Abstract
The double-stranded (ds) RNA genome segments 2, 5, 6, and 8, which encode the outer capsid proteins P2 and P5, and the two nonstructural proteins, NS1 and NS2, respectively, of bluetongue virus (BTV) serotype 17 have been cloned into pBR322. The length of the cloned genes indicates that the entire respective dsRNA genome has been cloned in each case. The four cloned genes were used as 32P-labeled probes to hybridize to PAGE-separated United States BTV serotypes 2, 10, 11, 13, and 17. Of these, the segment 6 clone was identified as being highly conserved and hybridized to all serotypes. Segment 8 was less conserved and segment 5 even less. The segment 2 clone was serotype specific. None of the probes hybridized with dsRNA from epizootic hemorrhagic disease virus (EHDV).
| Original language | English (US) |
|---|---|
| Pages (from-to) | 296-298 |
| Number of pages | 3 |
| Journal | Virology |
| Volume | 167 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 1988 |
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ASJC Scopus subject areas
- Virology
- Infectious Diseases
Cite this
The cloning of full-length genome segments 2, 5, 6, and 8 of bluetongue virus (BTV) serotype 17 and studies of their genetic relatedness to United States BTV serotypes. / Unger, R. E.; Chuang, R. Y.; Chuang, L. F.; Osburn, Bennie; Doi, R. H.
In: Virology, Vol. 167, No. 1, 01.01.1988, p. 296-298.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The cloning of full-length genome segments 2, 5, 6, and 8 of bluetongue virus (BTV) serotype 17 and studies of their genetic relatedness to United States BTV serotypes
AU - Unger, R. E.
AU - Chuang, R. Y.
AU - Chuang, L. F.
AU - Osburn, Bennie
AU - Doi, R. H.
PY - 1988/1/1
Y1 - 1988/1/1
N2 - The double-stranded (ds) RNA genome segments 2, 5, 6, and 8, which encode the outer capsid proteins P2 and P5, and the two nonstructural proteins, NS1 and NS2, respectively, of bluetongue virus (BTV) serotype 17 have been cloned into pBR322. The length of the cloned genes indicates that the entire respective dsRNA genome has been cloned in each case. The four cloned genes were used as 32P-labeled probes to hybridize to PAGE-separated United States BTV serotypes 2, 10, 11, 13, and 17. Of these, the segment 6 clone was identified as being highly conserved and hybridized to all serotypes. Segment 8 was less conserved and segment 5 even less. The segment 2 clone was serotype specific. None of the probes hybridized with dsRNA from epizootic hemorrhagic disease virus (EHDV).
AB - The double-stranded (ds) RNA genome segments 2, 5, 6, and 8, which encode the outer capsid proteins P2 and P5, and the two nonstructural proteins, NS1 and NS2, respectively, of bluetongue virus (BTV) serotype 17 have been cloned into pBR322. The length of the cloned genes indicates that the entire respective dsRNA genome has been cloned in each case. The four cloned genes were used as 32P-labeled probes to hybridize to PAGE-separated United States BTV serotypes 2, 10, 11, 13, and 17. Of these, the segment 6 clone was identified as being highly conserved and hybridized to all serotypes. Segment 8 was less conserved and segment 5 even less. The segment 2 clone was serotype specific. None of the probes hybridized with dsRNA from epizootic hemorrhagic disease virus (EHDV).
UR - http://www.scopus.com/inward/record.url?scp=0024110392&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024110392&partnerID=8YFLogxK
U2 - 10.1016/0042-6822(88)90083-9
DO - 10.1016/0042-6822(88)90083-9
M3 - Article
C2 - 2847419
AN - SCOPUS:0024110392
VL - 167
SP - 296
EP - 298
JO - Virology
JF - Virology
SN - 0042-6822
IS - 1
ER -