The effect of euthanasia technique on vascular arachidonic acid metabolism and vascular and intestinal smooth muscle contractility

M. M. Butler, Stephen M Griffey, F. J. Clubb, L. W. Gerrity, W. B. Campbell

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

This study was designed to determine the effects that specific euthanasia methods have on vascular arachidonic acid metabolism and vascular and intestinal smooth muscle contractility. Rats were euthanatized by decapitation (DC), pentobarbital overdose (PB), or anesthesia with CO2, methoxyflurane or ether followed by DC (CO2-DC, Met-DC, Ether-DC, respectively). Rabbits were killed by a similar protocol, but CO2 overexposure replaced Ether-DC. The rat and rabbit aortas produced mainly 6-keto PGF(1α), the prostacyclin metabolite, and lesser amounts of PGE2. No qualitative differences were seen in arachidonate metabolites. However, aortic tissues from rabbits and rats killed by Met-DC produced more prostacyclin. In contrast, aorta from rabbits euthanatized by CO2-DC produced less prostacyclin than controls, whereas aorta from rats killed in the same way yielded greater amounts of prostacyclin. Aortic tissue from rabbits killed by Met-DC and CO2-OD was less responsive to acetylcholine (ACH). Intestinal contractility to ACH was increased in rabbits when Met-DC was used as the method of euthanasia, while colon from rats sacrificed by Met-DC showed decreased responsiveness to ACH. Colon from rats killed by intraperitoneal PB exhibited altered contractility to ACH and norepinephrine. The results of this study show that methoxyflurane, carbon dioxide (rabbit) and pentobarbital (rat) alter the vascular synthesis of prostacyclin and smooth muscle contractility. We conclude that the method of euthanasia affects certain physiologic parameters and careful consideration should be given to the selection of a particular euthanasia technique.

Original languageEnglish (US)
Pages (from-to)277-283
Number of pages7
JournalLaboratory Animal Science
Volume40
Issue number3
StatePublished - 1990
Externally publishedYes

Fingerprint

Decapitation
muscle strength
decapitation
Euthanasia
euthanasia
arachidonic acid
Vascular Smooth Muscle
blood vessels
Arachidonic Acid
Metabolism
smooth muscle
Blood Vessels
Muscle
Rats
Epoprostenol
prostacyclin
metabolism
Acetylcholine
rabbits
Pentobarbital

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

The effect of euthanasia technique on vascular arachidonic acid metabolism and vascular and intestinal smooth muscle contractility. / Butler, M. M.; Griffey, Stephen M; Clubb, F. J.; Gerrity, L. W.; Campbell, W. B.

In: Laboratory Animal Science, Vol. 40, No. 3, 1990, p. 277-283.

Research output: Contribution to journalArticle

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