The calcium-Ryanodine receptor complex of skeletal and cardiac muscle

Isaac N Pessah, Andrew L. Waterhouse, John E. Casida

Research output: Contribution to journalArticlepeer-review

220 Scopus citations


[3H]Ryanodine binds with high affinity to saturable and Ca2+-dependent sites in heavy sarcoplasmic reticulum (SR) preparations from rabbit skeletal and cardiac muscle. Ruthenium red, known to interfere with Ca2+-induced Ca2+ release from SR vesicles, inhibits [3H]ryanodine specific binding in both skeletal and cardiac preparations whereas Mg2+, Ba2+, Cd2+ and La3+ selectively inhibit the skeletal preparation. The toxicological relevance of the [3H]ryanodine binding site is established by the correlation of binding inhibition with toxicity for seven ryanoids including two botanical insecticides. These findings provide direct evidence for Ca2+-ryanodine receptor complexes that may play a role in excitation-contraction coupling.

Original languageEnglish (US)
Pages (from-to)449-456
Number of pages8
JournalBiochemical and Biophysical Research Communications
Issue number1
StatePublished - Apr 16 1985
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology


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