The complete genome sequence of Haloferax volcanii DS2, a model archaeon

Amber L. Hartman, Cédric Norais, Jonathan H. Badger, Stéphane Delmas, Sam Haldenby, Ramana Madupu, Jeffrey Robinson, Hoda Khouri, Qinghu Ren, Todd M. Lowe, Julie Maupin-Furlow, Mecky Pohlschroder, Charles Daniels, Friedhelm Pfeiffer, Thorsten Allers, Jonathan A Eisen

Research output: Contribution to journalArticle

167 Citations (Scopus)

Abstract

Background: Haloferax volcanii is an easily culturable moderate halophile that grows on simple defined media, is readily transformable, and has a relatively stable genome. This, in combination with its biochemical and genetic tractability, has made Hfx. volcanii a key model organism, not only for the study of halophilicity, but also for archaeal biology in general. Methodology/Principal Findings: We report here the sequencing and analysis of the genome of Hfx. volcanii DS2, the type strain of this species. The genome contains a main 2.848 Mb chromosome, three smaller chromosomes pHV1, 3, 4 (85, 438, 636 kb, respectively) and the pHV2 plasmid (6.4 kb). Conclusions/Significance: The completed genome sequence, presented here, provides an invaluable tool for further in vivo and in vitro studies of Hfx. volcanii.

Original languageEnglish (US)
Article numbere9605
JournalPLoS One
Volume5
Issue number3
DOIs
StatePublished - 2010

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Haloferax volcanii
Archaea
Genes
Genome
genome
Chromosomes
chromosomes
Chromosomes, Human, Pair 3
in vitro studies
Molecular Biology
plasmids
Plasmids
Biological Sciences
organisms

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Hartman, A. L., Norais, C., Badger, J. H., Delmas, S., Haldenby, S., Madupu, R., ... Eisen, J. A. (2010). The complete genome sequence of Haloferax volcanii DS2, a model archaeon. PLoS One, 5(3), [e9605]. https://doi.org/10.1371/journal.pone.0009605

The complete genome sequence of Haloferax volcanii DS2, a model archaeon. / Hartman, Amber L.; Norais, Cédric; Badger, Jonathan H.; Delmas, Stéphane; Haldenby, Sam; Madupu, Ramana; Robinson, Jeffrey; Khouri, Hoda; Ren, Qinghu; Lowe, Todd M.; Maupin-Furlow, Julie; Pohlschroder, Mecky; Daniels, Charles; Pfeiffer, Friedhelm; Allers, Thorsten; Eisen, Jonathan A.

In: PLoS One, Vol. 5, No. 3, e9605, 2010.

Research output: Contribution to journalArticle

Hartman, AL, Norais, C, Badger, JH, Delmas, S, Haldenby, S, Madupu, R, Robinson, J, Khouri, H, Ren, Q, Lowe, TM, Maupin-Furlow, J, Pohlschroder, M, Daniels, C, Pfeiffer, F, Allers, T & Eisen, JA 2010, 'The complete genome sequence of Haloferax volcanii DS2, a model archaeon', PLoS One, vol. 5, no. 3, e9605. https://doi.org/10.1371/journal.pone.0009605
Hartman AL, Norais C, Badger JH, Delmas S, Haldenby S, Madupu R et al. The complete genome sequence of Haloferax volcanii DS2, a model archaeon. PLoS One. 2010;5(3). e9605. https://doi.org/10.1371/journal.pone.0009605
Hartman, Amber L. ; Norais, Cédric ; Badger, Jonathan H. ; Delmas, Stéphane ; Haldenby, Sam ; Madupu, Ramana ; Robinson, Jeffrey ; Khouri, Hoda ; Ren, Qinghu ; Lowe, Todd M. ; Maupin-Furlow, Julie ; Pohlschroder, Mecky ; Daniels, Charles ; Pfeiffer, Friedhelm ; Allers, Thorsten ; Eisen, Jonathan A. / The complete genome sequence of Haloferax volcanii DS2, a model archaeon. In: PLoS One. 2010 ; Vol. 5, No. 3.
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