The binding selectivity of ADAR2's dsRBMs contributes to RNA-editing selectivity

Olen M. Stephens, Brittany L. Haudenschild, Peter A. Beal

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

ADAR2 is an RNA editing enzyme that deaminates adenosines in certain duplex structures. Here, we describe the role of its RNA binding domain, consisting of two copies of a common dsRNA binding motif (dsRBM), in editing site selecivity. ADAR2's dsRBMs bind selectively on a duplex RNA that mimics the Q/R editing site in the glutamate receptor B-subunit pre-mRNA. This selectivity is different from that of PKR's dsRBM I, indicating that dsRBMs from different proteins possess intrinsic binding selectivity. Using directed hydroxyl radical cleavage data, molecular models were developed that predict important recognition surfaces on the RNA for identified dsRBM binding sites. Blocking these surfaces by benzyl modification of guanosine 2-amino groups impeded RNA-editing, demonstrating a correlation between deamination efficiency by ADAR2 and selective binding by its dsRBMs. In addition, the editing activity of a mutant of ADAR2 lacking dsRBM I on N2-benzylguanosine-modified RNA suggests the location of the dsRBM I binding site that leads to editing at the GluR-B Q/R site.

Original languageEnglish (US)
Pages (from-to)1239-1250
Number of pages12
JournalChemistry and Biology
Volume11
Issue number9
DOIs
StatePublished - Sep 2004
Externally publishedYes

ASJC Scopus subject areas

  • Organic Chemistry

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