Abstract
The mammalian soluble epoxide hydrolase (sEH) plays a role in the regulation of blood pressure and vascular homeostasis through its hydrolysis of the endothelial-derived messenger molecules, the epoxyeicosatrienoic acids. This study reports the cloning and expression of a sEH homolog from chicken liver. The resulting 63-kDa protein has an isoelectric point of 6.1. The recombinant enzyme displayed epoxide hydrolase activity when assayed with [ 3H]-trans-1,3-diphenylpropene oxide (t-DPPO), as well as trans-9,10-epoxystearate and the cis-8,9-, 11,12-, and 14,15- epoxyeicosatrienoic acids. The chicken enzyme displayed a lower k cat:Km for t-DPPO than the mammalian enzymes. The enzyme was sensitive to urea-based inhibitors developed for mammalian sEH. Such compounds could be used to study the role of chicken sEH in conditions in which endothelial-derived vasodilation is believed to be impaired, such as pulmonary hypertension syndrome.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 278-287 |
| Number of pages | 10 |
| Journal | Poultry Science |
| Volume | 85 |
| Issue number | 2 |
| State | Published - 2006 |
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Keywords
- Epoxyeicosatrienoic acid
- Pulmonary hypertension syndrome
- Soluble epoxide hydrolase
ASJC Scopus subject areas
- Animal Science and Zoology
Cite this
The cloning and characterization of a soluble epoxide hydrolase in chicken. / Harris, T. R.; Morisseau, C.; Walzem, R. L.; Ma, S. J.; Hammock, B. D.
In: Poultry Science, Vol. 85, No. 2, 2006, p. 278-287.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The cloning and characterization of a soluble epoxide hydrolase in chicken
AU - Harris, T. R.
AU - Morisseau, C.
AU - Walzem, R. L.
AU - Ma, S. J.
AU - Hammock, B. D.
PY - 2006
Y1 - 2006
N2 - The mammalian soluble epoxide hydrolase (sEH) plays a role in the regulation of blood pressure and vascular homeostasis through its hydrolysis of the endothelial-derived messenger molecules, the epoxyeicosatrienoic acids. This study reports the cloning and expression of a sEH homolog from chicken liver. The resulting 63-kDa protein has an isoelectric point of 6.1. The recombinant enzyme displayed epoxide hydrolase activity when assayed with [ 3H]-trans-1,3-diphenylpropene oxide (t-DPPO), as well as trans-9,10-epoxystearate and the cis-8,9-, 11,12-, and 14,15- epoxyeicosatrienoic acids. The chicken enzyme displayed a lower k cat:Km for t-DPPO than the mammalian enzymes. The enzyme was sensitive to urea-based inhibitors developed for mammalian sEH. Such compounds could be used to study the role of chicken sEH in conditions in which endothelial-derived vasodilation is believed to be impaired, such as pulmonary hypertension syndrome.
AB - The mammalian soluble epoxide hydrolase (sEH) plays a role in the regulation of blood pressure and vascular homeostasis through its hydrolysis of the endothelial-derived messenger molecules, the epoxyeicosatrienoic acids. This study reports the cloning and expression of a sEH homolog from chicken liver. The resulting 63-kDa protein has an isoelectric point of 6.1. The recombinant enzyme displayed epoxide hydrolase activity when assayed with [ 3H]-trans-1,3-diphenylpropene oxide (t-DPPO), as well as trans-9,10-epoxystearate and the cis-8,9-, 11,12-, and 14,15- epoxyeicosatrienoic acids. The chicken enzyme displayed a lower k cat:Km for t-DPPO than the mammalian enzymes. The enzyme was sensitive to urea-based inhibitors developed for mammalian sEH. Such compounds could be used to study the role of chicken sEH in conditions in which endothelial-derived vasodilation is believed to be impaired, such as pulmonary hypertension syndrome.
KW - Epoxyeicosatrienoic acid
KW - Pulmonary hypertension syndrome
KW - Soluble epoxide hydrolase
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UR - http://www.scopus.com/inward/citedby.url?scp=33645732704&partnerID=8YFLogxK
M3 - Article
C2 - 16523628
AN - SCOPUS:33645732704
VL - 85
SP - 278
EP - 287
JO - Poultry Science
JF - Poultry Science
SN - 0032-5791
IS - 2
ER -