The constitutive expression of galectin-3 is downregulated in the intestinal epithelia of Crohn's disease patients, and tumour necrosis factor alpha decreases the level of galectin-3-specific mRNA in HCT-8 cells

Erika Jensen-Jarolim, Regina Gscheidlinger, Georg Oberhuber, Czilla Neuchrist, Trevor Lucas, Giovanna Bises, Christian Radauer, Martin Willheim, Otto Scheiner, Fu-Tong Liu, George Boltz-Nitulescu

Research output: Contribution to journalArticle

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Abstract

Objective: Galectin-3, a lectin with specificity for beta galactoside, is expressed by a variety of cells, including intestinal epithelial cells. Among other functions, galectin-3 mediates cell adhesion and is involved in inflammatory processes. In this study, we assessed the expression of galectin-3 in intestinal epithelial cells from Crohn's disease patients (n = 10), ileum adjacent to resected colon carcinoma (n = 9), unspecific bowel inflammation (n = 1), diverticulosis (n = 1), ulcerative colitis (n = 3) and healthy jejunum used for interposition in larynx carcinoma (n = 1). The role of cytokines on galectin-3 expression was a further aim of our study. Methods: The galectin-3 distribution in intestinal epithelia was analysed by immunohistochemistry, immunoblotting, immunofluorescence and reverse transcriptase polymerase chain reaction (RT-PCR). Human intestinal epithelial cell line (HCT-8) and primary cultured intestinal epithelial cells were treated with cytokines, and the effects on galectin-3 expression were determined by RT-PCR. Results: Galectin-3 showed a homogeneous distribution in epithelia from control patients. In contrast, in epithelial cells from Crohn's disease lesions, galectin-3 staining was strongly spotted and heterogeneous. In inflamed and reorganized tissue, galectin-3 expression was markedly reduced, and was associated with disintegration of epithelia. Primary cultured epithelial cells as well as HCT-8 cells expressed galectin-3 protein and mRNA. Incubation of HCT-8 cells with tumour necrosis factor alpha (TNF-α), but not with other cytokines, substantially reduced galectin-3 expression as shown by semiquantitative RT-PCR. Conclusions: Downregulation of galectin-3 in the intestinal epithelium of Crohn's disease patients may be a consequence of enhanced TNF-α production by inflammatory cells, thereby contributing to the pathophysiology of the disease.

Original languageEnglish (US)
Pages (from-to)145-152
Number of pages8
JournalEuropean Journal of Gastroenterology and Hepatology
Volume14
Issue number2
DOIs
StatePublished - 2002
Externally publishedYes

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Galectin 3
Intestinal Mucosa
Crohn Disease
Down-Regulation
Tumor Necrosis Factor-alpha
Messenger RNA
Epithelial Cells
Reverse Transcriptase Polymerase Chain Reaction
Cytokines
Epithelium
Carcinoma
Diverticulum
Jejunum
Larynx
Ulcerative Colitis
Ileum
Immunoblotting
Lectins
Cell Adhesion
Fluorescent Antibody Technique

Keywords

  • Cytokines
  • Epithelial cells
  • Inflammatory bowel disease
  • Lectins

ASJC Scopus subject areas

  • Gastroenterology

Cite this

The constitutive expression of galectin-3 is downregulated in the intestinal epithelia of Crohn's disease patients, and tumour necrosis factor alpha decreases the level of galectin-3-specific mRNA in HCT-8 cells. / Jensen-Jarolim, Erika; Gscheidlinger, Regina; Oberhuber, Georg; Neuchrist, Czilla; Lucas, Trevor; Bises, Giovanna; Radauer, Christian; Willheim, Martin; Scheiner, Otto; Liu, Fu-Tong; Boltz-Nitulescu, George.

In: European Journal of Gastroenterology and Hepatology, Vol. 14, No. 2, 2002, p. 145-152.

Research output: Contribution to journalArticle

Jensen-Jarolim, Erika ; Gscheidlinger, Regina ; Oberhuber, Georg ; Neuchrist, Czilla ; Lucas, Trevor ; Bises, Giovanna ; Radauer, Christian ; Willheim, Martin ; Scheiner, Otto ; Liu, Fu-Tong ; Boltz-Nitulescu, George. / The constitutive expression of galectin-3 is downregulated in the intestinal epithelia of Crohn's disease patients, and tumour necrosis factor alpha decreases the level of galectin-3-specific mRNA in HCT-8 cells. In: European Journal of Gastroenterology and Hepatology. 2002 ; Vol. 14, No. 2. pp. 145-152.
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abstract = "Objective: Galectin-3, a lectin with specificity for beta galactoside, is expressed by a variety of cells, including intestinal epithelial cells. Among other functions, galectin-3 mediates cell adhesion and is involved in inflammatory processes. In this study, we assessed the expression of galectin-3 in intestinal epithelial cells from Crohn's disease patients (n = 10), ileum adjacent to resected colon carcinoma (n = 9), unspecific bowel inflammation (n = 1), diverticulosis (n = 1), ulcerative colitis (n = 3) and healthy jejunum used for interposition in larynx carcinoma (n = 1). The role of cytokines on galectin-3 expression was a further aim of our study. Methods: The galectin-3 distribution in intestinal epithelia was analysed by immunohistochemistry, immunoblotting, immunofluorescence and reverse transcriptase polymerase chain reaction (RT-PCR). Human intestinal epithelial cell line (HCT-8) and primary cultured intestinal epithelial cells were treated with cytokines, and the effects on galectin-3 expression were determined by RT-PCR. Results: Galectin-3 showed a homogeneous distribution in epithelia from control patients. In contrast, in epithelial cells from Crohn's disease lesions, galectin-3 staining was strongly spotted and heterogeneous. In inflamed and reorganized tissue, galectin-3 expression was markedly reduced, and was associated with disintegration of epithelia. Primary cultured epithelial cells as well as HCT-8 cells expressed galectin-3 protein and mRNA. Incubation of HCT-8 cells with tumour necrosis factor alpha (TNF-α), but not with other cytokines, substantially reduced galectin-3 expression as shown by semiquantitative RT-PCR. Conclusions: Downregulation of galectin-3 in the intestinal epithelium of Crohn's disease patients may be a consequence of enhanced TNF-α production by inflammatory cells, thereby contributing to the pathophysiology of the disease.",
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author = "Erika Jensen-Jarolim and Regina Gscheidlinger and Georg Oberhuber and Czilla Neuchrist and Trevor Lucas and Giovanna Bises and Christian Radauer and Martin Willheim and Otto Scheiner and Fu-Tong Liu and George Boltz-Nitulescu",
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T1 - The constitutive expression of galectin-3 is downregulated in the intestinal epithelia of Crohn's disease patients, and tumour necrosis factor alpha decreases the level of galectin-3-specific mRNA in HCT-8 cells

AU - Jensen-Jarolim, Erika

AU - Gscheidlinger, Regina

AU - Oberhuber, Georg

AU - Neuchrist, Czilla

AU - Lucas, Trevor

AU - Bises, Giovanna

AU - Radauer, Christian

AU - Willheim, Martin

AU - Scheiner, Otto

AU - Liu, Fu-Tong

AU - Boltz-Nitulescu, George

PY - 2002

Y1 - 2002

N2 - Objective: Galectin-3, a lectin with specificity for beta galactoside, is expressed by a variety of cells, including intestinal epithelial cells. Among other functions, galectin-3 mediates cell adhesion and is involved in inflammatory processes. In this study, we assessed the expression of galectin-3 in intestinal epithelial cells from Crohn's disease patients (n = 10), ileum adjacent to resected colon carcinoma (n = 9), unspecific bowel inflammation (n = 1), diverticulosis (n = 1), ulcerative colitis (n = 3) and healthy jejunum used for interposition in larynx carcinoma (n = 1). The role of cytokines on galectin-3 expression was a further aim of our study. Methods: The galectin-3 distribution in intestinal epithelia was analysed by immunohistochemistry, immunoblotting, immunofluorescence and reverse transcriptase polymerase chain reaction (RT-PCR). Human intestinal epithelial cell line (HCT-8) and primary cultured intestinal epithelial cells were treated with cytokines, and the effects on galectin-3 expression were determined by RT-PCR. Results: Galectin-3 showed a homogeneous distribution in epithelia from control patients. In contrast, in epithelial cells from Crohn's disease lesions, galectin-3 staining was strongly spotted and heterogeneous. In inflamed and reorganized tissue, galectin-3 expression was markedly reduced, and was associated with disintegration of epithelia. Primary cultured epithelial cells as well as HCT-8 cells expressed galectin-3 protein and mRNA. Incubation of HCT-8 cells with tumour necrosis factor alpha (TNF-α), but not with other cytokines, substantially reduced galectin-3 expression as shown by semiquantitative RT-PCR. Conclusions: Downregulation of galectin-3 in the intestinal epithelium of Crohn's disease patients may be a consequence of enhanced TNF-α production by inflammatory cells, thereby contributing to the pathophysiology of the disease.

AB - Objective: Galectin-3, a lectin with specificity for beta galactoside, is expressed by a variety of cells, including intestinal epithelial cells. Among other functions, galectin-3 mediates cell adhesion and is involved in inflammatory processes. In this study, we assessed the expression of galectin-3 in intestinal epithelial cells from Crohn's disease patients (n = 10), ileum adjacent to resected colon carcinoma (n = 9), unspecific bowel inflammation (n = 1), diverticulosis (n = 1), ulcerative colitis (n = 3) and healthy jejunum used for interposition in larynx carcinoma (n = 1). The role of cytokines on galectin-3 expression was a further aim of our study. Methods: The galectin-3 distribution in intestinal epithelia was analysed by immunohistochemistry, immunoblotting, immunofluorescence and reverse transcriptase polymerase chain reaction (RT-PCR). Human intestinal epithelial cell line (HCT-8) and primary cultured intestinal epithelial cells were treated with cytokines, and the effects on galectin-3 expression were determined by RT-PCR. Results: Galectin-3 showed a homogeneous distribution in epithelia from control patients. In contrast, in epithelial cells from Crohn's disease lesions, galectin-3 staining was strongly spotted and heterogeneous. In inflamed and reorganized tissue, galectin-3 expression was markedly reduced, and was associated with disintegration of epithelia. Primary cultured epithelial cells as well as HCT-8 cells expressed galectin-3 protein and mRNA. Incubation of HCT-8 cells with tumour necrosis factor alpha (TNF-α), but not with other cytokines, substantially reduced galectin-3 expression as shown by semiquantitative RT-PCR. Conclusions: Downregulation of galectin-3 in the intestinal epithelium of Crohn's disease patients may be a consequence of enhanced TNF-α production by inflammatory cells, thereby contributing to the pathophysiology of the disease.

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KW - Epithelial cells

KW - Inflammatory bowel disease

KW - Lectins

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