Abstract
The environmental contaminant 2,3,7,8-tetra-chlorodibenzo-p-dioxin produces its biological effects by binding to an intracellular protein (the Ah receptor). The dioxin-Ah receptor complex activates cytochrome P1-450 gene transcription by interacting with dioxin-responsive enhancers. Here, we report that the dioxin-Ah receptor complex binds to DNA containing the 'core' sequence 5'-TA/TGCGTG-3', which is present in each of three receptor-dependent enhancers. Functional analyses indicate that binding of the liganded Ah receptor to the core sequence fails to generate an active enhancer and that nucleotides flanking the core sequence must contribute to enhancer function and dioxin action.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 17221-17224 |
| Number of pages | 4 |
| Journal | Journal of Biological Chemistry |
| Volume | 263 |
| Issue number | 33 |
| State | Published - 1988 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
Cite this
The DNA recognition site for the dioxin-Ah receptor complex. Nucleotide sequence and functional analysis. / Denison, M. S.; Fisher, J. M.; Whitlock, J. P.
In: Journal of Biological Chemistry, Vol. 263, No. 33, 1988, p. 17221-17224.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The DNA recognition site for the dioxin-Ah receptor complex. Nucleotide sequence and functional analysis
AU - Denison, M. S.
AU - Fisher, J. M.
AU - Whitlock, J. P.
PY - 1988
Y1 - 1988
N2 - The environmental contaminant 2,3,7,8-tetra-chlorodibenzo-p-dioxin produces its biological effects by binding to an intracellular protein (the Ah receptor). The dioxin-Ah receptor complex activates cytochrome P1-450 gene transcription by interacting with dioxin-responsive enhancers. Here, we report that the dioxin-Ah receptor complex binds to DNA containing the 'core' sequence 5'-TA/TGCGTG-3', which is present in each of three receptor-dependent enhancers. Functional analyses indicate that binding of the liganded Ah receptor to the core sequence fails to generate an active enhancer and that nucleotides flanking the core sequence must contribute to enhancer function and dioxin action.
AB - The environmental contaminant 2,3,7,8-tetra-chlorodibenzo-p-dioxin produces its biological effects by binding to an intracellular protein (the Ah receptor). The dioxin-Ah receptor complex activates cytochrome P1-450 gene transcription by interacting with dioxin-responsive enhancers. Here, we report that the dioxin-Ah receptor complex binds to DNA containing the 'core' sequence 5'-TA/TGCGTG-3', which is present in each of three receptor-dependent enhancers. Functional analyses indicate that binding of the liganded Ah receptor to the core sequence fails to generate an active enhancer and that nucleotides flanking the core sequence must contribute to enhancer function and dioxin action.
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UR - http://www.scopus.com/inward/citedby.url?scp=0024210678&partnerID=8YFLogxK
M3 - Article
C2 - 2846558
AN - SCOPUS:0024210678
VL - 263
SP - 17221
EP - 17224
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 33
ER -