The complete genome of Teredinibacter turnerae T7901: An intracellular endosymbiont of marine wood-boring bivalves (shipworms)

Joyce C. Yang, Ramana Madupu, A. Scott Durkin, Nathan A. Ekborg, Chandra S. Pedamallu, Jessica B. Hostetler, Diana Radune, Bradley S. Toms, Bernard Henrissat, Pedro M. Coutinho, Sandra Schwarz, Lauren Field, Amaro E. Trindade-Silva, Carlos A G Soares, Sherif Elshahawi, Amro Hanora, Eric W. Schmidt, Margo G. Haygood, Janos Posfai, Jack BennerCatherine Madinger, John Nove, Brian Anton, Kshitiz Chaudhary, Jeremy Foster, Alex Holman, Sanjay Kumar, Philip A. Lessard, Yvette A. Luyten, Barton Slatko, Nicole Wood, Bo Wu, Max Teplitski, Joseph D. Mougous, Naomi Ward, Jonathan A Eisen, Jonathan H. Badger, Daniel L. Distel

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Here we report the complete genome sequence of Teredinibacter turnerae T7901. T. turnerae is a marine gamma proteobacterium that occurs as an intracellular endosymbiont in the gills of wood-boring marine bivalves of the family Teredinidae (shipworms). This species is the sole cultivated member of an endosymbiotic consortium thought to provide the host with enzymes, including cellulases and nitrogenase, critical for digestion of wood and supplementation of the host's nitrogen-deficient diet. T. turnerae is closely related to the free-living marine polysaccharide degrading bacterium Saccharophagus degradans str. 2-40 and to as yet uncultivated endosymbionts with which it coexists in shipworm cells. Like S. degradans, the T. turnerae genome encodes a large number of enzymes predicted to be involved in complex polysaccharide degradation (>100). However, unlike S. degradans, which degrades a broad spectrum (>10 classes) of complex plant, fungal and algal polysaccharides, T. turnerae primarily encodes enzymes associated with deconstruction of terrestrial woody plant material. Also unlike S. degradans and many other eubacteria, T. turnerae dedicates a large proportion of its genome to genes predicted to function in secondary metabolism. Despite its intracellular niche, the T. turnerae genome lacks many features associated with obligate intracellular existence (e.g. reduced genome size, reduced %G+C, loss of genes of core metabolism) and displays evidence of adaptations common to free-living bacteria (e.g. defense against bacteriophage infection). These results suggest that T. turnerae is likely a facultative intracellular ensosymbiont whose niche presently includes, or recently included, free-living existence. As such, the T. turnerae genome provides insights into the range of genomic adaptations associated with intracellular endosymbiosis as well as enzymatic mechanisms relevant to the recycling of plant materials in marine environments and the production of cellulose-derived biofuels.

Original languageEnglish (US)
Article numbere6085
JournalPLoS One
Volume4
Issue number7
DOIs
StatePublished - Jul 1 2009

Fingerprint

Teredinibacter turnerae
Boring
Bivalvia
endosymbionts
Wood
Genes
Genome
genome
Bacteria
Polysaccharides
Fungal Polysaccharides
Enzymes
Secondary Metabolism
Gammaproteobacteria
polysaccharides
Genome Size
Cellulases
Nitrogenase
Symbiosis
Biofuels

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Yang, J. C., Madupu, R., Durkin, A. S., Ekborg, N. A., Pedamallu, C. S., Hostetler, J. B., ... Distel, D. L. (2009). The complete genome of Teredinibacter turnerae T7901: An intracellular endosymbiont of marine wood-boring bivalves (shipworms). PLoS One, 4(7), [e6085]. https://doi.org/10.1371/journal.pone.0006085

The complete genome of Teredinibacter turnerae T7901 : An intracellular endosymbiont of marine wood-boring bivalves (shipworms). / Yang, Joyce C.; Madupu, Ramana; Durkin, A. Scott; Ekborg, Nathan A.; Pedamallu, Chandra S.; Hostetler, Jessica B.; Radune, Diana; Toms, Bradley S.; Henrissat, Bernard; Coutinho, Pedro M.; Schwarz, Sandra; Field, Lauren; Trindade-Silva, Amaro E.; Soares, Carlos A G; Elshahawi, Sherif; Hanora, Amro; Schmidt, Eric W.; Haygood, Margo G.; Posfai, Janos; Benner, Jack; Madinger, Catherine; Nove, John; Anton, Brian; Chaudhary, Kshitiz; Foster, Jeremy; Holman, Alex; Kumar, Sanjay; Lessard, Philip A.; Luyten, Yvette A.; Slatko, Barton; Wood, Nicole; Wu, Bo; Teplitski, Max; Mougous, Joseph D.; Ward, Naomi; Eisen, Jonathan A; Badger, Jonathan H.; Distel, Daniel L.

In: PLoS One, Vol. 4, No. 7, e6085, 01.07.2009.

Research output: Contribution to journalArticle

Yang, JC, Madupu, R, Durkin, AS, Ekborg, NA, Pedamallu, CS, Hostetler, JB, Radune, D, Toms, BS, Henrissat, B, Coutinho, PM, Schwarz, S, Field, L, Trindade-Silva, AE, Soares, CAG, Elshahawi, S, Hanora, A, Schmidt, EW, Haygood, MG, Posfai, J, Benner, J, Madinger, C, Nove, J, Anton, B, Chaudhary, K, Foster, J, Holman, A, Kumar, S, Lessard, PA, Luyten, YA, Slatko, B, Wood, N, Wu, B, Teplitski, M, Mougous, JD, Ward, N, Eisen, JA, Badger, JH & Distel, DL 2009, 'The complete genome of Teredinibacter turnerae T7901: An intracellular endosymbiont of marine wood-boring bivalves (shipworms)', PLoS One, vol. 4, no. 7, e6085. https://doi.org/10.1371/journal.pone.0006085
Yang, Joyce C. ; Madupu, Ramana ; Durkin, A. Scott ; Ekborg, Nathan A. ; Pedamallu, Chandra S. ; Hostetler, Jessica B. ; Radune, Diana ; Toms, Bradley S. ; Henrissat, Bernard ; Coutinho, Pedro M. ; Schwarz, Sandra ; Field, Lauren ; Trindade-Silva, Amaro E. ; Soares, Carlos A G ; Elshahawi, Sherif ; Hanora, Amro ; Schmidt, Eric W. ; Haygood, Margo G. ; Posfai, Janos ; Benner, Jack ; Madinger, Catherine ; Nove, John ; Anton, Brian ; Chaudhary, Kshitiz ; Foster, Jeremy ; Holman, Alex ; Kumar, Sanjay ; Lessard, Philip A. ; Luyten, Yvette A. ; Slatko, Barton ; Wood, Nicole ; Wu, Bo ; Teplitski, Max ; Mougous, Joseph D. ; Ward, Naomi ; Eisen, Jonathan A ; Badger, Jonathan H. ; Distel, Daniel L. / The complete genome of Teredinibacter turnerae T7901 : An intracellular endosymbiont of marine wood-boring bivalves (shipworms). In: PLoS One. 2009 ; Vol. 4, No. 7.
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abstract = "Here we report the complete genome sequence of Teredinibacter turnerae T7901. T. turnerae is a marine gamma proteobacterium that occurs as an intracellular endosymbiont in the gills of wood-boring marine bivalves of the family Teredinidae (shipworms). This species is the sole cultivated member of an endosymbiotic consortium thought to provide the host with enzymes, including cellulases and nitrogenase, critical for digestion of wood and supplementation of the host's nitrogen-deficient diet. T. turnerae is closely related to the free-living marine polysaccharide degrading bacterium Saccharophagus degradans str. 2-40 and to as yet uncultivated endosymbionts with which it coexists in shipworm cells. Like S. degradans, the T. turnerae genome encodes a large number of enzymes predicted to be involved in complex polysaccharide degradation (>100). However, unlike S. degradans, which degrades a broad spectrum (>10 classes) of complex plant, fungal and algal polysaccharides, T. turnerae primarily encodes enzymes associated with deconstruction of terrestrial woody plant material. Also unlike S. degradans and many other eubacteria, T. turnerae dedicates a large proportion of its genome to genes predicted to function in secondary metabolism. Despite its intracellular niche, the T. turnerae genome lacks many features associated with obligate intracellular existence (e.g. reduced genome size, reduced {\%}G+C, loss of genes of core metabolism) and displays evidence of adaptations common to free-living bacteria (e.g. defense against bacteriophage infection). These results suggest that T. turnerae is likely a facultative intracellular ensosymbiont whose niche presently includes, or recently included, free-living existence. As such, the T. turnerae genome provides insights into the range of genomic adaptations associated with intracellular endosymbiosis as well as enzymatic mechanisms relevant to the recycling of plant materials in marine environments and the production of cellulose-derived biofuels.",
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