The effect of adipose-derived stem cells on augmentation ileocystoplasty: A pilot study

Ahmed M. Harraz, Guiting Lin, Lia Banie, Guifang Wang, Alan W. Shindel, Yun Ching Huang, Thomas M. Fandel, Maurice Garcia, Tom F. Lue, Ching Shwun Lin

Research output: Contribution to journalArticle

Abstract

Objectives: Incorporation of intestinal tissue into urinary tract elicits many metabolic and mechanical complications due to anatomical and physiological differences. Adipose-derived stem cells (ADSCs) improve vascularity and functional outcomes by a paracrine mechanism. In a pilot study we investigated whether ADSCs can survive in the augmented bladder and improve its function. Materials and methods: Autologous ADSCs were harvested from rat paragonadal fat and cultured before injection into a rat model of augmentation ileocystoplasty (study group). Control augmented bladders were injected with cell-free saline. Eight weeks later, rats underwent abdominal ultrasonography for upper tract changes and were examined by conscious cystometry to determine bladder function. After extirpation, augmented bladders were examined using Masson trichrome staining for connective tissue and muscle content, immunohistochemistry for α-smooth muscle actin, and rat endothelial cell antigen staining for endothelial cells. Changes in the extracellular matrix were assessed by determining the elastin content. ADSCs were labelled and tracked by 5-ethynyl-2-deoxyuridine nuclear staining. Results: Abdominal ultrasonography showed better preservation of upper tract function in the ADSC group than in the saline-treated group (P = 0.007). After 2 months there were no differences in the variables assessed by conscious cystometry between the ADSC and saline-treated groups. However, the bladder weight was significantly greater in the ADSC-treated group. On immunohistochemistry, the implanted ADSCs survived up to 8 weeks but did not transdifferentiate into smooth muscle or endothelial cells. Conclusion: These results suggested a potential role of ADSCs in modifying the intestinal segment in augmented bladders; this role has to be further elucidated.

Original languageEnglish (US)
Pages (from-to)139-145
Number of pages7
JournalArab Journal of Urology
Volume9
Issue number2
DOIs
StatePublished - Jun 2011

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Stem Cells
Urinary Bladder
Endothelial Cells
Staining and Labeling
Ultrasonography
Immunohistochemistry
Elastin
Urinary Tract
Connective Tissue
Smooth Muscle Myocytes
Extracellular Matrix
Fats
Antigens
Weights and Measures
Muscles
Injections

Keywords

  • 5-ethynyl-2-deoxyuridine
  • Conscious cystometry
  • Derived stem cells
  • Ileocystoplasty

ASJC Scopus subject areas

  • Urology

Cite this

The effect of adipose-derived stem cells on augmentation ileocystoplasty : A pilot study. / Harraz, Ahmed M.; Lin, Guiting; Banie, Lia; Wang, Guifang; Shindel, Alan W.; Huang, Yun Ching; Fandel, Thomas M.; Garcia, Maurice; Lue, Tom F.; Lin, Ching Shwun.

In: Arab Journal of Urology, Vol. 9, No. 2, 06.2011, p. 139-145.

Research output: Contribution to journalArticle

Harraz, AM, Lin, G, Banie, L, Wang, G, Shindel, AW, Huang, YC, Fandel, TM, Garcia, M, Lue, TF & Lin, CS 2011, 'The effect of adipose-derived stem cells on augmentation ileocystoplasty: A pilot study', Arab Journal of Urology, vol. 9, no. 2, pp. 139-145. https://doi.org/10.1016/j.aju.2011.07.002
Harraz, Ahmed M. ; Lin, Guiting ; Banie, Lia ; Wang, Guifang ; Shindel, Alan W. ; Huang, Yun Ching ; Fandel, Thomas M. ; Garcia, Maurice ; Lue, Tom F. ; Lin, Ching Shwun. / The effect of adipose-derived stem cells on augmentation ileocystoplasty : A pilot study. In: Arab Journal of Urology. 2011 ; Vol. 9, No. 2. pp. 139-145.
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abstract = "Objectives: Incorporation of intestinal tissue into urinary tract elicits many metabolic and mechanical complications due to anatomical and physiological differences. Adipose-derived stem cells (ADSCs) improve vascularity and functional outcomes by a paracrine mechanism. In a pilot study we investigated whether ADSCs can survive in the augmented bladder and improve its function. Materials and methods: Autologous ADSCs were harvested from rat paragonadal fat and cultured before injection into a rat model of augmentation ileocystoplasty (study group). Control augmented bladders were injected with cell-free saline. Eight weeks later, rats underwent abdominal ultrasonography for upper tract changes and were examined by conscious cystometry to determine bladder function. After extirpation, augmented bladders were examined using Masson trichrome staining for connective tissue and muscle content, immunohistochemistry for α-smooth muscle actin, and rat endothelial cell antigen staining for endothelial cells. Changes in the extracellular matrix were assessed by determining the elastin content. ADSCs were labelled and tracked by 5-ethynyl-2-deoxyuridine nuclear staining. Results: Abdominal ultrasonography showed better preservation of upper tract function in the ADSC group than in the saline-treated group (P = 0.007). After 2 months there were no differences in the variables assessed by conscious cystometry between the ADSC and saline-treated groups. However, the bladder weight was significantly greater in the ADSC-treated group. On immunohistochemistry, the implanted ADSCs survived up to 8 weeks but did not transdifferentiate into smooth muscle or endothelial cells. Conclusion: These results suggested a potential role of ADSCs in modifying the intestinal segment in augmented bladders; this role has to be further elucidated.",
keywords = "5-ethynyl-2-deoxyuridine, Conscious cystometry, Derived stem cells, Ileocystoplasty",
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T2 - A pilot study

AU - Harraz, Ahmed M.

AU - Lin, Guiting

AU - Banie, Lia

AU - Wang, Guifang

AU - Shindel, Alan W.

AU - Huang, Yun Ching

AU - Fandel, Thomas M.

AU - Garcia, Maurice

AU - Lue, Tom F.

AU - Lin, Ching Shwun

PY - 2011/6

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N2 - Objectives: Incorporation of intestinal tissue into urinary tract elicits many metabolic and mechanical complications due to anatomical and physiological differences. Adipose-derived stem cells (ADSCs) improve vascularity and functional outcomes by a paracrine mechanism. In a pilot study we investigated whether ADSCs can survive in the augmented bladder and improve its function. Materials and methods: Autologous ADSCs were harvested from rat paragonadal fat and cultured before injection into a rat model of augmentation ileocystoplasty (study group). Control augmented bladders were injected with cell-free saline. Eight weeks later, rats underwent abdominal ultrasonography for upper tract changes and were examined by conscious cystometry to determine bladder function. After extirpation, augmented bladders were examined using Masson trichrome staining for connective tissue and muscle content, immunohistochemistry for α-smooth muscle actin, and rat endothelial cell antigen staining for endothelial cells. Changes in the extracellular matrix were assessed by determining the elastin content. ADSCs were labelled and tracked by 5-ethynyl-2-deoxyuridine nuclear staining. Results: Abdominal ultrasonography showed better preservation of upper tract function in the ADSC group than in the saline-treated group (P = 0.007). After 2 months there were no differences in the variables assessed by conscious cystometry between the ADSC and saline-treated groups. However, the bladder weight was significantly greater in the ADSC-treated group. On immunohistochemistry, the implanted ADSCs survived up to 8 weeks but did not transdifferentiate into smooth muscle or endothelial cells. Conclusion: These results suggested a potential role of ADSCs in modifying the intestinal segment in augmented bladders; this role has to be further elucidated.

AB - Objectives: Incorporation of intestinal tissue into urinary tract elicits many metabolic and mechanical complications due to anatomical and physiological differences. Adipose-derived stem cells (ADSCs) improve vascularity and functional outcomes by a paracrine mechanism. In a pilot study we investigated whether ADSCs can survive in the augmented bladder and improve its function. Materials and methods: Autologous ADSCs were harvested from rat paragonadal fat and cultured before injection into a rat model of augmentation ileocystoplasty (study group). Control augmented bladders were injected with cell-free saline. Eight weeks later, rats underwent abdominal ultrasonography for upper tract changes and were examined by conscious cystometry to determine bladder function. After extirpation, augmented bladders were examined using Masson trichrome staining for connective tissue and muscle content, immunohistochemistry for α-smooth muscle actin, and rat endothelial cell antigen staining for endothelial cells. Changes in the extracellular matrix were assessed by determining the elastin content. ADSCs were labelled and tracked by 5-ethynyl-2-deoxyuridine nuclear staining. Results: Abdominal ultrasonography showed better preservation of upper tract function in the ADSC group than in the saline-treated group (P = 0.007). After 2 months there were no differences in the variables assessed by conscious cystometry between the ADSC and saline-treated groups. However, the bladder weight was significantly greater in the ADSC-treated group. On immunohistochemistry, the implanted ADSCs survived up to 8 weeks but did not transdifferentiate into smooth muscle or endothelial cells. Conclusion: These results suggested a potential role of ADSCs in modifying the intestinal segment in augmented bladders; this role has to be further elucidated.

KW - 5-ethynyl-2-deoxyuridine

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KW - Derived stem cells

KW - Ileocystoplasty

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