The cloning and characterization of a soluble epoxide hydrolase in chicken

T. R. Harris, C. Morisseau, R. L. Walzem, S. J. Ma, B. D. Hammock

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The mammalian soluble epoxide hydrolase (sEH) plays a role in the regulation of blood pressure and vascular homeostasis through its hydrolysis of the endothelial-derived messenger molecules, the epoxyeicosatrienoic acids. This study reports the cloning and expression of a sEH homolog from chicken liver. The resulting 63-kDa protein has an isoelectric point of 6.1. The recombinant enzyme displayed epoxide hydrolase activity when assayed with [ 3H]-trans-1,3-diphenylpropene oxide (t-DPPO), as well as trans-9,10-epoxystearate and the cis-8,9-, 11,12-, and 14,15- epoxyeicosatrienoic acids. The chicken enzyme displayed a lower k cat:Km for t-DPPO than the mammalian enzymes. The enzyme was sensitive to urea-based inhibitors developed for mammalian sEH. Such compounds could be used to study the role of chicken sEH in conditions in which endothelial-derived vasodilation is believed to be impaired, such as pulmonary hypertension syndrome.

Original languageEnglish (US)
Pages (from-to)278-287
Number of pages10
JournalPoultry Science
Volume85
Issue number2
StatePublished - 2006

Fingerprint

epoxide hydrolase
chickens
enzymes
oxides
vasodilation
acids
isoelectric point
blood vessels
hypertension
blood pressure
homeostasis
urea
hydrolysis
lungs
cats
liver
proteins

Keywords

  • Epoxyeicosatrienoic acid
  • Pulmonary hypertension syndrome
  • Soluble epoxide hydrolase

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Harris, T. R., Morisseau, C., Walzem, R. L., Ma, S. J., & Hammock, B. D. (2006). The cloning and characterization of a soluble epoxide hydrolase in chicken. Poultry Science, 85(2), 278-287.

The cloning and characterization of a soluble epoxide hydrolase in chicken. / Harris, T. R.; Morisseau, C.; Walzem, R. L.; Ma, S. J.; Hammock, B. D.

In: Poultry Science, Vol. 85, No. 2, 2006, p. 278-287.

Research output: Contribution to journalArticle

Harris, TR, Morisseau, C, Walzem, RL, Ma, SJ & Hammock, BD 2006, 'The cloning and characterization of a soluble epoxide hydrolase in chicken', Poultry Science, vol. 85, no. 2, pp. 278-287.
Harris TR, Morisseau C, Walzem RL, Ma SJ, Hammock BD. The cloning and characterization of a soluble epoxide hydrolase in chicken. Poultry Science. 2006;85(2):278-287.
Harris, T. R. ; Morisseau, C. ; Walzem, R. L. ; Ma, S. J. ; Hammock, B. D. / The cloning and characterization of a soluble epoxide hydrolase in chicken. In: Poultry Science. 2006 ; Vol. 85, No. 2. pp. 278-287.
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