The dynamic envelope of a fusion Class II virus

Prefusion stages of Semliki Forest virus revealed by electron cryomicroscopy

Shang Rung Wu, Lars Haag, Lena Hammar, Bomu Wu, Henrik Garoff, Li Xing, Kazuyoshi Murata, R. Holland Cheng

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Semliki Forest virus is among the prototypes for Class II virus fusion and targets the endosomal membrane. Fusion protein E1 and its envelope companion E2 are both anchored in the viral membrane and form an external shell with protruding spikes. In acid environments, mimicking the early endosomal milieu, surface epitopes in the virus rearrange along with exposure of the fusion loop. To visualize this transformation into a fusogenic stage, we determined the structure of the virus at gradually lower pH values. The results show that while the fusion loop is available for external interaction and the shell and stalk domains of the spike begin to deteriorate, the E1 and E2 remain in close contact in the spike head. This unexpected observation points to E1 and E2 cooperation beyond the fusion loop exposure stage and implies a more prominent role for E2 in guiding membrane close encounter than has been earlier anticipated.

Original languageEnglish (US)
Pages (from-to)6752-6762
Number of pages11
JournalJournal of Biological Chemistry
Volume282
Issue number9
DOIs
StatePublished - Mar 2 2007

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Semliki forest virus
Cryoelectron Microscopy
Viruses
Fusion reactions
Membranes
Electrons
Viral Structures
Epitopes
Head
Observation
Acids
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

The dynamic envelope of a fusion Class II virus : Prefusion stages of Semliki Forest virus revealed by electron cryomicroscopy. / Wu, Shang Rung; Haag, Lars; Hammar, Lena; Wu, Bomu; Garoff, Henrik; Xing, Li; Murata, Kazuyoshi; Holland Cheng, R.

In: Journal of Biological Chemistry, Vol. 282, No. 9, 02.03.2007, p. 6752-6762.

Research output: Contribution to journalArticle

Wu, Shang Rung ; Haag, Lars ; Hammar, Lena ; Wu, Bomu ; Garoff, Henrik ; Xing, Li ; Murata, Kazuyoshi ; Holland Cheng, R. / The dynamic envelope of a fusion Class II virus : Prefusion stages of Semliki Forest virus revealed by electron cryomicroscopy. In: Journal of Biological Chemistry. 2007 ; Vol. 282, No. 9. pp. 6752-6762.
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