The CALUX bio-assay: Analytical comparison between mouse hepatoma cell lines with a low (H1L6.1c3) and high (H1L7.5c1) number of dioxin response elements

Kersten Van Langenhove, Kim Croes, Michael S. Denison, Marc Elskens, Willy Baeyens

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Dioxins, furans and polychlorinated biphenyls are contaminants of high concern and as such, sensitive tools are needed to detect these persistent organic compounds in a variety of matrices. Due to the large amount of samples that need to be investigated for example for food and feed control, the CALUX bioassay (H1L6.1 clone) was developed allowing rapid and cost-efficient analysis of biological and environmental samples. Recently, a new and more sensitive clone (H1L7.5) was constructed as the third generation CALUX bioassay. This new cell line was subject of an amplification of dioxin response elements (DREs), allowing lower concentrations of target compound to be analyzed. A comparison is made between the previous, well-defined H1L6.1c3 cell line and the new H1L7.5c1 cell line: it appears that the bioassay making use of the higher number of DREs is more stable and robust, shows better repeatability and reproducibility and is; on average, 3 times more sensitive.

Original languageEnglish (US)
Pages (from-to)2039-2046
Number of pages8
JournalTalanta
Volume85
Issue number4
DOIs
StatePublished - Sep 30 2011

Fingerprint

Dioxins
Bioassay
Response Elements
Biological Assay
Hepatocellular Carcinoma
Cells
Cell Line
Clone Cells
Furans
Polychlorinated Biphenyls
Organic compounds
Amplification
Impurities
Costs and Cost Analysis
Food
Costs

Keywords

  • Chemically Activated Luciferase gene expression
  • Dioxin response elements
  • EC
  • H1L6.1c3 cell line
  • H1L7.5c1 cell line
  • Polychlorinated dibenzo-p-dioxins, -furans and dioxin-like biphenyls

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

The CALUX bio-assay : Analytical comparison between mouse hepatoma cell lines with a low (H1L6.1c3) and high (H1L7.5c1) number of dioxin response elements. / Van Langenhove, Kersten; Croes, Kim; Denison, Michael S.; Elskens, Marc; Baeyens, Willy.

In: Talanta, Vol. 85, No. 4, 30.09.2011, p. 2039-2046.

Research output: Contribution to journalArticle

Van Langenhove, Kersten ; Croes, Kim ; Denison, Michael S. ; Elskens, Marc ; Baeyens, Willy. / The CALUX bio-assay : Analytical comparison between mouse hepatoma cell lines with a low (H1L6.1c3) and high (H1L7.5c1) number of dioxin response elements. In: Talanta. 2011 ; Vol. 85, No. 4. pp. 2039-2046.
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