The bone marrow compartment is modified in the absence of galectin-3

C. Brand, F. L. Oliveira, L. Ricon, M. L. Fermino, L. C. Boldrini, D. K. Hsu, Fu-Tong Liu, R. Chammas, R. Borojevic, M. Farina, M. C. El-Cheikh

Research output: Contribution to journalArticle

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Abstract

Galectin-3 (gal-3) is a β-galactoside binding protein present in multivalent complexes with an extracellular matrix and with cell surface glycoconjugates. In this context, it can deliver a variety of intracellular signals to modulate cell activation, differentiation and survival. In the hematopoietic system, it was demonstrated that gal-3 is expressed in myeloid cells and surrounding stromal cells. Furthermore, exogenous and surface gal-3 drive the proliferation of myeloblasts in a granulocyte-macrophage colony-stimulating factor (GM-CSF)-dependent manner. Here, we investigated whether gal-3 regulates the formation of myeloid bone marrow compartments by studying galectin-3 -/- mice (gal-3 -/-) in the C57BL/6 background. The bone marrow histology of gal-3 -/- mice was significantly modified and the myeloid compartments drastically disturbed, in comparison with wild-type (WT) animals. In the absence of gal-3, we found reduced cell density and diaphyseal disorders containing increased trabecular projections into the marrow cavity. Moreover, myeloid cells presented limited capacity to differentiate into mature myeloid cell populations in gal-3 -/- mice and the number of hematopoietic multipotent progenitors was increased relative to WT animals. In addition, bone marrow stromal cells of these mice had reduced levels of GM-CSF gene expression. Taken together, our data suggest that gal-3 interferes with hematopoiesis, controlling both precursors and stromal cells and favors terminal differentiation of myeloid progenitors rather than proliferation.

Original languageEnglish (US)
Pages (from-to)427-437
Number of pages11
JournalCell and Tissue Research
Volume346
Issue number3
DOIs
StatePublished - Dec 2011

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Galectin 3
Bone Marrow
Myeloid Cells
Wild Animals
Granulocyte-Macrophage Colony-Stimulating Factor
Stromal Cells
Hematopoietic System
Galactosides
Granulocyte Precursor Cells
Glycoconjugates
Hematopoiesis
Mesenchymal Stromal Cells
Inbred C57BL Mouse
Extracellular Matrix
Cell Differentiation
Cell Survival
Histology
Carrier Proteins
Cell Count
Gene Expression

Keywords

  • Galectin-3
  • Hematopoiesis
  • Myeloid differentiation

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology
  • Histology

Cite this

Brand, C., Oliveira, F. L., Ricon, L., Fermino, M. L., Boldrini, L. C., Hsu, D. K., ... El-Cheikh, M. C. (2011). The bone marrow compartment is modified in the absence of galectin-3. Cell and Tissue Research, 346(3), 427-437. https://doi.org/10.1007/s00441-011-1276-5

The bone marrow compartment is modified in the absence of galectin-3. / Brand, C.; Oliveira, F. L.; Ricon, L.; Fermino, M. L.; Boldrini, L. C.; Hsu, D. K.; Liu, Fu-Tong; Chammas, R.; Borojevic, R.; Farina, M.; El-Cheikh, M. C.

In: Cell and Tissue Research, Vol. 346, No. 3, 12.2011, p. 427-437.

Research output: Contribution to journalArticle

Brand, C, Oliveira, FL, Ricon, L, Fermino, ML, Boldrini, LC, Hsu, DK, Liu, F-T, Chammas, R, Borojevic, R, Farina, M & El-Cheikh, MC 2011, 'The bone marrow compartment is modified in the absence of galectin-3', Cell and Tissue Research, vol. 346, no. 3, pp. 427-437. https://doi.org/10.1007/s00441-011-1276-5
Brand C, Oliveira FL, Ricon L, Fermino ML, Boldrini LC, Hsu DK et al. The bone marrow compartment is modified in the absence of galectin-3. Cell and Tissue Research. 2011 Dec;346(3):427-437. https://doi.org/10.1007/s00441-011-1276-5
Brand, C. ; Oliveira, F. L. ; Ricon, L. ; Fermino, M. L. ; Boldrini, L. C. ; Hsu, D. K. ; Liu, Fu-Tong ; Chammas, R. ; Borojevic, R. ; Farina, M. ; El-Cheikh, M. C. / The bone marrow compartment is modified in the absence of galectin-3. In: Cell and Tissue Research. 2011 ; Vol. 346, No. 3. pp. 427-437.
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