The BMP type II receptor is located in lipid rafts, including caveolae, of pulmonary endothelium in vivo and in vitro

M. Ramos, M. W. Lamé, H. J. Segall, Dennis W Wilson

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Polymorphic mutations in the Bone Morphogenetic Protein type II receptor (BMPrII) gene have been implicated in the development of familial primary pulmonary hypertension (PPH) however, the role BMPrII mutations play in the development of PH has not yet been elucidated. Endothelial caveolae are an important domain of hemodynamics containing eNOS, the serotonin transporter, and endothelin receptors. In this study we show by standard immunohistochemistry (IHC) that BMPrII is widely distributed in the vasculature of the rat lung, and more specifically distributed to both apical and basal membranes of the arteriolar endothelium by fluorescent IHC. We also examined compartmentalization of BMPrII in lipid fractions of plasma membranes isolated by silica based extraction from human pulmonary artery endothelial cells and rat lung endothelium. Density gradient centrifugation demonstrated BMPrII in separate caveolin-1 (cav-1) and non-cav-1 lipid rich fractions. Electron microscopy co-localized cav-1 and BMPrII in flask shaped membrane fragments. Three-dimensional fluorescence microscopy demonstrated BMPrII in discrete membrane foci, a portion of which were co-localized with cav-1, as well as in Golgi. Our findings indicate that BMPrII is located within lipid-dense fractions of pulmonary endothelial cell membranes with a portion present in caveolae suggesting potential dynamic regulatory structural relationships.

Original languageEnglish (US)
Pages (from-to)50-59
Number of pages10
JournalVascular Pharmacology
Volume44
Issue number1
DOIs
StatePublished - Jan 2006

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Type II Bone Morphogenetic Protein Receptors
Caveolae
Endothelium
Lipids
Lung
Caveolin 1
Membranes
Endothelial Cells
Immunohistochemistry
Cell Membrane
Endothelin Receptors
Serotonin Plasma Membrane Transport Proteins
Mutation
Density Gradient Centrifugation
In Vitro Techniques
Serotonin Receptors
Fluorescence Microscopy
Silicon Dioxide
Pulmonary Artery
Electron Microscopy

Keywords

  • Bone morphogenetic protein type II receptor
  • Caveolae
  • Pulmonary hypertension

ASJC Scopus subject areas

  • Pharmacology

Cite this

The BMP type II receptor is located in lipid rafts, including caveolae, of pulmonary endothelium in vivo and in vitro. / Ramos, M.; Lamé, M. W.; Segall, H. J.; Wilson, Dennis W.

In: Vascular Pharmacology, Vol. 44, No. 1, 01.2006, p. 50-59.

Research output: Contribution to journalArticle

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