The effect of age on manganese uptake and retention from milk and infant formulas in rats

Carl L Keen, J. G. Bell, B. Lonnerdal

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Manganese nutrition of the neonate is poorly understood, due in part to a paucity of information on the amount and availability of manganese in infant foods. We have developed a suckling pup model to assess the uptake of manganese from fluid diets by using extrinsic labeling. Human milk, cow milk, and infant formulas were fed by intubation to fasted rat pups and adults. Rats were killed after varying time periods, and tissues were removed and counted. A period of 6 h was found to be adequate to allow for stomach emptying while limiting tissue redistribution; 24 h was found to reflect pup manganese retention. From human milk, manganese retention was highest (≥80%) in pups ≤15 days of age; in older pups average retention decreased to 40%. Using d 14 pups to assess relative Mn uptake from diets, whole-body Mn uptake was highest from cow milk (~89%); uptake from human and cow milk formula was similar (~80%) whereas it was lower from soy formula (~60%). These findings suggest that bioavailability of Mn from infant diets is very high during the suckling period. Since most formulas contain considerably more manganese than is found in human milk, Mn deficiency may be less of a concern than possible toxicity from formulas.

Original languageEnglish (US)
Pages (from-to)395-402
Number of pages8
JournalJournal of Nutrition
Volume116
Issue number3
StatePublished - 1986

Fingerprint

Infant Formula
infant formulas
Manganese
pups
manganese
Milk
Human Milk
breast milk
uptake mechanisms
milk
rats
Diet
suckling
diet
Infant Food
gastric emptying
infant foods
Intubation
Biological Availability
bioavailability

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)

Cite this

The effect of age on manganese uptake and retention from milk and infant formulas in rats. / Keen, Carl L; Bell, J. G.; Lonnerdal, B.

In: Journal of Nutrition, Vol. 116, No. 3, 1986, p. 395-402.

Research output: Contribution to journalArticle

Keen, CL, Bell, JG & Lonnerdal, B 1986, 'The effect of age on manganese uptake and retention from milk and infant formulas in rats', Journal of Nutrition, vol. 116, no. 3, pp. 395-402.
Keen, Carl L ; Bell, J. G. ; Lonnerdal, B. / The effect of age on manganese uptake and retention from milk and infant formulas in rats. In: Journal of Nutrition. 1986 ; Vol. 116, No. 3. pp. 395-402.
@article{83058a914eff4621871057a004246f8b,
title = "The effect of age on manganese uptake and retention from milk and infant formulas in rats",
abstract = "Manganese nutrition of the neonate is poorly understood, due in part to a paucity of information on the amount and availability of manganese in infant foods. We have developed a suckling pup model to assess the uptake of manganese from fluid diets by using extrinsic labeling. Human milk, cow milk, and infant formulas were fed by intubation to fasted rat pups and adults. Rats were killed after varying time periods, and tissues were removed and counted. A period of 6 h was found to be adequate to allow for stomach emptying while limiting tissue redistribution; 24 h was found to reflect pup manganese retention. From human milk, manganese retention was highest (≥80{\%}) in pups ≤15 days of age; in older pups average retention decreased to 40{\%}. Using d 14 pups to assess relative Mn uptake from diets, whole-body Mn uptake was highest from cow milk (~89{\%}); uptake from human and cow milk formula was similar (~80{\%}) whereas it was lower from soy formula (~60{\%}). These findings suggest that bioavailability of Mn from infant diets is very high during the suckling period. Since most formulas contain considerably more manganese than is found in human milk, Mn deficiency may be less of a concern than possible toxicity from formulas.",
author = "Keen, {Carl L} and Bell, {J. G.} and B. Lonnerdal",
year = "1986",
language = "English (US)",
volume = "116",
pages = "395--402",
journal = "Journal of Nutrition",
issn = "0022-3166",
publisher = "American Society for Nutrition",
number = "3",

}

TY - JOUR

T1 - The effect of age on manganese uptake and retention from milk and infant formulas in rats

AU - Keen, Carl L

AU - Bell, J. G.

AU - Lonnerdal, B.

PY - 1986

Y1 - 1986

N2 - Manganese nutrition of the neonate is poorly understood, due in part to a paucity of information on the amount and availability of manganese in infant foods. We have developed a suckling pup model to assess the uptake of manganese from fluid diets by using extrinsic labeling. Human milk, cow milk, and infant formulas were fed by intubation to fasted rat pups and adults. Rats were killed after varying time periods, and tissues were removed and counted. A period of 6 h was found to be adequate to allow for stomach emptying while limiting tissue redistribution; 24 h was found to reflect pup manganese retention. From human milk, manganese retention was highest (≥80%) in pups ≤15 days of age; in older pups average retention decreased to 40%. Using d 14 pups to assess relative Mn uptake from diets, whole-body Mn uptake was highest from cow milk (~89%); uptake from human and cow milk formula was similar (~80%) whereas it was lower from soy formula (~60%). These findings suggest that bioavailability of Mn from infant diets is very high during the suckling period. Since most formulas contain considerably more manganese than is found in human milk, Mn deficiency may be less of a concern than possible toxicity from formulas.

AB - Manganese nutrition of the neonate is poorly understood, due in part to a paucity of information on the amount and availability of manganese in infant foods. We have developed a suckling pup model to assess the uptake of manganese from fluid diets by using extrinsic labeling. Human milk, cow milk, and infant formulas were fed by intubation to fasted rat pups and adults. Rats were killed after varying time periods, and tissues were removed and counted. A period of 6 h was found to be adequate to allow for stomach emptying while limiting tissue redistribution; 24 h was found to reflect pup manganese retention. From human milk, manganese retention was highest (≥80%) in pups ≤15 days of age; in older pups average retention decreased to 40%. Using d 14 pups to assess relative Mn uptake from diets, whole-body Mn uptake was highest from cow milk (~89%); uptake from human and cow milk formula was similar (~80%) whereas it was lower from soy formula (~60%). These findings suggest that bioavailability of Mn from infant diets is very high during the suckling period. Since most formulas contain considerably more manganese than is found in human milk, Mn deficiency may be less of a concern than possible toxicity from formulas.

UR - http://www.scopus.com/inward/record.url?scp=0022651524&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022651524&partnerID=8YFLogxK

M3 - Article

C2 - 3950766

AN - SCOPUS:0022651524

VL - 116

SP - 395

EP - 402

JO - Journal of Nutrition

JF - Journal of Nutrition

SN - 0022-3166

IS - 3

ER -