (pSLT), selection enhanced reversion strongly, just as seen for strains with lac on an F′ plasmid. In 46 strains, the lac operon was inserted within a small chromosomal duplication, and selection stimulated RecA-dependent partial reversion by simple amplification (about 8×) of the mutant lac region. The position of lac on a conjugative plasmid is important to reversion because it allows more frequent gene duplication and amplification. These events are central to growth and reversion under selection because they increase the number of replicating lac alleles within each developing revertant clone.

Original languageEnglish (US)
Pages (from-to)1017-1032
Number of pages16
JournalMolecular Microbiology
Volume44
Issue number4
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Frameshift Mutation
Lac Operon
F Factor
Starvation
Mutation
Plasmids
Growth
Chromosome Duplication
Gene Duplication
Salmonella enterica
Gene Amplification
Salmonella typhimurium
Lactose
Salmonella
Clone Cells
Alleles
Genome

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

The effect of genomic position on reversion of a lac frameshift mutation (lacIZ33) during non-lethal selection (adaptive mutation). / Slechta, E. Susan; Harold, Jennifer; Andersson, Dan I.; Roth, John R.

In: Molecular Microbiology, Vol. 44, No. 4, 2002, p. 1017-1032.

Research output: Contribution to journalArticle

Slechta, E. Susan ; Harold, Jennifer ; Andersson, Dan I. ; Roth, John R. / The effect of genomic position on reversion of a lac frameshift mutation (lacIZ33) during non-lethal selection (adaptive mutation). In: Molecular Microbiology. 2002 ; Vol. 44, No. 4. pp. 1017-1032.
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