The CALUX bio-assay: Analytical comparison between mouse hepatoma cell lines with a low (H1L6.1c3) and high (H1L7.5c1) number of dioxin response elements

Kersten Van Langenhove, Kim Croes, Michael S. Denison, Marc Elskens, Willy Baeyens

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Dioxins, furans and polychlorinated biphenyls are contaminants of high concern and as such, sensitive tools are needed to detect these persistent organic compounds in a variety of matrices. Due to the large amount of samples that need to be investigated for example for food and feed control, the CALUX bioassay (H1L6.1 clone) was developed allowing rapid and cost-efficient analysis of biological and environmental samples. Recently, a new and more sensitive clone (H1L7.5) was constructed as the third generation CALUX bioassay. This new cell line was subject of an amplification of dioxin response elements (DREs), allowing lower concentrations of target compound to be analyzed. A comparison is made between the previous, well-defined H1L6.1c3 cell line and the new H1L7.5c1 cell line: it appears that the bioassay making use of the higher number of DREs is more stable and robust, shows better repeatability and reproducibility and is; on average, 3 times more sensitive.

Original languageEnglish (US)
Pages (from-to)2039-2046
Number of pages8
JournalTalanta
Volume85
Issue number4
DOIs
StatePublished - Sep 30 2011

Keywords

  • Chemically Activated Luciferase gene expression
  • Dioxin response elements
  • EC
  • H1L6.1c3 cell line
  • H1L7.5c1 cell line
  • Polychlorinated dibenzo-p-dioxins, -furans and dioxin-like biphenyls

ASJC Scopus subject areas

  • Chemistry(all)

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