The detection of conventional class I and class II I-E homologue major histocompatibility complex molecules on feline cells

Marilyn S. Pollack, Audrey Hayes, Samantha Mooney, Niels C Pedersen, Richard G. Cook

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The presence on feline cells of class I and class II I-E type major histocompatibility complex (MHC) homologues was demonstrated using cross-reacting monoclonal antibodies (mAb). The feline class I antigen homologues were detected with both immunofluorescent and biochemical techniques, using the anti-human class I mAb W6/32. The class I antigens were detected on in vitro cultured feline fibroblasts and lymphoid cells, but not on fresh lymphoid cells, apparently as a result of the association of bovine beta-2 microglobulin with feline class I heavy chains which generated the determinant(s) recognized by mAb W6/32. Class II I-E-like molecules could be detected with immunofluorescent techniques using the species cross-reactive anti-mouse I-E antibody 40D only when peripheral blood mononuclear cells were activated, for example, with the mitogens staphylococcus enterotoxin A or lipopolysaccharide. The predominant expression of I-A-like molecules by resting class II-positive feline cells could explain some of the functional difference we have seen in comparison with those of most other mammalian species.

Original languageEnglish (US)
Pages (from-to)79-91
Number of pages13
JournalVeterinary Immunology and Immunopathology
Volume19
Issue number1
DOIs
StatePublished - 1988

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology
  • veterinary(all)

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