The DNA recognition site for the dioxin-Ah receptor complex. Nucleotide sequence and functional analysis

M. S. Denison, J. M. Fisher, J. P. Whitlock

Research output: Contribution to journalArticle

385 Scopus citations

Abstract

The environmental contaminant 2,3,7,8-tetra-chlorodibenzo-p-dioxin produces its biological effects by binding to an intracellular protein (the Ah receptor). The dioxin-Ah receptor complex activates cytochrome P1-450 gene transcription by interacting with dioxin-responsive enhancers. Here, we report that the dioxin-Ah receptor complex binds to DNA containing the 'core' sequence 5'-TA/TGCGTG-3', which is present in each of three receptor-dependent enhancers. Functional analyses indicate that binding of the liganded Ah receptor to the core sequence fails to generate an active enhancer and that nucleotides flanking the core sequence must contribute to enhancer function and dioxin action.

Original languageEnglish (US)
Pages (from-to)17221-17224
Number of pages4
JournalJournal of Biological Chemistry
Volume263
Issue number33
StatePublished - 1988
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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