The collagen gene: Evidence for its evolutionary assembly by amplification of a DNA segment containing an exon of 54 bp

Yoshihiko Yamada, V. Enrico Avvedimento, Maria Mudryj, Hiroaki Ohkubo, Gabriel Vogeli, Meher Irani, Ira Pastan, Benoit de Crombrugghe

Research output: Contribution to journalArticle

120 Scopus citations

Abstract

We have determined the size and sequence of 8 exons in the gene that specifies chick type I α2 collagen. These 8 exons represent three different segments of the gene, but all encode information for the helical portion of the protein. Seven exons have a length of 54 bp, and the 8th has a size of 99 bp. The sequences within these exons vary except for the glycine codons, which occur every third triplet. Each exon begins with a glycine codon and ends with a triplet that precedes a glycine codon. The size and the sequences of the introns do not show any homology except at their ends. Of 7 introns examined the first six bases at the 5′ end of 5 of these are identical. The sequences at the 3′ end of the introns also show homologies. Our results imply that the ancestral gene for collagen arose by multiple duplications of a single genetic unit containing a 54 bp coding segment. The sequences within these exons drifted by successive point mutations and in some cases by additions or deletions of 9 bp or multiples of 9 bp.

Original languageEnglish (US)
Pages (from-to)887-892
Number of pages6
JournalCell
Volume22
Issue number3
DOIs
StatePublished - 1980
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

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    Yamada, Y., Enrico Avvedimento, V., Mudryj, M., Ohkubo, H., Vogeli, G., Irani, M., Pastan, I., & de Crombrugghe, B. (1980). The collagen gene: Evidence for its evolutionary assembly by amplification of a DNA segment containing an exon of 54 bp. Cell, 22(3), 887-892. https://doi.org/10.1016/0092-8674(80)90565-6