The Drosophila 74EF early puff contains E74, a complex ecdysone-inducible gene that encodes two ets-related proteins

Kenneth C. Burtis, Carl S. Thummel, C. Weldon Jones, Felix D. Karim, David S. Hogness

Research output: Contribution to journalArticle

287 Citations (Scopus)

Abstract

We have isolated an ecdysone-inducible gene, E74, from the early puff at position 74EF in the Drosophila polytene chromosomes. We show that E74 consists of three nested transcription units that derive from unique promoters but share a single polyadenylation site. The 60 kb E74A unit is directly induced by ecdysone and leads to the synthesis of a 6.0 kb mRNA that contains an unusually long 5′ leader (1891 nucleotides) with 17 short ORFs. Within the fifth of the seven E74A introns are two E74B promoters that direct the synthesis of 4.8 and 5.1 kb mRNAs. The nested arrangement of these transcription units leads to the formation of two E74 proteins, each with a unique N-terminal domain joined to a common C-terminal domain. The unique N-terminal domains contain regions rich in acidic amino acids while the C-terminal domain is rich in basic amino acids and is very similar to proteins encoded by the ets proto-oncogene superfamily.

Original languageEnglish (US)
Pages (from-to)85-99
Number of pages15
JournalCell
Volume61
Issue number1
DOIs
StatePublished - Apr 6 1990
Externally publishedYes

Fingerprint

Ecdysone
Transcription
Drosophila
Genes
Proto-Oncogene Proteins c-ets
Polytene Chromosomes
Acidic Amino Acids
Basic Amino Acids
Messenger RNA
Polyadenylation
Chromosomes
Introns
Open Reading Frames
Proteins
Nucleotides

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

The Drosophila 74EF early puff contains E74, a complex ecdysone-inducible gene that encodes two ets-related proteins. / Burtis, Kenneth C.; Thummel, Carl S.; Jones, C. Weldon; Karim, Felix D.; Hogness, David S.

In: Cell, Vol. 61, No. 1, 06.04.1990, p. 85-99.

Research output: Contribution to journalArticle

Burtis, Kenneth C. ; Thummel, Carl S. ; Jones, C. Weldon ; Karim, Felix D. ; Hogness, David S. / The Drosophila 74EF early puff contains E74, a complex ecdysone-inducible gene that encodes two ets-related proteins. In: Cell. 1990 ; Vol. 61, No. 1. pp. 85-99.
@article{080d1890033d4ea0947154a1b5305075,
title = "The Drosophila 74EF early puff contains E74, a complex ecdysone-inducible gene that encodes two ets-related proteins",
abstract = "We have isolated an ecdysone-inducible gene, E74, from the early puff at position 74EF in the Drosophila polytene chromosomes. We show that E74 consists of three nested transcription units that derive from unique promoters but share a single polyadenylation site. The 60 kb E74A unit is directly induced by ecdysone and leads to the synthesis of a 6.0 kb mRNA that contains an unusually long 5′ leader (1891 nucleotides) with 17 short ORFs. Within the fifth of the seven E74A introns are two E74B promoters that direct the synthesis of 4.8 and 5.1 kb mRNAs. The nested arrangement of these transcription units leads to the formation of two E74 proteins, each with a unique N-terminal domain joined to a common C-terminal domain. The unique N-terminal domains contain regions rich in acidic amino acids while the C-terminal domain is rich in basic amino acids and is very similar to proteins encoded by the ets proto-oncogene superfamily.",
author = "Burtis, {Kenneth C.} and Thummel, {Carl S.} and Jones, {C. Weldon} and Karim, {Felix D.} and Hogness, {David S.}",
year = "1990",
month = "4",
day = "6",
doi = "10.1016/0092-8674(90)90217-3",
language = "English (US)",
volume = "61",
pages = "85--99",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "1",

}

TY - JOUR

T1 - The Drosophila 74EF early puff contains E74, a complex ecdysone-inducible gene that encodes two ets-related proteins

AU - Burtis, Kenneth C.

AU - Thummel, Carl S.

AU - Jones, C. Weldon

AU - Karim, Felix D.

AU - Hogness, David S.

PY - 1990/4/6

Y1 - 1990/4/6

N2 - We have isolated an ecdysone-inducible gene, E74, from the early puff at position 74EF in the Drosophila polytene chromosomes. We show that E74 consists of three nested transcription units that derive from unique promoters but share a single polyadenylation site. The 60 kb E74A unit is directly induced by ecdysone and leads to the synthesis of a 6.0 kb mRNA that contains an unusually long 5′ leader (1891 nucleotides) with 17 short ORFs. Within the fifth of the seven E74A introns are two E74B promoters that direct the synthesis of 4.8 and 5.1 kb mRNAs. The nested arrangement of these transcription units leads to the formation of two E74 proteins, each with a unique N-terminal domain joined to a common C-terminal domain. The unique N-terminal domains contain regions rich in acidic amino acids while the C-terminal domain is rich in basic amino acids and is very similar to proteins encoded by the ets proto-oncogene superfamily.

AB - We have isolated an ecdysone-inducible gene, E74, from the early puff at position 74EF in the Drosophila polytene chromosomes. We show that E74 consists of three nested transcription units that derive from unique promoters but share a single polyadenylation site. The 60 kb E74A unit is directly induced by ecdysone and leads to the synthesis of a 6.0 kb mRNA that contains an unusually long 5′ leader (1891 nucleotides) with 17 short ORFs. Within the fifth of the seven E74A introns are two E74B promoters that direct the synthesis of 4.8 and 5.1 kb mRNAs. The nested arrangement of these transcription units leads to the formation of two E74 proteins, each with a unique N-terminal domain joined to a common C-terminal domain. The unique N-terminal domains contain regions rich in acidic amino acids while the C-terminal domain is rich in basic amino acids and is very similar to proteins encoded by the ets proto-oncogene superfamily.

UR - http://www.scopus.com/inward/record.url?scp=0025237056&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025237056&partnerID=8YFLogxK

U2 - 10.1016/0092-8674(90)90217-3

DO - 10.1016/0092-8674(90)90217-3

M3 - Article

VL - 61

SP - 85

EP - 99

JO - Cell

JF - Cell

SN - 0092-8674

IS - 1

ER -