The ability to attach firmly chelated metal ions or powerful chelating agents to sites on biological molecules can enhance the utility of a number of physical techniques now used in the study of biological systems. A "bifunctional" chelating agent, containing both an EDTA group and a diazonium group, has been prepared and coupled to human serum albumin. The extent of labeling under various conditions and the amino-acid sidechains labeled have been investigated. The reaction of protein-bound chelating groups with added metal ions has been studied, with the finding that only about 40-50% of these groups are available to bind metal ions. Proteolysis of the products leads to recovery of full metal-binding capacity. Properties of the products in vivo are discussed.
|Original language||English (US)|
|Number of pages||6|
|Journal||The International Journal Of Applied Radiation And Isotopes|
|State||Published - 1978|
ASJC Scopus subject areas
- Nuclear Energy and Engineering
- Radiology Nuclear Medicine and imaging