The counterion influence on cationic lipid-mediated transfection of plasmid DNA

Alfred M. Aberle, Michael J. Bennett, Robert W. Malone, Michael H. Nantz

Research output: Contribution to journalArticle

42 Scopus citations

Abstract

A panel of DOTAP analogs was prepared by altering the anionic counterion that accompanies the trimethylammonium polar domain. The transfection of plasmid DNA into NIH3T3 cells and mouse lung was examined using the counterion analogs. The in vitro transfection activity decreased as follows: DOTAP bisulfate > trifluoromethanesulfonate ∼ iodide ∼ bromide > dihydrogenphosphate ∼ chloride ∼ acetate > sulfate. A similar activity trend was observed in vivo.

Original languageEnglish (US)
Pages (from-to)281-283
Number of pages3
JournalBiochimica et Biophysica Acta - Lipids and Lipid Metabolism
Volume1299
Issue number3
StatePublished - 1996

Keywords

  • Cationic lipid
  • Counterion effect
  • DNA
  • Gene transfer
  • Liposome
  • Transfection

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Biophysics

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