Abstract
CI-988, a water-soluble, selective cholecystokinin-B antagonist, was perfused through a microdialysis probe into the anterior nucleus accumbens, posterior nucleus accumbens, or caudate nucleus of anesthetized rats. High concentrations of CI-988 produced three- to fivefold increases in dopamine overflow, at all three sites, that were temporally correlated with the CI- 988 perfusion and returned to baseline levels upon cessation of CI-988 perfusion. However, the cholecystokinin-A antagonist CAM-1481, and the relatively inactive enantiomer of CI-988, CAM-1241, also increased dopamine overflow in the nucleus accumbens. Furthermore, the ability of CI-988 to increase dopamine overflow persisted in the absence of calcium in the perfusate and was not sensitive to tetrodotoxin treatment. The mechanism by which locally administered CI-988 increases dopamine overflow appears not to be anatomically specific, not selective for one cholecystokinin receptor subtype, and may be nonvesicular.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 208-217 |
| Number of pages | 10 |
| Journal | Journal of Neurochemistry |
| Volume | 65 |
| Issue number | 1 |
| State | Published - Jul 1995 |
| Externally published | Yes |
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Keywords
- Cholecystokinin
- In vivo microdialysis
- Mesolimbic
- Neuropeptide
- Receptor
- Release
ASJC Scopus subject areas
- Biochemistry
- Cellular and Molecular Neuroscience
Cite this
The CCK-B antagonist CI-988 increases dopamine levels in microdialysate from the rat nucleus accumbens via a tetrodotoxin- and calcium-independent mechanism. / Corwin, Rebecca L.; Jörn, Andreas; Hardy, Melva; Crawley, Jacqueline.
In: Journal of Neurochemistry, Vol. 65, No. 1, 07.1995, p. 208-217.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The CCK-B antagonist CI-988 increases dopamine levels in microdialysate from the rat nucleus accumbens via a tetrodotoxin- and calcium-independent mechanism
AU - Corwin, Rebecca L.
AU - Jörn, Andreas
AU - Hardy, Melva
AU - Crawley, Jacqueline
PY - 1995/7
Y1 - 1995/7
N2 - CI-988, a water-soluble, selective cholecystokinin-B antagonist, was perfused through a microdialysis probe into the anterior nucleus accumbens, posterior nucleus accumbens, or caudate nucleus of anesthetized rats. High concentrations of CI-988 produced three- to fivefold increases in dopamine overflow, at all three sites, that were temporally correlated with the CI- 988 perfusion and returned to baseline levels upon cessation of CI-988 perfusion. However, the cholecystokinin-A antagonist CAM-1481, and the relatively inactive enantiomer of CI-988, CAM-1241, also increased dopamine overflow in the nucleus accumbens. Furthermore, the ability of CI-988 to increase dopamine overflow persisted in the absence of calcium in the perfusate and was not sensitive to tetrodotoxin treatment. The mechanism by which locally administered CI-988 increases dopamine overflow appears not to be anatomically specific, not selective for one cholecystokinin receptor subtype, and may be nonvesicular.
AB - CI-988, a water-soluble, selective cholecystokinin-B antagonist, was perfused through a microdialysis probe into the anterior nucleus accumbens, posterior nucleus accumbens, or caudate nucleus of anesthetized rats. High concentrations of CI-988 produced three- to fivefold increases in dopamine overflow, at all three sites, that were temporally correlated with the CI- 988 perfusion and returned to baseline levels upon cessation of CI-988 perfusion. However, the cholecystokinin-A antagonist CAM-1481, and the relatively inactive enantiomer of CI-988, CAM-1241, also increased dopamine overflow in the nucleus accumbens. Furthermore, the ability of CI-988 to increase dopamine overflow persisted in the absence of calcium in the perfusate and was not sensitive to tetrodotoxin treatment. The mechanism by which locally administered CI-988 increases dopamine overflow appears not to be anatomically specific, not selective for one cholecystokinin receptor subtype, and may be nonvesicular.
KW - Cholecystokinin
KW - In vivo microdialysis
KW - Mesolimbic
KW - Neuropeptide
KW - Receptor
KW - Release
UR - http://www.scopus.com/inward/record.url?scp=0028998551&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028998551&partnerID=8YFLogxK
M3 - Article
C2 - 7790862
AN - SCOPUS:0028998551
VL - 65
SP - 208
EP - 217
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
SN - 0022-3042
IS - 1
ER -