The distribution of the main intrinsic membrane polypeptide in ocular Lens

Paul G FitzGerald, Dean Bok, Joseph Horwitz

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The Main Intrinsic Polypeptide (MIP) of the ocular lens fiber cell plasma membrane was immunocytochemically localized at the ultrastructural level on ultrathin frozen sections of rat lens, and on extracted, gradient-purified bovine lens membranes. The results indicate that both the junctional and non-junctional membrane domains of the cortical lens fiber cell are MIP immunoreactive. Frozen thin section immunocytochemistry of the lens epithelium and hepatocytes, also using anti-MIP antibodies, revealed that these cells, and their intercellular junctions, are not MIP-immunoreactive. From these findings we conclude that 1) MIP, a putative fiber cell junctional protein, is present throughout the plasma membrane of the lens fiber cell, and is not confined to the fiber cell junctional domain, 2) MIP is not a detectable component of the lens epithelial cell membrane, or its intercellular junctions, 3) MIP is not detectable in gap junctions of hepatocytes.

Original languageEnglish (US)
Pages (from-to)1203-1218
Number of pages16
JournalCurrent Eye Research
Volume4
Issue number11
DOIs
StatePublished - 1985

Fingerprint

Crystalline Lens
Lenses
Peptides
Membranes
Cell Membrane
Intercellular Junctions
Frozen Sections
Hepatocytes
Gap Junctions
Epithelium
Epithelial Cells
Immunohistochemistry
Antibodies

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

The distribution of the main intrinsic membrane polypeptide in ocular Lens. / FitzGerald, Paul G; Bok, Dean; Horwitz, Joseph.

In: Current Eye Research, Vol. 4, No. 11, 1985, p. 1203-1218.

Research output: Contribution to journalArticle

FitzGerald, Paul G ; Bok, Dean ; Horwitz, Joseph. / The distribution of the main intrinsic membrane polypeptide in ocular Lens. In: Current Eye Research. 1985 ; Vol. 4, No. 11. pp. 1203-1218.
@article{5e152be2458a41b19b015256dba67c60,
title = "The distribution of the main intrinsic membrane polypeptide in ocular Lens",
abstract = "The Main Intrinsic Polypeptide (MIP) of the ocular lens fiber cell plasma membrane was immunocytochemically localized at the ultrastructural level on ultrathin frozen sections of rat lens, and on extracted, gradient-purified bovine lens membranes. The results indicate that both the junctional and non-junctional membrane domains of the cortical lens fiber cell are MIP immunoreactive. Frozen thin section immunocytochemistry of the lens epithelium and hepatocytes, also using anti-MIP antibodies, revealed that these cells, and their intercellular junctions, are not MIP-immunoreactive. From these findings we conclude that 1) MIP, a putative fiber cell junctional protein, is present throughout the plasma membrane of the lens fiber cell, and is not confined to the fiber cell junctional domain, 2) MIP is not a detectable component of the lens epithelial cell membrane, or its intercellular junctions, 3) MIP is not detectable in gap junctions of hepatocytes.",
author = "FitzGerald, {Paul G} and Dean Bok and Joseph Horwitz",
year = "1985",
doi = "10.3109/02713688509003365",
language = "English (US)",
volume = "4",
pages = "1203--1218",
journal = "Current Eye Research",
issn = "0271-3683",
publisher = "Informa Healthcare",
number = "11",

}

TY - JOUR

T1 - The distribution of the main intrinsic membrane polypeptide in ocular Lens

AU - FitzGerald, Paul G

AU - Bok, Dean

AU - Horwitz, Joseph

PY - 1985

Y1 - 1985

N2 - The Main Intrinsic Polypeptide (MIP) of the ocular lens fiber cell plasma membrane was immunocytochemically localized at the ultrastructural level on ultrathin frozen sections of rat lens, and on extracted, gradient-purified bovine lens membranes. The results indicate that both the junctional and non-junctional membrane domains of the cortical lens fiber cell are MIP immunoreactive. Frozen thin section immunocytochemistry of the lens epithelium and hepatocytes, also using anti-MIP antibodies, revealed that these cells, and their intercellular junctions, are not MIP-immunoreactive. From these findings we conclude that 1) MIP, a putative fiber cell junctional protein, is present throughout the plasma membrane of the lens fiber cell, and is not confined to the fiber cell junctional domain, 2) MIP is not a detectable component of the lens epithelial cell membrane, or its intercellular junctions, 3) MIP is not detectable in gap junctions of hepatocytes.

AB - The Main Intrinsic Polypeptide (MIP) of the ocular lens fiber cell plasma membrane was immunocytochemically localized at the ultrastructural level on ultrathin frozen sections of rat lens, and on extracted, gradient-purified bovine lens membranes. The results indicate that both the junctional and non-junctional membrane domains of the cortical lens fiber cell are MIP immunoreactive. Frozen thin section immunocytochemistry of the lens epithelium and hepatocytes, also using anti-MIP antibodies, revealed that these cells, and their intercellular junctions, are not MIP-immunoreactive. From these findings we conclude that 1) MIP, a putative fiber cell junctional protein, is present throughout the plasma membrane of the lens fiber cell, and is not confined to the fiber cell junctional domain, 2) MIP is not a detectable component of the lens epithelial cell membrane, or its intercellular junctions, 3) MIP is not detectable in gap junctions of hepatocytes.

UR - http://www.scopus.com/inward/record.url?scp=0022218284&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022218284&partnerID=8YFLogxK

U2 - 10.3109/02713688509003365

DO - 10.3109/02713688509003365

M3 - Article

C2 - 3907985

AN - SCOPUS:0022218284

VL - 4

SP - 1203

EP - 1218

JO - Current Eye Research

JF - Current Eye Research

SN - 0271-3683

IS - 11

ER -