The distribution of tenascin coincides with pathways of neural crest cell migration

E. J. Mackie, Richard P Tucker, W. Halfter, R. Chiquet-Ehrismann, H. H. Epperlein

Research output: Contribution to journalArticle

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Abstract

The distribution of the extracellular matrix (ECM) glycoprotein, tenascin, has been compared with that of fibronectin in neural crest migration pathways of Xenopus laevis, quail and rat embryos. In all species studied, the distribution of tenascin, examined by immunohistochemistry, was more closely correlated with pathways of migration than that of fibronectin, which is known to be important for neural crest migration. In Xenopus laevis embryos, anti-tenascin stained the dorsal fin matrix and ECM along the ventral route of migration, but not the ECM found laterally between the ectoderm and somites where neural crest cells do not migrate. In quail embryos, the appearance of tenascin in neural crest pathways was well correlated with the anterior-to-posterior wave of migration. The distribution of tenascin within somites was compared with that of the neural crest marker, HNK-1, in quail embryos. In the dorsal halves of quail somites which contained migrating neural crest cells, the predominant tenascin staining was in the anterior halves of the somites, codistributed with the migrating cells. In rat embryos, tenascin was detectable in the somites only in the anterior halves. Tenascin was not detectable in the matrix of cultured quail neural crest cells, but was in the matrix surrounding somite and notochord cells in vitro. Neural crest cells cultured on a substratum of tenascin did not spread and were rounded. We propose that tenascin is an important factor controlling neural crest morphogenesis, perhaps by modifying the interaction of neural crest cells with fibronectin.

Original languageEnglish (US)
Pages (from-to)237-250
Number of pages14
JournalDevelopment
Volume102
Issue number1
StatePublished - 1988
Externally publishedYes

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Tenascin
Neural Crest
Cell Movement
Somites
Quail
Embryonic Structures
Fibronectins
Extracellular Matrix
Xenopus laevis
Notochord
Neural Pathways
Ectoderm
Morphogenesis
Glycoproteins
Immunohistochemistry
Staining and Labeling

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

Mackie, E. J., Tucker, R. P., Halfter, W., Chiquet-Ehrismann, R., & Epperlein, H. H. (1988). The distribution of tenascin coincides with pathways of neural crest cell migration. Development, 102(1), 237-250.

The distribution of tenascin coincides with pathways of neural crest cell migration. / Mackie, E. J.; Tucker, Richard P; Halfter, W.; Chiquet-Ehrismann, R.; Epperlein, H. H.

In: Development, Vol. 102, No. 1, 1988, p. 237-250.

Research output: Contribution to journalArticle

Mackie, EJ, Tucker, RP, Halfter, W, Chiquet-Ehrismann, R & Epperlein, HH 1988, 'The distribution of tenascin coincides with pathways of neural crest cell migration', Development, vol. 102, no. 1, pp. 237-250.
Mackie EJ, Tucker RP, Halfter W, Chiquet-Ehrismann R, Epperlein HH. The distribution of tenascin coincides with pathways of neural crest cell migration. Development. 1988;102(1):237-250.
Mackie, E. J. ; Tucker, Richard P ; Halfter, W. ; Chiquet-Ehrismann, R. ; Epperlein, H. H. / The distribution of tenascin coincides with pathways of neural crest cell migration. In: Development. 1988 ; Vol. 102, No. 1. pp. 237-250.
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