The different interactions of lysine and arginine side chains with lipid membranes

Libo Li, Igor Vorobyov, Toby W. Allen

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

The basic amino acids lysine (Lys) and arginine (Arg) play important roles in membrane protein activity, the sensing of membrane voltages, and the actions of antimicrobial, toxin, and cell-penetrating peptides. These roles are thought to stem from the strong interactions and disruptive influences of these amino acids on lipid membranes. In this study, we employ fully atomistic molecular dynamics simulations to observe, quantify, and compare the interactions of Lys and Arg with saturated phosphatidylcholine membranes of different thickness. We make use of both charged (methylammonium and methylguanidinium) and neutral (methylamine and methylguanidine) analogue molecules, as well as Lys and Arg side chains on transmembrane helix models. We find that the free energy barrier experienced by a charged Lys crossing the membrane is strikingly similar to that of a charged Arg (to within 2 kcal/mol), despite the two having different chemistries, H-bonding capability, and hydration free energies that differ by ∼10 kcal/mol. In comparison, the barrier for neutral Arg is higher than that for neutral Lys by around 5 kcal/mol, being more selective than that for the charged species. This can be explained by the different transport mechanisms for charged or neutral amino acid side chains in the membrane, involving membrane deformations or simple dehydration, respectively. As a consequence, we demonstrate that Lys would be deprotonated in the membrane, whereas Arg would maintain its charge. Our simulations also reveal that Arg attracts more phosphate and water in the membrane, and can form extensive H-bonding with its five H-bond donors to stabilize Arg-phosphate clusters. This leads to enhanced interfacial binding and membrane perturbations, including the appearance of a trans-membrane pore in a thinner membrane. These results highlight the special role played by Arg as an amino acid to bind to, disrupt, and permeabilize lipid membranes, as well as to sense voltages for a range of peptide and protein activities in nature and in engineered bionanodevices.

Original languageEnglish (US)
Pages (from-to)11906-11920
Number of pages15
JournalJournal of Physical Chemistry B
Volume117
Issue number40
DOIs
StatePublished - Oct 10 2013

Fingerprint

Arginine
lysine
Membrane Lipids
Lysine
lipids
membranes
Membranes
interactions
Amino acids
amino acids
Free energy
Peptides
Methylguanidine
Phosphates
Cell-Penetrating Peptides
peptides
Neutral Amino Acids
Amino Acids
phosphates
Basic Amino Acids

ASJC Scopus subject areas

  • Surfaces, Coatings and Films
  • Physical and Theoretical Chemistry
  • Materials Chemistry

Cite this

The different interactions of lysine and arginine side chains with lipid membranes. / Li, Libo; Vorobyov, Igor; Allen, Toby W.

In: Journal of Physical Chemistry B, Vol. 117, No. 40, 10.10.2013, p. 11906-11920.

Research output: Contribution to journalArticle

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