The C-681G polymorphism of the PPAR-γ gene is associated with susceptibility to non-alcoholic fatty liver disease

Chuang Yu Cao, Yu Yuan Li, Yong Jian Zhou, Yu Qiang Nie, Yu-Jui Yvonne Wan

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Non-alcoholic fatty liver disease (NAFLD) is defined as excessive accumulation of fatty acid in the liver, a common disease in the world. The research of single nucleotide polymorphisms (SNPs) provides a new approach for managing NAFLD. SNPs may increase or decrease the functions of the target genes and their encoding proteins. Peroxisome proliferator-activated receptor (PPAR) plays a key role in modulating metabolism of hepatic triglycerides and consequently magnitude of NAFLD. In this study, we investigated the effect of three SNPs in the PPAR-γ gene i.e. rs10865710 (C-681G), rs7649970 (C-689T) and rs1801282 (C34G, also termed Pro12Ala) on susceptibility to NAFLD. The participants were selected from our epidemiological survey. Totally 169 participants were enrolled in NAFLD group, and 699 healthy subjects were included as controls. PCR-RFLP was applied to detect the SNPs. The G allele frequency of rs10865710 in NAFLD group (41.1%) was significantly higher than that (34.8%) in controls (p = 0.03). Differences in other two loci (rs7649970 and rs1801282) were not statistically significant between the two groups (p > 0.05). This result was confirmed by haplotype analysis. The GCC haplotype (a set of 3 adjacent SNPs in linkage disequilibrium, corresponding to the three alleles of above polymorphisms in order) was a risk factor for the susceptibility to NAFLD (p = 0.03). This study has revealed that the G allele of rs10865710 in the PPAR-γ gene is associated with the increased susceptibility to NAFLD. Our findings may provide novel diagnostic biomarkers and therapeutic targets for NAFLD.

Original languageEnglish (US)
Pages (from-to)253-262
Number of pages10
JournalTohoku Journal of Experimental Medicine
Volume227
Issue number4
DOIs
StatePublished - 2012
Externally publishedYes

Fingerprint

Peroxisome Proliferator-Activated Receptors
Polymorphism
Liver
Genes
Single Nucleotide Polymorphism
Nucleotides
Haplotypes
Alleles
Non-alcoholic Fatty Liver Disease
Linkage Disequilibrium
Gene Frequency
Biomarkers
Restriction Fragment Length Polymorphisms
Metabolism
Healthy Volunteers
Triglycerides
Fatty Acids
Polymerase Chain Reaction

Keywords

  • Gene
  • Insulin resistance
  • Non-alcoholic fatty liver disease
  • Peroxisome proliferator-activated receptor-γ
  • Polymorphism

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

The C-681G polymorphism of the PPAR-γ gene is associated with susceptibility to non-alcoholic fatty liver disease. / Cao, Chuang Yu; Li, Yu Yuan; Zhou, Yong Jian; Nie, Yu Qiang; Wan, Yu-Jui Yvonne.

In: Tohoku Journal of Experimental Medicine, Vol. 227, No. 4, 2012, p. 253-262.

Research output: Contribution to journalArticle

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abstract = "Non-alcoholic fatty liver disease (NAFLD) is defined as excessive accumulation of fatty acid in the liver, a common disease in the world. The research of single nucleotide polymorphisms (SNPs) provides a new approach for managing NAFLD. SNPs may increase or decrease the functions of the target genes and their encoding proteins. Peroxisome proliferator-activated receptor (PPAR) plays a key role in modulating metabolism of hepatic triglycerides and consequently magnitude of NAFLD. In this study, we investigated the effect of three SNPs in the PPAR-γ gene i.e. rs10865710 (C-681G), rs7649970 (C-689T) and rs1801282 (C34G, also termed Pro12Ala) on susceptibility to NAFLD. The participants were selected from our epidemiological survey. Totally 169 participants were enrolled in NAFLD group, and 699 healthy subjects were included as controls. PCR-RFLP was applied to detect the SNPs. The G allele frequency of rs10865710 in NAFLD group (41.1{\%}) was significantly higher than that (34.8{\%}) in controls (p = 0.03). Differences in other two loci (rs7649970 and rs1801282) were not statistically significant between the two groups (p > 0.05). This result was confirmed by haplotype analysis. The GCC haplotype (a set of 3 adjacent SNPs in linkage disequilibrium, corresponding to the three alleles of above polymorphisms in order) was a risk factor for the susceptibility to NAFLD (p = 0.03). This study has revealed that the G allele of rs10865710 in the PPAR-γ gene is associated with the increased susceptibility to NAFLD. Our findings may provide novel diagnostic biomarkers and therapeutic targets for NAFLD.",
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