The distribution of acetylcholinesterase in the hippocampal formation of the monkey

I. Bakst, David G Amaral

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In order to study the distribution of acetylcholinesterase (AChE) in the primate hippocampal formation, we have stained serial sections through the brains of nine macaque monkeys for AChE by two variants of the Koelle acetylthiocholine method. We have found a distinctive pattern of staining in the hippocampal formation which varies in intensity both from region to region, and along rostrocaudal and radial gradients within each region. In the dentate gyrus, there is intense staining of the inner one-third of the molecular layer with much lighter staining in the rest of the molecular layer except for a moderately stained band at its outer edge. In the caudal half of the dentate gyrus, the inner portion of the molecular layer is less intensely stained though there is a distinctly denser band of staining just above, and partly within, the superficial margin of the granule cell layer. The granule cells are unstained but there are AChE-positive fibers which run through the granule cell layer to the molecular layer. The hilar region of the dentate gyrus has a narrow band of heavy staining (which corresponds to an acellular layer in Nissl-stained sections) just subjacent to the granule cell layer; the remainder of the hilus, where most of the hilar cells reside, is less intensely stained and at caudal levels is almost entirely unstained. The regio inferior, regio superior, subiculum, presubiculum, parasubiculum, lateral entorhinal cortex and medial entorhinal cortex were also studied.

Original languageEnglish (US)
Pages (from-to)344-371
Number of pages28
JournalJournal of Comparative Neurology
Issue number3
StatePublished - 1984
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience(all)


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