The doublesex proteins of Drosophila melanogaster bind directly to a sex-specific yolk protein gene enhancer

Kenneth C. Burtis, Karen T. Coschigano, Bruce S. Baker, Pieter C. Wensink

Research output: Contribution to journalArticle

144 Citations (Scopus)

Abstract

The doublesex (dsx) gene of Drosophila melanogaster encodes both male-specific and female-specific polypeptides, whose synthesis is regulated by alternative sex-specific splicing of the primary dsx transcript. The alternative splicing of the dsx mRNA is the last known step in a cascade of regulatory gene interactions that involves both transcriptional and post-transcriptional mechanisms. Genetic studies have shown that the products of the dsx locus are required for correct somatic sexual differentiation of both sexes, and have suggested that each dsx product functions by repressing expression of terminal differentiation genes specific to the opposite sex. However, these studies have not shown whether the dsx gene products function directly to regulate the expression of target genes, or indirectly through another regulatory gene. We report here that the male- and female-specific DSX proteins, expressed in E.coli, bind directly and specifically in vitro to three DNA sequences located in an enhancer region that regulates female-specific expression of two target genes, the yolk protein genes 1 and 2. This result suggests strongly that dsx is a final regulatory gene in the hierarchy of regulatory genes controlling somatic sexual differentiation.

Original languageEnglish (US)
Pages (from-to)2577-2582
Number of pages6
JournalEMBO Journal
Volume10
Issue number9
StatePublished - 1991

Fingerprint

Drosophila Proteins
Egg Proteins
Regulator Genes
Genes
Sex Differentiation
Alternative Splicing
Drosophila melanogaster
Proteins
Escherichia coli
Gene Expression
Messenger RNA
Peptides
Drosophila DSX protein
DNA sequences

Keywords

  • Doublesex
  • Enhancer
  • Repressor
  • Sex determination
  • Yolk protein

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Burtis, K. C., Coschigano, K. T., Baker, B. S., & Wensink, P. C. (1991). The doublesex proteins of Drosophila melanogaster bind directly to a sex-specific yolk protein gene enhancer. EMBO Journal, 10(9), 2577-2582.

The doublesex proteins of Drosophila melanogaster bind directly to a sex-specific yolk protein gene enhancer. / Burtis, Kenneth C.; Coschigano, Karen T.; Baker, Bruce S.; Wensink, Pieter C.

In: EMBO Journal, Vol. 10, No. 9, 1991, p. 2577-2582.

Research output: Contribution to journalArticle

Burtis, KC, Coschigano, KT, Baker, BS & Wensink, PC 1991, 'The doublesex proteins of Drosophila melanogaster bind directly to a sex-specific yolk protein gene enhancer', EMBO Journal, vol. 10, no. 9, pp. 2577-2582.
Burtis, Kenneth C. ; Coschigano, Karen T. ; Baker, Bruce S. ; Wensink, Pieter C. / The doublesex proteins of Drosophila melanogaster bind directly to a sex-specific yolk protein gene enhancer. In: EMBO Journal. 1991 ; Vol. 10, No. 9. pp. 2577-2582.
@article{0a6c4711dd7b45d09db95f38244e1372,
title = "The doublesex proteins of Drosophila melanogaster bind directly to a sex-specific yolk protein gene enhancer",
abstract = "The doublesex (dsx) gene of Drosophila melanogaster encodes both male-specific and female-specific polypeptides, whose synthesis is regulated by alternative sex-specific splicing of the primary dsx transcript. The alternative splicing of the dsx mRNA is the last known step in a cascade of regulatory gene interactions that involves both transcriptional and post-transcriptional mechanisms. Genetic studies have shown that the products of the dsx locus are required for correct somatic sexual differentiation of both sexes, and have suggested that each dsx product functions by repressing expression of terminal differentiation genes specific to the opposite sex. However, these studies have not shown whether the dsx gene products function directly to regulate the expression of target genes, or indirectly through another regulatory gene. We report here that the male- and female-specific DSX proteins, expressed in E.coli, bind directly and specifically in vitro to three DNA sequences located in an enhancer region that regulates female-specific expression of two target genes, the yolk protein genes 1 and 2. This result suggests strongly that dsx is a final regulatory gene in the hierarchy of regulatory genes controlling somatic sexual differentiation.",
keywords = "Doublesex, Enhancer, Repressor, Sex determination, Yolk protein",
author = "Burtis, {Kenneth C.} and Coschigano, {Karen T.} and Baker, {Bruce S.} and Wensink, {Pieter C.}",
year = "1991",
language = "English (US)",
volume = "10",
pages = "2577--2582",
journal = "EMBO Journal",
issn = "0261-4189",
publisher = "Nature Publishing Group",
number = "9",

}

TY - JOUR

T1 - The doublesex proteins of Drosophila melanogaster bind directly to a sex-specific yolk protein gene enhancer

AU - Burtis, Kenneth C.

AU - Coschigano, Karen T.

AU - Baker, Bruce S.

AU - Wensink, Pieter C.

PY - 1991

Y1 - 1991

N2 - The doublesex (dsx) gene of Drosophila melanogaster encodes both male-specific and female-specific polypeptides, whose synthesis is regulated by alternative sex-specific splicing of the primary dsx transcript. The alternative splicing of the dsx mRNA is the last known step in a cascade of regulatory gene interactions that involves both transcriptional and post-transcriptional mechanisms. Genetic studies have shown that the products of the dsx locus are required for correct somatic sexual differentiation of both sexes, and have suggested that each dsx product functions by repressing expression of terminal differentiation genes specific to the opposite sex. However, these studies have not shown whether the dsx gene products function directly to regulate the expression of target genes, or indirectly through another regulatory gene. We report here that the male- and female-specific DSX proteins, expressed in E.coli, bind directly and specifically in vitro to three DNA sequences located in an enhancer region that regulates female-specific expression of two target genes, the yolk protein genes 1 and 2. This result suggests strongly that dsx is a final regulatory gene in the hierarchy of regulatory genes controlling somatic sexual differentiation.

AB - The doublesex (dsx) gene of Drosophila melanogaster encodes both male-specific and female-specific polypeptides, whose synthesis is regulated by alternative sex-specific splicing of the primary dsx transcript. The alternative splicing of the dsx mRNA is the last known step in a cascade of regulatory gene interactions that involves both transcriptional and post-transcriptional mechanisms. Genetic studies have shown that the products of the dsx locus are required for correct somatic sexual differentiation of both sexes, and have suggested that each dsx product functions by repressing expression of terminal differentiation genes specific to the opposite sex. However, these studies have not shown whether the dsx gene products function directly to regulate the expression of target genes, or indirectly through another regulatory gene. We report here that the male- and female-specific DSX proteins, expressed in E.coli, bind directly and specifically in vitro to three DNA sequences located in an enhancer region that regulates female-specific expression of two target genes, the yolk protein genes 1 and 2. This result suggests strongly that dsx is a final regulatory gene in the hierarchy of regulatory genes controlling somatic sexual differentiation.

KW - Doublesex

KW - Enhancer

KW - Repressor

KW - Sex determination

KW - Yolk protein

UR - http://www.scopus.com/inward/record.url?scp=0025734112&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025734112&partnerID=8YFLogxK

M3 - Article

VL - 10

SP - 2577

EP - 2582

JO - EMBO Journal

JF - EMBO Journal

SN - 0261-4189

IS - 9

ER -