The attachment of metal-chelating groups to proteins: Tagging of albumin by diazonium coupling and use of the products as radiopharmaceuticals

C. S H Leung, C. F. Meares, D. A. Goodwin

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

The ability to attach firmly chelated metal ions or powerful chelating agents to sites on biological molecules can enhance the utility of a number of physical techniques now used in the study of biological systems. A "bifunctional" chelating agent, containing both an EDTA group and a diazonium group, has been prepared and coupled to human serum albumin. The extent of labeling under various conditions and the amino-acid sidechains labeled have been investigated. The reaction of protein-bound chelating groups with added metal ions has been studied, with the finding that only about 40-50% of these groups are available to bind metal ions. Proteolysis of the products leads to recovery of full metal-binding capacity. Properties of the products in vivo are discussed.

Original languageEnglish (US)
Pages (from-to)687-692
Number of pages6
JournalThe International Journal Of Applied Radiation And Isotopes
Volume29
Issue number11
DOIs
StatePublished - 1978

ASJC Scopus subject areas

  • Nuclear Energy and Engineering
  • Radiology Nuclear Medicine and imaging
  • Radiation

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