The diagnostic and prognostic utility of claudin expression in renal cell neoplasms

Mirna Lechpammer, Murray B. Resnick, Edmond Sabo, Evgeny Yakirevich, Wesley O. Greaves, Katherine T. Sciandra, Rosemarie Tavares, Lelia C. Noble, Ronald A. DeLellis, Li J. Wang

Research output: Contribution to journalArticle

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Abstract

This study evaluated the expression patterns of claudins 1, 3, 4, 7, and 8 in human renal cell carcinomas and oncocytomas and correlated expression with patient prognosis. Tissue microarrays were created from paraffin-embedded tissue samples from 141 patients with renal cell carcinomas or oncocytoma (90 clear cell, 22 papillary, 17 chromophobe renal cell carcinomas, and 12 oncocytomas). The staining pattern for claudins 3, 4, 7, and 8 was membranous and/or cytoplasmic, whereas claudin 1 was predominantly membranous in both nonneoplastic renal tissue and tumors. Negative to weak claudin 3 staining was predominantly detected in Fuhrman's grade 1 and 2 clear cell renal cell carcinomas (78%; P=0.016), suggesting that upregulation of claudin 3 potentially occurs concomitantly with increasing grade of clear cell renal cell carcinomas. In addition, Kaplan-Meier univariate analysis showed a significant inverse correlation between moderate to strong claudin 3 and 4 expression with overall survival in clear cell renal cell carcinomas (P=0.038 and P=0.031). Moderate to strong claudin 7 expression was significantly more common in chromophobe renal cell carcinomas (94%) than in oncocytomas (55%; P=0.041). Claudin 8 staining was moderate to strong in 92% of oncocytomas, which differentiated them from papillary and clear cell renal cell carcinomas (14 and 12%; both P=0.0001). Only negative to weak claudin 8 staining was detected in all chromophobe renal cell carcinomas, whereas there were no claudin 8 negative oncocytomas and 8% exhibited a weak staining pattern (P=0.0001). Due to their distinctive expression patterns, claudins 7 and 8 can be used as useful immunohistochemical markers for the separation of chromophobe renal cell carcinomas from oncocytomas, whereas claudins 3 and 4 may serve as indicators of prognosis in clear cell renal cell carcinomas.

Original languageEnglish (US)
Pages (from-to)1320-1329
Number of pages10
JournalModern Pathology
Volume21
Issue number11
DOIs
StatePublished - Nov 2008
Externally publishedYes

Fingerprint

Kidney Neoplasms
Renal Cell Carcinoma
Oxyphilic Adenoma
Claudins
Claudin-3
Staining and Labeling
Claudin-4
Claudin-1
Kaplan-Meier Estimate
Paraffin
Up-Regulation
Kidney

Keywords

  • Carcinoma
  • Chromophobe renal cell carcinoma
  • Claudins
  • Kidney
  • Oncocytoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Lechpammer, M., Resnick, M. B., Sabo, E., Yakirevich, E., Greaves, W. O., Sciandra, K. T., ... Wang, L. J. (2008). The diagnostic and prognostic utility of claudin expression in renal cell neoplasms. Modern Pathology, 21(11), 1320-1329. https://doi.org/10.1038/modpathol.2008.116

The diagnostic and prognostic utility of claudin expression in renal cell neoplasms. / Lechpammer, Mirna; Resnick, Murray B.; Sabo, Edmond; Yakirevich, Evgeny; Greaves, Wesley O.; Sciandra, Katherine T.; Tavares, Rosemarie; Noble, Lelia C.; DeLellis, Ronald A.; Wang, Li J.

In: Modern Pathology, Vol. 21, No. 11, 11.2008, p. 1320-1329.

Research output: Contribution to journalArticle

Lechpammer, M, Resnick, MB, Sabo, E, Yakirevich, E, Greaves, WO, Sciandra, KT, Tavares, R, Noble, LC, DeLellis, RA & Wang, LJ 2008, 'The diagnostic and prognostic utility of claudin expression in renal cell neoplasms', Modern Pathology, vol. 21, no. 11, pp. 1320-1329. https://doi.org/10.1038/modpathol.2008.116
Lechpammer, Mirna ; Resnick, Murray B. ; Sabo, Edmond ; Yakirevich, Evgeny ; Greaves, Wesley O. ; Sciandra, Katherine T. ; Tavares, Rosemarie ; Noble, Lelia C. ; DeLellis, Ronald A. ; Wang, Li J. / The diagnostic and prognostic utility of claudin expression in renal cell neoplasms. In: Modern Pathology. 2008 ; Vol. 21, No. 11. pp. 1320-1329.
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abstract = "This study evaluated the expression patterns of claudins 1, 3, 4, 7, and 8 in human renal cell carcinomas and oncocytomas and correlated expression with patient prognosis. Tissue microarrays were created from paraffin-embedded tissue samples from 141 patients with renal cell carcinomas or oncocytoma (90 clear cell, 22 papillary, 17 chromophobe renal cell carcinomas, and 12 oncocytomas). The staining pattern for claudins 3, 4, 7, and 8 was membranous and/or cytoplasmic, whereas claudin 1 was predominantly membranous in both nonneoplastic renal tissue and tumors. Negative to weak claudin 3 staining was predominantly detected in Fuhrman's grade 1 and 2 clear cell renal cell carcinomas (78{\%}; P=0.016), suggesting that upregulation of claudin 3 potentially occurs concomitantly with increasing grade of clear cell renal cell carcinomas. In addition, Kaplan-Meier univariate analysis showed a significant inverse correlation between moderate to strong claudin 3 and 4 expression with overall survival in clear cell renal cell carcinomas (P=0.038 and P=0.031). Moderate to strong claudin 7 expression was significantly more common in chromophobe renal cell carcinomas (94{\%}) than in oncocytomas (55{\%}; P=0.041). Claudin 8 staining was moderate to strong in 92{\%} of oncocytomas, which differentiated them from papillary and clear cell renal cell carcinomas (14 and 12{\%}; both P=0.0001). Only negative to weak claudin 8 staining was detected in all chromophobe renal cell carcinomas, whereas there were no claudin 8 negative oncocytomas and 8{\%} exhibited a weak staining pattern (P=0.0001). Due to their distinctive expression patterns, claudins 7 and 8 can be used as useful immunohistochemical markers for the separation of chromophobe renal cell carcinomas from oncocytomas, whereas claudins 3 and 4 may serve as indicators of prognosis in clear cell renal cell carcinomas.",
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