Diagnóstico de la forma muy virulenta de la enfermedad infecciosa de la bolsa en pollas de reemplazo en California

Translated title of the contribution: The diagnosis of very virulent infectious bursal disease in California pullets

Simone Stoute, Daral J. Jackwood, Susan E. Sommer-Wagner, George L. Cooper, Mark L Anderson, Peter R. Woolcock, Arthur A. Bickford, Carlos G Senties-cue, Bruce R. Charlton

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

This report documents the occurrence of a very virulent infectious bursal disease virus (vvIBDV) in Northern California commercial brown pullets. Diagnosis was made from multiple accessions from two neighboring and epidemiologically related ranches submitted to the California Animal Health and Food Safety (CAHFS) laboratory. Pullets, 11 and 14 wk of age from ranch A (rA) and ranch B (rB) respectively, were submitted from infectious bursal disease virus vaccinated flocks experiencing a drastic increase in mortality. The December 2008 outbreak resulted in 26% and 34% mortality on rA and rB respectively. Gross and histologic lesions characteristic of acute vvIBDV were observed. Gross lesions included edematous bursas, hemorrhages at the junction of the proventriculus and gizzard as well as hemorrhages on skeletal muscles. Microscopic lesions included severe lymphoid necrosis and inflammation in edematous bursas, lymphoid necrosis in thymus, spleen, Peyer's patches and cecal tonsils. Diagnosis of vvIBDV was confirmed by molecular characterization of the IBDV from bursas as well as viral pathogenicity in specific-pathogen-free birds. RT-PCR and nucleotide sequencing of the hypervariable region of the VP2 (vVP2) gene segment of the IBDV genome was performed on rA, rB and embryo passaged rA virions. The amino acids compatible with vvIBDV isolates: 222(Ala), 242(Ile), 256(Ile), 294(Ile) and 299(Ser) were reported from both ranches. In addition, nucleotide sequencing of a fragment of the VP1 gene demonstrated the viruses have the segment B genotype associated with highly pathogenic vvIBDV. Inocula of 105.5 50% egg infective dose of vvIBDV virus from rA and rB were introduced orally into two groups (g1 and g2 respectively) of 4 wk 2-day-old SPF leghorns. At 4 days postinoculation, there was 100% (22/22) morbidity in g1 and g2; 91% (20/22) mortality in g1; 100% (22/22) mortality for g2; 0% (0/20) morbidity and 0% (0/ 20) mortality was reported in the control group. This is the first occurrence of vvIBDV reported from birds in the United States.

Original languageSpanish
Pages (from-to)321-326
Number of pages6
JournalAvian Diseases
Volume53
Issue number2
DOIs
StatePublished - Jun 2009

Fingerprint

Infectious bursal disease virus
infectious bursal disease
pullets
ranching
Communicable Diseases
Mortality
lesions (animal)
Birds
hemorrhage
morbidity
Necrosis
Nucleotides
Proventriculus
necrosis
Hemorrhage
nucleotides
Viruses
Morbidity
Specific Pathogen-Free Organisms
Peyer's Patches

Keywords

  • Bursa of Fabricius
  • Lymphoid necrosis
  • Pullets
  • RT-PCR
  • Very virulent infectious bursal disease

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Food Animals
  • Immunology and Microbiology(all)

Cite this

Diagnóstico de la forma muy virulenta de la enfermedad infecciosa de la bolsa en pollas de reemplazo en California. / Stoute, Simone; Jackwood, Daral J.; Sommer-Wagner, Susan E.; Cooper, George L.; Anderson, Mark L; Woolcock, Peter R.; Bickford, Arthur A.; Senties-cue, Carlos G; Charlton, Bruce R.

In: Avian Diseases, Vol. 53, No. 2, 06.2009, p. 321-326.

Research output: Contribution to journalArticle

Stoute, Simone ; Jackwood, Daral J. ; Sommer-Wagner, Susan E. ; Cooper, George L. ; Anderson, Mark L ; Woolcock, Peter R. ; Bickford, Arthur A. ; Senties-cue, Carlos G ; Charlton, Bruce R. / Diagnóstico de la forma muy virulenta de la enfermedad infecciosa de la bolsa en pollas de reemplazo en California. In: Avian Diseases. 2009 ; Vol. 53, No. 2. pp. 321-326.
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abstract = "This report documents the occurrence of a very virulent infectious bursal disease virus (vvIBDV) in Northern California commercial brown pullets. Diagnosis was made from multiple accessions from two neighboring and epidemiologically related ranches submitted to the California Animal Health and Food Safety (CAHFS) laboratory. Pullets, 11 and 14 wk of age from ranch A (rA) and ranch B (rB) respectively, were submitted from infectious bursal disease virus vaccinated flocks experiencing a drastic increase in mortality. The December 2008 outbreak resulted in 26{\%} and 34{\%} mortality on rA and rB respectively. Gross and histologic lesions characteristic of acute vvIBDV were observed. Gross lesions included edematous bursas, hemorrhages at the junction of the proventriculus and gizzard as well as hemorrhages on skeletal muscles. Microscopic lesions included severe lymphoid necrosis and inflammation in edematous bursas, lymphoid necrosis in thymus, spleen, Peyer's patches and cecal tonsils. Diagnosis of vvIBDV was confirmed by molecular characterization of the IBDV from bursas as well as viral pathogenicity in specific-pathogen-free birds. RT-PCR and nucleotide sequencing of the hypervariable region of the VP2 (vVP2) gene segment of the IBDV genome was performed on rA, rB and embryo passaged rA virions. The amino acids compatible with vvIBDV isolates: 222(Ala), 242(Ile), 256(Ile), 294(Ile) and 299(Ser) were reported from both ranches. In addition, nucleotide sequencing of a fragment of the VP1 gene demonstrated the viruses have the segment B genotype associated with highly pathogenic vvIBDV. Inocula of 105.5 50{\%} egg infective dose of vvIBDV virus from rA and rB were introduced orally into two groups (g1 and g2 respectively) of 4 wk 2-day-old SPF leghorns. At 4 days postinoculation, there was 100{\%} (22/22) morbidity in g1 and g2; 91{\%} (20/22) mortality in g1; 100{\%} (22/22) mortality for g2; 0{\%} (0/20) morbidity and 0{\%} (0/ 20) mortality was reported in the control group. This is the first occurrence of vvIBDV reported from birds in the United States.",
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AU - Cooper, George L.

AU - Anderson, Mark L

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