The coexpression of CD45RA and CD45RO isoforms on T cells during the S/G2/M stages of cell cycle

Janine M LaSalle, David A. Hafler

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The tyrosine phosphatase CD45 is alternatively spliced to generate isoforms of different molecular weights (180-220 kDa) which are differentially expressed on hematopoietic cells. Monoclonal antibodies reacting with either the 180-kDa (UCHL-1, CD45RO) or the 200- to 220-kDa (2H4, CD45RA) isoform have been used to subdivide T cell populations based on their expression of one or the other of these two epitopes. CD45RA T cells have "naive" characteristics of unresponsiveness to recall antigens and prominence in cord blood, while CD45RO T cells are considered "memory" T cells because they proliferate to recall antigens and increase following PHA activation of cord blood. However, we have recently demonstrated the expression of the CD45RA isoform on a subpopulation of CD45RO+ T cell clones, suggesting that CD45RA is not a universal marker for naive T cells. Using propidium iodide staining of the DNA to determine cell cycle stage, we now show that CD45RA expression is significantly higher on T cell clones during the S, G2, and M stages of cell cycle when compared to CD45RA expression on cells in G0 and G1. Furthermore, CD45RA expression on cells undergoing mitosis is not limited to long-term activated T cell clones, as uncultured peripheral blood T cells in the S/G2/M phase express significantly more CD45RA. The percentage of T cells coexpressing CD45RA and CD45RO also increases following PHA activation, indicating that T cells in the process of division express both isoforms. These results suggest a potential role of the CD45RA isoform during the stages of cell cycle leading to mitosis.

Original languageEnglish (US)
Pages (from-to)197-206
Number of pages10
JournalCellular Immunology
Volume138
Issue number1
DOIs
StatePublished - 1991
Externally publishedYes

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Cell Cycle
Protein Isoforms
T-Lymphocytes
Clone Cells
Fetal Blood
Mitosis
Antigens
Propidium
G2 Phase
Phosphoric Monoester Hydrolases
Cell Division
Tyrosine
Epitopes
Blood Cells
Molecular Weight
Monoclonal Antibodies
Staining and Labeling
DNA
Population

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

The coexpression of CD45RA and CD45RO isoforms on T cells during the S/G2/M stages of cell cycle. / LaSalle, Janine M; Hafler, David A.

In: Cellular Immunology, Vol. 138, No. 1, 1991, p. 197-206.

Research output: Contribution to journalArticle

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