The bipolar assembly domain of the mitotic motor kinesin-5

Seyda Acar; David B. Carlson; Madhu S. Budamagunta; Vladimir Yarov-Yarovoy; John J. Correia; Milady R. Niñonuevo; Weitao Jia; Li Tao; Julie A. Leary; John C Voss; James E. Evans; Jonathan M. Scholey

doi:10.1038/ncomms2348

The bipolar assembly domain of the mitotic motor kinesin-5

Seyda Acar, David B. Carlson, Madhu S. Budamagunta, Vladimir Yarov-Yarovoy, John J. Correia, Milady R. Niñonuevo, Weitao Jia, Li Tao, Julie A. Leary, John C Voss, James E. Evans, Jonathan M. Scholey

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

An outstanding unresolved question is how does the mitotic spindle utilize microtubules and mitotic motors to coordinate accurate chromosome segregation during mitosis? This process depends upon the mitotic motor, kinesin-5, whose unique bipolar architecture, with pairs of motor domains lying at opposite ends of a central rod, allows it to crosslink microtubules within the mitotic spindle and to coordinate their relative sliding during spindle assembly, maintenance and elongation. The structural basis of kinesin-5's bipolarity is, however, unknown, as protein asymmetry has so far precluded its crystallization. Here we use electron microscopy of single molecules of kinesin-5 and its subfragments, combined with hydrodynamic analysis plus mass spectrometry, circular dichroism and site-directed spin label electron paramagnetic resonance spectroscopy, to show how a staggered antiparallel coiled-coil 'BASS' (bipolar assembly) domain directs the assembly of four kinesin-5 polypeptides into bipolar minifilaments.

Original language	English (US)
Article number	1343
Journal	Nature Communications
Volume	4
DOIs	https://doi.org/10.1038/ncomms2348
State	Published - 2013

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Chemistry(all)
Physics and Astronomy(all)

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The bipolar assembly domain of the mitotic motor kinesin-5. / Acar, Seyda; Carlson, David B.; Budamagunta, Madhu S.; Yarov-Yarovoy, Vladimir; Correia, John J.; Niñonuevo, Milady R.; Jia, Weitao; Tao, Li; Leary, Julie A.; Voss, John C; Evans, James E.; Scholey, Jonathan M.

In: Nature Communications, Vol. 4, 1343, 2013.

Research output: Contribution to journal › Article › peer-review

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title = "The bipolar assembly domain of the mitotic motor kinesin-5",

abstract = "An outstanding unresolved question is how does the mitotic spindle utilize microtubules and mitotic motors to coordinate accurate chromosome segregation during mitosis? This process depends upon the mitotic motor, kinesin-5, whose unique bipolar architecture, with pairs of motor domains lying at opposite ends of a central rod, allows it to crosslink microtubules within the mitotic spindle and to coordinate their relative sliding during spindle assembly, maintenance and elongation. The structural basis of kinesin-5's bipolarity is, however, unknown, as protein asymmetry has so far precluded its crystallization. Here we use electron microscopy of single molecules of kinesin-5 and its subfragments, combined with hydrodynamic analysis plus mass spectrometry, circular dichroism and site-directed spin label electron paramagnetic resonance spectroscopy, to show how a staggered antiparallel coiled-coil 'BASS' (bipolar assembly) domain directs the assembly of four kinesin-5 polypeptides into bipolar minifilaments.",

author = "Seyda Acar and Carlson, {David B.} and Budamagunta, {Madhu S.} and Vladimir Yarov-Yarovoy and Correia, {John J.} and Ni{\~n}onuevo, {Milady R.} and Weitao Jia and Li Tao and Leary, {Julie A.} and Voss, {John C} and Evans, {James E.} and Scholey, {Jonathan M.}",

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AU - Budamagunta, Madhu S.

AU - Yarov-Yarovoy, Vladimir

AU - Correia, John J.

AU - Niñonuevo, Milady R.

AU - Jia, Weitao

AU - Tao, Li

AU - Leary, Julie A.

AU - Voss, John C

AU - Evans, James E.

AU - Scholey, Jonathan M.

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