The completion of the Mammalian Gene Collection

Gary Temple, Daniela S. Gerhard, Rebekah Rasooly, Elise A. Feingold, Peter J. Good, Cristen Robinson, Allison Mandich, Jeffrey G. Derge, Jeanne Lewis, Debonny Shoaf, Francis S. Collins, Wonhee Jang, Lukas Wagner, Carolyn M. Shenmen, Leonie Misquitta, Carl F. Schaefer, Kenneth H. Buetow, Tom I. Bonner, Linda Yankie, Ming Ward & 107 others Lon Phan, Alex Astashyn, Garth Brown, Catherine Farrell, Jennifer Hart, Melissa Landrum, Bonnie L. Maidak, Michael Murphy, Terence Murphy, Bhanu Rajput, Lillian Riddick, David Webb, Janet Weber, Wendy Wu, Kim D. Pruitt, Donna Maglott, Adam Siepel, Brona Brejova, Mark Diekhans, Rachel Harte, Robert Baertsch, Jim Kent, David Haussler, Michael Brent, Laura Langton, Charles L.G. Comstock, Michael Stevens, Chaochun Wei, Marijke J. Van Baren, Kourosh Salehi-Ashtiani, Ryan R. Murray, Lila Ghamsari, Elizabeth Mello, Chenwei Lin, Christa Pennacchio, Kirsten Schreiber, Nicole Shapiro, Amber Marsh, Elizabeth Pardes, Troy Moore, Anita Lebeau, Mike Muratet, Blake Simmons, David Kloske, Stephanie Sieja, James Hudson, Praveen Sethupathy, Michael Brownstein, Narayan Bhat, Joseph Lazar, Howard Jacob, Chris E. Gruber, Mark R. Smith, John Douglas Mcpherson, Angela M. Garcia, Preethi H. Gunaratne, Jiaqian Wu, Donna Muzny, Richard A. Gibbs, Alice C. Young, Gerard G. Bouffard, Robert W. Blakesley, Jim Mullikin, Eric D. Green, Mark C. Dickson, Alex C. Rodriguez, Jane Grimwood, Jeremy Schmutz, Richard M. Myers, Martin Hirst, Thomas Zeng, Kane Tse, Michelle Moksa, Merinda Deng, Kevin Ma, Diana Mah, Johnson Pang, Greg Taylor, Eric Chuah, Athena Deng, Keith Fichter, Anne Go, Stephanie Lee, Jing Wang, Malachi Griffith, Ryan Morin, Richard A. Moore, Michael Mayo, Sarah Munro, Susan Wagner, Steven J.M. Jones, Robert A. Holt, Marco A. Marra, Sun Lu, Shuwei Yang, James Hartigan, Marcus Graf, Ralf Wagner, Stanley Letovksy, Jacqueline C. Pulido, Keith Robison, Dominic Esposito, James Hartley, Vanessa E. Wall, Ralph F. Hopkins, Osamu Ohara, Stefan Wiemann

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Since its start, the Mammalian Gene Collection (MGC) has sought to provide at least one full-protein-coding sequence cDNA clone for every human and mouse gene with a RefSeq transcript, and at least 6200 rat genes. The MGC cloning effort initially relied on random expressed sequence tag screening of cDNA libraries. Here, we summarize our recent progress using directed RT-PCR cloning and DNA synthesis. The MGC now contains clones with the entire protein-coding sequence for 92% of human and 89% of mouse genes with curated RefSeq (NM-accession) transcripts, and for 97% of human and 96% of mouse genes with curated RefSeq transcripts that have one or more PubMed publications, in addition to clones for more than 6300 rat genes. These high-quality MGC clones and their sequences are accessible without restriction to researchers worldwide.

Original languageEnglish (US)
Pages (from-to)2324-2333
Number of pages10
JournalGenome Research
Volume19
Issue number12
DOIs
StatePublished - Dec 2009
Externally publishedYes

Fingerprint

Genes
Clone Cells
Organism Cloning
Expressed Sequence Tags
Gene Library
PubMed
Publications
Proteins
Complementary DNA
Research Personnel
Polymerase Chain Reaction
DNA

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Temple, G., Gerhard, D. S., Rasooly, R., Feingold, E. A., Good, P. J., Robinson, C., ... Wiemann, S. (2009). The completion of the Mammalian Gene Collection. Genome Research, 19(12), 2324-2333. https://doi.org/10.1101/gr.095976.109

The completion of the Mammalian Gene Collection. / Temple, Gary; Gerhard, Daniela S.; Rasooly, Rebekah; Feingold, Elise A.; Good, Peter J.; Robinson, Cristen; Mandich, Allison; Derge, Jeffrey G.; Lewis, Jeanne; Shoaf, Debonny; Collins, Francis S.; Jang, Wonhee; Wagner, Lukas; Shenmen, Carolyn M.; Misquitta, Leonie; Schaefer, Carl F.; Buetow, Kenneth H.; Bonner, Tom I.; Yankie, Linda; Ward, Ming; Phan, Lon; Astashyn, Alex; Brown, Garth; Farrell, Catherine; Hart, Jennifer; Landrum, Melissa; Maidak, Bonnie L.; Murphy, Michael; Murphy, Terence; Rajput, Bhanu; Riddick, Lillian; Webb, David; Weber, Janet; Wu, Wendy; Pruitt, Kim D.; Maglott, Donna; Siepel, Adam; Brejova, Brona; Diekhans, Mark; Harte, Rachel; Baertsch, Robert; Kent, Jim; Haussler, David; Brent, Michael; Langton, Laura; Comstock, Charles L.G.; Stevens, Michael; Wei, Chaochun; Van Baren, Marijke J.; Salehi-Ashtiani, Kourosh; Murray, Ryan R.; Ghamsari, Lila; Mello, Elizabeth; Lin, Chenwei; Pennacchio, Christa; Schreiber, Kirsten; Shapiro, Nicole; Marsh, Amber; Pardes, Elizabeth; Moore, Troy; Lebeau, Anita; Muratet, Mike; Simmons, Blake; Kloske, David; Sieja, Stephanie; Hudson, James; Sethupathy, Praveen; Brownstein, Michael; Bhat, Narayan; Lazar, Joseph; Jacob, Howard; Gruber, Chris E.; Smith, Mark R.; Mcpherson, John Douglas; Garcia, Angela M.; Gunaratne, Preethi H.; Wu, Jiaqian; Muzny, Donna; Gibbs, Richard A.; Young, Alice C.; Bouffard, Gerard G.; Blakesley, Robert W.; Mullikin, Jim; Green, Eric D.; Dickson, Mark C.; Rodriguez, Alex C.; Grimwood, Jane; Schmutz, Jeremy; Myers, Richard M.; Hirst, Martin; Zeng, Thomas; Tse, Kane; Moksa, Michelle; Deng, Merinda; Ma, Kevin; Mah, Diana; Pang, Johnson; Taylor, Greg; Chuah, Eric; Deng, Athena; Fichter, Keith; Go, Anne; Lee, Stephanie; Wang, Jing; Griffith, Malachi; Morin, Ryan; Moore, Richard A.; Mayo, Michael; Munro, Sarah; Wagner, Susan; Jones, Steven J.M.; Holt, Robert A.; Marra, Marco A.; Lu, Sun; Yang, Shuwei; Hartigan, James; Graf, Marcus; Wagner, Ralf; Letovksy, Stanley; Pulido, Jacqueline C.; Robison, Keith; Esposito, Dominic; Hartley, James; Wall, Vanessa E.; Hopkins, Ralph F.; Ohara, Osamu; Wiemann, Stefan.

In: Genome Research, Vol. 19, No. 12, 12.2009, p. 2324-2333.

Research output: Contribution to journalArticle

Temple, G, Gerhard, DS, Rasooly, R, Feingold, EA, Good, PJ, Robinson, C, Mandich, A, Derge, JG, Lewis, J, Shoaf, D, Collins, FS, Jang, W, Wagner, L, Shenmen, CM, Misquitta, L, Schaefer, CF, Buetow, KH, Bonner, TI, Yankie, L, Ward, M, Phan, L, Astashyn, A, Brown, G, Farrell, C, Hart, J, Landrum, M, Maidak, BL, Murphy, M, Murphy, T, Rajput, B, Riddick, L, Webb, D, Weber, J, Wu, W, Pruitt, KD, Maglott, D, Siepel, A, Brejova, B, Diekhans, M, Harte, R, Baertsch, R, Kent, J, Haussler, D, Brent, M, Langton, L, Comstock, CLG, Stevens, M, Wei, C, Van Baren, MJ, Salehi-Ashtiani, K, Murray, RR, Ghamsari, L, Mello, E, Lin, C, Pennacchio, C, Schreiber, K, Shapiro, N, Marsh, A, Pardes, E, Moore, T, Lebeau, A, Muratet, M, Simmons, B, Kloske, D, Sieja, S, Hudson, J, Sethupathy, P, Brownstein, M, Bhat, N, Lazar, J, Jacob, H, Gruber, CE, Smith, MR, Mcpherson, JD, Garcia, AM, Gunaratne, PH, Wu, J, Muzny, D, Gibbs, RA, Young, AC, Bouffard, GG, Blakesley, RW, Mullikin, J, Green, ED, Dickson, MC, Rodriguez, AC, Grimwood, J, Schmutz, J, Myers, RM, Hirst, M, Zeng, T, Tse, K, Moksa, M, Deng, M, Ma, K, Mah, D, Pang, J, Taylor, G, Chuah, E, Deng, A, Fichter, K, Go, A, Lee, S, Wang, J, Griffith, M, Morin, R, Moore, RA, Mayo, M, Munro, S, Wagner, S, Jones, SJM, Holt, RA, Marra, MA, Lu, S, Yang, S, Hartigan, J, Graf, M, Wagner, R, Letovksy, S, Pulido, JC, Robison, K, Esposito, D, Hartley, J, Wall, VE, Hopkins, RF, Ohara, O & Wiemann, S 2009, 'The completion of the Mammalian Gene Collection', Genome Research, vol. 19, no. 12, pp. 2324-2333. https://doi.org/10.1101/gr.095976.109
Temple G, Gerhard DS, Rasooly R, Feingold EA, Good PJ, Robinson C et al. The completion of the Mammalian Gene Collection. Genome Research. 2009 Dec;19(12):2324-2333. https://doi.org/10.1101/gr.095976.109
Temple, Gary ; Gerhard, Daniela S. ; Rasooly, Rebekah ; Feingold, Elise A. ; Good, Peter J. ; Robinson, Cristen ; Mandich, Allison ; Derge, Jeffrey G. ; Lewis, Jeanne ; Shoaf, Debonny ; Collins, Francis S. ; Jang, Wonhee ; Wagner, Lukas ; Shenmen, Carolyn M. ; Misquitta, Leonie ; Schaefer, Carl F. ; Buetow, Kenneth H. ; Bonner, Tom I. ; Yankie, Linda ; Ward, Ming ; Phan, Lon ; Astashyn, Alex ; Brown, Garth ; Farrell, Catherine ; Hart, Jennifer ; Landrum, Melissa ; Maidak, Bonnie L. ; Murphy, Michael ; Murphy, Terence ; Rajput, Bhanu ; Riddick, Lillian ; Webb, David ; Weber, Janet ; Wu, Wendy ; Pruitt, Kim D. ; Maglott, Donna ; Siepel, Adam ; Brejova, Brona ; Diekhans, Mark ; Harte, Rachel ; Baertsch, Robert ; Kent, Jim ; Haussler, David ; Brent, Michael ; Langton, Laura ; Comstock, Charles L.G. ; Stevens, Michael ; Wei, Chaochun ; Van Baren, Marijke J. ; Salehi-Ashtiani, Kourosh ; Murray, Ryan R. ; Ghamsari, Lila ; Mello, Elizabeth ; Lin, Chenwei ; Pennacchio, Christa ; Schreiber, Kirsten ; Shapiro, Nicole ; Marsh, Amber ; Pardes, Elizabeth ; Moore, Troy ; Lebeau, Anita ; Muratet, Mike ; Simmons, Blake ; Kloske, David ; Sieja, Stephanie ; Hudson, James ; Sethupathy, Praveen ; Brownstein, Michael ; Bhat, Narayan ; Lazar, Joseph ; Jacob, Howard ; Gruber, Chris E. ; Smith, Mark R. ; Mcpherson, John Douglas ; Garcia, Angela M. ; Gunaratne, Preethi H. ; Wu, Jiaqian ; Muzny, Donna ; Gibbs, Richard A. ; Young, Alice C. ; Bouffard, Gerard G. ; Blakesley, Robert W. ; Mullikin, Jim ; Green, Eric D. ; Dickson, Mark C. ; Rodriguez, Alex C. ; Grimwood, Jane ; Schmutz, Jeremy ; Myers, Richard M. ; Hirst, Martin ; Zeng, Thomas ; Tse, Kane ; Moksa, Michelle ; Deng, Merinda ; Ma, Kevin ; Mah, Diana ; Pang, Johnson ; Taylor, Greg ; Chuah, Eric ; Deng, Athena ; Fichter, Keith ; Go, Anne ; Lee, Stephanie ; Wang, Jing ; Griffith, Malachi ; Morin, Ryan ; Moore, Richard A. ; Mayo, Michael ; Munro, Sarah ; Wagner, Susan ; Jones, Steven J.M. ; Holt, Robert A. ; Marra, Marco A. ; Lu, Sun ; Yang, Shuwei ; Hartigan, James ; Graf, Marcus ; Wagner, Ralf ; Letovksy, Stanley ; Pulido, Jacqueline C. ; Robison, Keith ; Esposito, Dominic ; Hartley, James ; Wall, Vanessa E. ; Hopkins, Ralph F. ; Ohara, Osamu ; Wiemann, Stefan. / The completion of the Mammalian Gene Collection. In: Genome Research. 2009 ; Vol. 19, No. 12. pp. 2324-2333.
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AU - Mandich, Allison

AU - Derge, Jeffrey G.

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AU - Maglott, Donna

AU - Siepel, Adam

AU - Brejova, Brona

AU - Diekhans, Mark

AU - Harte, Rachel

AU - Baertsch, Robert

AU - Kent, Jim

AU - Haussler, David

AU - Brent, Michael

AU - Langton, Laura

AU - Comstock, Charles L.G.

AU - Stevens, Michael

AU - Wei, Chaochun

AU - Van Baren, Marijke J.

AU - Salehi-Ashtiani, Kourosh

AU - Murray, Ryan R.

AU - Ghamsari, Lila

AU - Mello, Elizabeth

AU - Lin, Chenwei

AU - Pennacchio, Christa

AU - Schreiber, Kirsten

AU - Shapiro, Nicole

AU - Marsh, Amber

AU - Pardes, Elizabeth

AU - Moore, Troy

AU - Lebeau, Anita

AU - Muratet, Mike

AU - Simmons, Blake

AU - Kloske, David

AU - Sieja, Stephanie

AU - Hudson, James

AU - Sethupathy, Praveen

AU - Brownstein, Michael

AU - Bhat, Narayan

AU - Lazar, Joseph

AU - Jacob, Howard

AU - Gruber, Chris E.

AU - Smith, Mark R.

AU - Mcpherson, John Douglas

AU - Garcia, Angela M.

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AU - Muzny, Donna

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AU - Young, Alice C.

AU - Bouffard, Gerard G.

AU - Blakesley, Robert W.

AU - Mullikin, Jim

AU - Green, Eric D.

AU - Dickson, Mark C.

AU - Rodriguez, Alex C.

AU - Grimwood, Jane

AU - Schmutz, Jeremy

AU - Myers, Richard M.

AU - Hirst, Martin

AU - Zeng, Thomas

AU - Tse, Kane

AU - Moksa, Michelle

AU - Deng, Merinda

AU - Ma, Kevin

AU - Mah, Diana

AU - Pang, Johnson

AU - Taylor, Greg

AU - Chuah, Eric

AU - Deng, Athena

AU - Fichter, Keith

AU - Go, Anne

AU - Lee, Stephanie

AU - Wang, Jing

AU - Griffith, Malachi

AU - Morin, Ryan

AU - Moore, Richard A.

AU - Mayo, Michael

AU - Munro, Sarah

AU - Wagner, Susan

AU - Jones, Steven J.M.

AU - Holt, Robert A.

AU - Marra, Marco A.

AU - Lu, Sun

AU - Yang, Shuwei

AU - Hartigan, James

AU - Graf, Marcus

AU - Wagner, Ralf

AU - Letovksy, Stanley

AU - Pulido, Jacqueline C.

AU - Robison, Keith

AU - Esposito, Dominic

AU - Hartley, James

AU - Wall, Vanessa E.

AU - Hopkins, Ralph F.

AU - Ohara, Osamu

AU - Wiemann, Stefan

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