The chemistry and biology of RNA editing by adenosine deaminases.

Peter A. Beal, Olena Maydanovych, Subhash Pokharel

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Deamination of adenosines within mRNAs catalyzed by ADAR enzymes generates inosines at the corresponding nucleotide positions. Since inosine is decoded as guanosine, this reaction can lead to codon changes and the introduction of amino acids into a gene product not encoded in the gene. Translation of the different coding strands created by this process leads to protein structural diversity in the parent organism and is necessary for nervous system function in metazoa. The basis for selective editing of adenosines within certain codons is not well understood at the structural/biochemical level. Here we describe the use of synthetic nucleoside analogs incorporated into RNA editing substrates via the protected phosphoramidites to define aspects of the editing reaction mechanism and to carry out mechanism-based trapping of ADAR-RNA complexes. In addition, a high-throughput screen has been developed capable of rapidly identifying functional editing systems.

Original languageEnglish (US)
Pages (from-to)83-84
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number51
StatePublished - 2007

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RNA Editing
Inosine
Adenosine Deaminase
Codon
Adenosine
Deamination
Guanosine
Nucleosides
Nervous System
Genes
Nucleotides
RNA
Amino Acids
Messenger RNA
Enzymes
Proteins
phosphoramidite

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Beal, P. A., Maydanovych, O., & Pokharel, S. (2007). The chemistry and biology of RNA editing by adenosine deaminases. Nucleic acids symposium series (2004), (51), 83-84.

The chemistry and biology of RNA editing by adenosine deaminases. / Beal, Peter A.; Maydanovych, Olena; Pokharel, Subhash.

In: Nucleic acids symposium series (2004), No. 51, 2007, p. 83-84.

Research output: Contribution to journalArticle

Beal, PA, Maydanovych, O & Pokharel, S 2007, 'The chemistry and biology of RNA editing by adenosine deaminases.', Nucleic acids symposium series (2004), no. 51, pp. 83-84.
Beal, Peter A. ; Maydanovych, Olena ; Pokharel, Subhash. / The chemistry and biology of RNA editing by adenosine deaminases. In: Nucleic acids symposium series (2004). 2007 ; No. 51. pp. 83-84.
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