The combined effect of interferon beta and radiation on five human tumor cell lines and embryonal lung fibroblasts

Heinz Schmidberger, Margret Rave-Fr̈ank, J̈org Lehmann, Simone Schweinfurth, Erika Rehring, Karin Henckel, Clemens F. Hess

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Purpose: The combined effect of natural Interferon-beta (n-IFN-β) and ionizing radiation was tested in vitro on 5 different tumor cell lines and 1 embryonal lung fibroblast cell line. Materials and Methods: The following cell lines were used: A549 (lung cancer), MCF-7 (breast cancer), CaSki (cervical cancer), WiDr (colon cancer), ZMK-1 (head and neck cancer), and MRC-5 (embryohal lung fibroblast line). Cells were incubated with n-IFN-β (30 I.U./ml to 3000 l.U./ml) 24 h before irradiation. Irradiation was given as single dose between 1 and 6 Gy. Cell survival was evaluated using a standard colony-forming assay. Results: Incubation with n-IFN-β enhanced the effect of radiation in all tumor cell lines tested. The maximum sensitizing enhancement ratios (SER) at the 37% survival level were: 1.66 for A549 cells, 1.47 for CaSki cells, 1.56 for MCF-7 cells, 1.40 for WiDr cells, and 1.57 for ZMK-1 cells. In the nonneoplastic MRC-5 cell line, no radiosensitizing effect of n-IFN-β could be demonstrated. The linear quadratic fit of the survival curves showed an increase of the α-component for all tumor cell lines treated with n-IFN-β. Conclusions: IFN-β enhanced the effect of radiation in the tumor cell lines, but not in the nonmalignant lung fibroblasts. The increase of the α component in the survival curves indicates that impaired radiation repair or the accumulation of sublethal damage might play a role for the radiosensitizing effect of n-IFN-β.

Original languageEnglish (US)
Pages (from-to)405-412
Number of pages8
JournalInternational Journal of Radiation Oncology Biology Physics
Volume43
Issue number2
DOIs
StatePublished - Jan 15 1999
Externally publishedYes

Fingerprint

interferon
Beta Particles
Interferon-beta
fibroblasts
Tumor Cell Line
cultured cells
lungs
tumors
Fibroblasts
Lung
cancer
radiation
Radiation-Sensitizing Agents
cells
Radiation Effects
Cell Line
Colonic Neoplasms
Survival
MCF-7 Cells
sensitizing

Keywords

  • Cell lines
  • Cytokines
  • In vitro
  • Interferon
  • Radiation
  • Radiosensitization

ASJC Scopus subject areas

  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Radiation

Cite this

The combined effect of interferon beta and radiation on five human tumor cell lines and embryonal lung fibroblasts. / Schmidberger, Heinz; Rave-Fr̈ank, Margret; Lehmann, J̈org; Schweinfurth, Simone; Rehring, Erika; Henckel, Karin; Hess, Clemens F.

In: International Journal of Radiation Oncology Biology Physics, Vol. 43, No. 2, 15.01.1999, p. 405-412.

Research output: Contribution to journalArticle

Schmidberger, Heinz ; Rave-Fr̈ank, Margret ; Lehmann, J̈org ; Schweinfurth, Simone ; Rehring, Erika ; Henckel, Karin ; Hess, Clemens F. / The combined effect of interferon beta and radiation on five human tumor cell lines and embryonal lung fibroblasts. In: International Journal of Radiation Oncology Biology Physics. 1999 ; Vol. 43, No. 2. pp. 405-412.
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abstract = "Purpose: The combined effect of natural Interferon-beta (n-IFN-β) and ionizing radiation was tested in vitro on 5 different tumor cell lines and 1 embryonal lung fibroblast cell line. Materials and Methods: The following cell lines were used: A549 (lung cancer), MCF-7 (breast cancer), CaSki (cervical cancer), WiDr (colon cancer), ZMK-1 (head and neck cancer), and MRC-5 (embryohal lung fibroblast line). Cells were incubated with n-IFN-β (30 I.U./ml to 3000 l.U./ml) 24 h before irradiation. Irradiation was given as single dose between 1 and 6 Gy. Cell survival was evaluated using a standard colony-forming assay. Results: Incubation with n-IFN-β enhanced the effect of radiation in all tumor cell lines tested. The maximum sensitizing enhancement ratios (SER) at the 37{\%} survival level were: 1.66 for A549 cells, 1.47 for CaSki cells, 1.56 for MCF-7 cells, 1.40 for WiDr cells, and 1.57 for ZMK-1 cells. In the nonneoplastic MRC-5 cell line, no radiosensitizing effect of n-IFN-β could be demonstrated. The linear quadratic fit of the survival curves showed an increase of the α-component for all tumor cell lines treated with n-IFN-β. Conclusions: IFN-β enhanced the effect of radiation in the tumor cell lines, but not in the nonmalignant lung fibroblasts. The increase of the α component in the survival curves indicates that impaired radiation repair or the accumulation of sublethal damage might play a role for the radiosensitizing effect of n-IFN-β.",
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T1 - The combined effect of interferon beta and radiation on five human tumor cell lines and embryonal lung fibroblasts

AU - Schmidberger, Heinz

AU - Rave-Fr̈ank, Margret

AU - Lehmann, J̈org

AU - Schweinfurth, Simone

AU - Rehring, Erika

AU - Henckel, Karin

AU - Hess, Clemens F.

PY - 1999/1/15

Y1 - 1999/1/15

N2 - Purpose: The combined effect of natural Interferon-beta (n-IFN-β) and ionizing radiation was tested in vitro on 5 different tumor cell lines and 1 embryonal lung fibroblast cell line. Materials and Methods: The following cell lines were used: A549 (lung cancer), MCF-7 (breast cancer), CaSki (cervical cancer), WiDr (colon cancer), ZMK-1 (head and neck cancer), and MRC-5 (embryohal lung fibroblast line). Cells were incubated with n-IFN-β (30 I.U./ml to 3000 l.U./ml) 24 h before irradiation. Irradiation was given as single dose between 1 and 6 Gy. Cell survival was evaluated using a standard colony-forming assay. Results: Incubation with n-IFN-β enhanced the effect of radiation in all tumor cell lines tested. The maximum sensitizing enhancement ratios (SER) at the 37% survival level were: 1.66 for A549 cells, 1.47 for CaSki cells, 1.56 for MCF-7 cells, 1.40 for WiDr cells, and 1.57 for ZMK-1 cells. In the nonneoplastic MRC-5 cell line, no radiosensitizing effect of n-IFN-β could be demonstrated. The linear quadratic fit of the survival curves showed an increase of the α-component for all tumor cell lines treated with n-IFN-β. Conclusions: IFN-β enhanced the effect of radiation in the tumor cell lines, but not in the nonmalignant lung fibroblasts. The increase of the α component in the survival curves indicates that impaired radiation repair or the accumulation of sublethal damage might play a role for the radiosensitizing effect of n-IFN-β.

AB - Purpose: The combined effect of natural Interferon-beta (n-IFN-β) and ionizing radiation was tested in vitro on 5 different tumor cell lines and 1 embryonal lung fibroblast cell line. Materials and Methods: The following cell lines were used: A549 (lung cancer), MCF-7 (breast cancer), CaSki (cervical cancer), WiDr (colon cancer), ZMK-1 (head and neck cancer), and MRC-5 (embryohal lung fibroblast line). Cells were incubated with n-IFN-β (30 I.U./ml to 3000 l.U./ml) 24 h before irradiation. Irradiation was given as single dose between 1 and 6 Gy. Cell survival was evaluated using a standard colony-forming assay. Results: Incubation with n-IFN-β enhanced the effect of radiation in all tumor cell lines tested. The maximum sensitizing enhancement ratios (SER) at the 37% survival level were: 1.66 for A549 cells, 1.47 for CaSki cells, 1.56 for MCF-7 cells, 1.40 for WiDr cells, and 1.57 for ZMK-1 cells. In the nonneoplastic MRC-5 cell line, no radiosensitizing effect of n-IFN-β could be demonstrated. The linear quadratic fit of the survival curves showed an increase of the α-component for all tumor cell lines treated with n-IFN-β. Conclusions: IFN-β enhanced the effect of radiation in the tumor cell lines, but not in the nonmalignant lung fibroblasts. The increase of the α component in the survival curves indicates that impaired radiation repair or the accumulation of sublethal damage might play a role for the radiosensitizing effect of n-IFN-β.

KW - Cell lines

KW - Cytokines

KW - In vitro

KW - Interferon

KW - Radiation

KW - Radiosensitization

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