Abstract
I have studied the temporal and spatial regulation of the expression of the extracellular matrix protein tenascin in the developing chick brain using immunological and in situ hybridization methods. Tenascin is barely detectable by immuno‐slot blot analysis in the E4 telencephalon, but increases rapidly in abundance until E14, when the level of expression tapers off. The late appearance of tenascin was confirmed by immunohistochemistry. Tenascin can not be detected at E4, but at E7 is distributed homogeneously in most parts of the central nervous system. Tenascin is much less abundant in the retina than in other brain areas. As the avian retina is devoid of both vasculature and fibronectin, this supports the notion that tenascin may mask fibronectin from migrating growth cones and cell bodies. I have also used in situ hybridization with a cDNA probe to determine the origins of tenascin in the developing nervous system. Tenascin transcript is found in regions of glial proliferation and the cell bodies of radial glia. In contrast, tenascin appears to be made by neurons in the retina. As a low‐molecular weight splice variant of tenascin is detected on western blots of retina homogenates, different cell types may be responsible for generating the multiple forms of tenascin found in whole brain homogenates.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 78-91 |
| Number of pages | 14 |
| Journal | Journal of Experimental Zoology |
| Volume | 259 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1991 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Animal Science and Zoology
Cite this
The distribution of tenascin and its transcript in the developing avian central nervous system. / Tucker, Richard P.
In: Journal of Experimental Zoology, Vol. 259, No. 1, 1991, p. 78-91.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The distribution of tenascin and its transcript in the developing avian central nervous system
AU - Tucker, Richard P
PY - 1991
Y1 - 1991
N2 - I have studied the temporal and spatial regulation of the expression of the extracellular matrix protein tenascin in the developing chick brain using immunological and in situ hybridization methods. Tenascin is barely detectable by immuno‐slot blot analysis in the E4 telencephalon, but increases rapidly in abundance until E14, when the level of expression tapers off. The late appearance of tenascin was confirmed by immunohistochemistry. Tenascin can not be detected at E4, but at E7 is distributed homogeneously in most parts of the central nervous system. Tenascin is much less abundant in the retina than in other brain areas. As the avian retina is devoid of both vasculature and fibronectin, this supports the notion that tenascin may mask fibronectin from migrating growth cones and cell bodies. I have also used in situ hybridization with a cDNA probe to determine the origins of tenascin in the developing nervous system. Tenascin transcript is found in regions of glial proliferation and the cell bodies of radial glia. In contrast, tenascin appears to be made by neurons in the retina. As a low‐molecular weight splice variant of tenascin is detected on western blots of retina homogenates, different cell types may be responsible for generating the multiple forms of tenascin found in whole brain homogenates.
AB - I have studied the temporal and spatial regulation of the expression of the extracellular matrix protein tenascin in the developing chick brain using immunological and in situ hybridization methods. Tenascin is barely detectable by immuno‐slot blot analysis in the E4 telencephalon, but increases rapidly in abundance until E14, when the level of expression tapers off. The late appearance of tenascin was confirmed by immunohistochemistry. Tenascin can not be detected at E4, but at E7 is distributed homogeneously in most parts of the central nervous system. Tenascin is much less abundant in the retina than in other brain areas. As the avian retina is devoid of both vasculature and fibronectin, this supports the notion that tenascin may mask fibronectin from migrating growth cones and cell bodies. I have also used in situ hybridization with a cDNA probe to determine the origins of tenascin in the developing nervous system. Tenascin transcript is found in regions of glial proliferation and the cell bodies of radial glia. In contrast, tenascin appears to be made by neurons in the retina. As a low‐molecular weight splice variant of tenascin is detected on western blots of retina homogenates, different cell types may be responsible for generating the multiple forms of tenascin found in whole brain homogenates.
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UR - http://www.scopus.com/inward/citedby.url?scp=84989640550&partnerID=8YFLogxK
U2 - 10.1002/jez.1402590111
DO - 10.1002/jez.1402590111
M3 - Article
AN - SCOPUS:84989640550
VL - 259
SP - 78
EP - 91
JO - Journal of Experimental Zoology
JF - Journal of Experimental Zoology
SN - 1552-5007
IS - 1
ER -