The dynamics of the T-cell antitumor response: Chemokine-secreting dendritic cells can prime tumor-reactive T cells extranodally

C. J. Kirk, D. Hartigan-O'Connor, Dennis Hartigan-O'Connor

Research output: Contribution to journalArticle

103 Scopus citations

Abstract

Direct administration of dendritic cells (DCs) genetically modified to express secondary lymphoid tissue chemokine (SLC) into growing B16 melanoma could result in a substantial, sustained influx of T cells within the mass with only a transient increase in T-cell numbers in the draining lymph node (DLN). DCs were retained at the tumor site with only a very small percentage trafficking to the DLN. The T cells infiltrating the tumor mass expressed the activation marker CD25 within 24 h and developed IFN-γ-secreting function within 7 days as tumor growth was inhibited. Similar results were obtained in lymphotoxin α-/- mice, which lacked peripheral lymph nodes. Our data demonstrate that effective T-cell priming can occur extranodally and result in measurable antitumor effects in vivo.

Original languageEnglish (US)
Pages (from-to)8794-8802
Number of pages9
JournalCancer Research
Volume61
Issue number24
StatePublished - Dec 15 2001
Externally publishedYes

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ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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