The antinuclear autoantibodies Sp100 and gp210 persist after orthotopic liver transplantation in patients with primary biliary cirrhosis

Birgit Luettig, Klaus H W Boeker, Werner Schoessler, Hans Will, Stephanie Loges, Eleonore Schmidt, Howard J. Worman, M. Eric Gershwin, Michael P. Manns

Research output: Contribution to journalArticle

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Abstract

Background/Aims: Primary biliary cirrhosis is an autoimmune liver disease which is characterized by the presence of autoantibodies directed against mitochondrial components which belong to the pyruvate dehydrogenase enzyme complex. Apart from antibodies against mitochondrial components, primary biliary cirrhosis patients often show antibodies against nuclear components, of which anti-Sp100 and anti-gp210 are considered to be disease specific. We investigated the incidence and course of antibodies against nuclear components in primary biliary cirrhosis patients before and after liver transplantation. Methods: Sera from 42 primary biliary cirrhosis patients were studied using indirect immunofluorescence to detect antibodies against mitochondrial components and antibodies against nuclear components, ELISA to detect anti-Sp100, and immunoblot analysis to detect anti-gp210 and antibodies against nuclear components subtypes. Results: Ninety-three percent of primary biliary cirrhosis patients in our study were antimitochondrial antibody positive. Forty-three percent of the patients were antinuclear antibody positive. Of these, 35% had antibodies against Sp100 and 36% were positive for anti-gp210. After transplantation, antimitochondrial antibody titers as well as antinuclear antibody titers decreased in all patients. Autoantibodies in low titer persisted for up to 13 years. The pattern of nuclear autoantigens recognized by patient sera was unchanged after fiver transplantation. However, the antinuclear antibody pattern was very different between the individual patients. Anti-Sp100 and anti-gp210 were not detected in sera of patients with autoimmune hepatitis, hepatitis C infection, inflammatory bowel disease, connective tissue diseases, or primary sclerosing cholangitis. The serum alkaline phosphatase level was not different in antinuclear antibody negative or positive patients before or after transplantation. Conclusions: We conclude that the persistence of antibodies against mitochondrial components, and antiSp100 and anti-gp210 in primary biliary Cirrhosis patients after liver transplantation is disease specific, but that this does not reflect recurrent disease activity in the graft.

Original languageEnglish (US)
Pages (from-to)824-828
Number of pages5
JournalJournal of Hepatology
Volume28
Issue number5
DOIs
StatePublished - May 1998

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Biliary Liver Cirrhosis
Liver Transplantation
Autoantibodies
Antibodies
Antinuclear Antibodies
Transplantation
Serum
Pyruvate Dehydrogenase Complex
Autoimmune Hepatitis
Sclerosing Cholangitis
Connective Tissue Diseases
Autoantigens
Indirect Fluorescent Antibody Technique
Hepatitis C
Inflammatory Bowel Diseases
Autoimmune Diseases
Alkaline Phosphatase
Liver Diseases
Anti-Idiotypic Antibodies
Enzyme-Linked Immunosorbent Assay

Keywords

  • Antinuclear autoantibodies
  • Autoantigen
  • Autoimmunity
  • Cholestasis
  • Gp210
  • Orthotopic liver transplantation
  • Primary biliary cirrhosis
  • Sp100

ASJC Scopus subject areas

  • Gastroenterology

Cite this

The antinuclear autoantibodies Sp100 and gp210 persist after orthotopic liver transplantation in patients with primary biliary cirrhosis. / Luettig, Birgit; Boeker, Klaus H W; Schoessler, Werner; Will, Hans; Loges, Stephanie; Schmidt, Eleonore; Worman, Howard J.; Gershwin, M. Eric; Manns, Michael P.

In: Journal of Hepatology, Vol. 28, No. 5, 05.1998, p. 824-828.

Research output: Contribution to journalArticle

Luettig, Birgit ; Boeker, Klaus H W ; Schoessler, Werner ; Will, Hans ; Loges, Stephanie ; Schmidt, Eleonore ; Worman, Howard J. ; Gershwin, M. Eric ; Manns, Michael P. / The antinuclear autoantibodies Sp100 and gp210 persist after orthotopic liver transplantation in patients with primary biliary cirrhosis. In: Journal of Hepatology. 1998 ; Vol. 28, No. 5. pp. 824-828.
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abstract = "Background/Aims: Primary biliary cirrhosis is an autoimmune liver disease which is characterized by the presence of autoantibodies directed against mitochondrial components which belong to the pyruvate dehydrogenase enzyme complex. Apart from antibodies against mitochondrial components, primary biliary cirrhosis patients often show antibodies against nuclear components, of which anti-Sp100 and anti-gp210 are considered to be disease specific. We investigated the incidence and course of antibodies against nuclear components in primary biliary cirrhosis patients before and after liver transplantation. Methods: Sera from 42 primary biliary cirrhosis patients were studied using indirect immunofluorescence to detect antibodies against mitochondrial components and antibodies against nuclear components, ELISA to detect anti-Sp100, and immunoblot analysis to detect anti-gp210 and antibodies against nuclear components subtypes. Results: Ninety-three percent of primary biliary cirrhosis patients in our study were antimitochondrial antibody positive. Forty-three percent of the patients were antinuclear antibody positive. Of these, 35{\%} had antibodies against Sp100 and 36{\%} were positive for anti-gp210. After transplantation, antimitochondrial antibody titers as well as antinuclear antibody titers decreased in all patients. Autoantibodies in low titer persisted for up to 13 years. The pattern of nuclear autoantigens recognized by patient sera was unchanged after fiver transplantation. However, the antinuclear antibody pattern was very different between the individual patients. Anti-Sp100 and anti-gp210 were not detected in sera of patients with autoimmune hepatitis, hepatitis C infection, inflammatory bowel disease, connective tissue diseases, or primary sclerosing cholangitis. The serum alkaline phosphatase level was not different in antinuclear antibody negative or positive patients before or after transplantation. Conclusions: We conclude that the persistence of antibodies against mitochondrial components, and antiSp100 and anti-gp210 in primary biliary Cirrhosis patients after liver transplantation is disease specific, but that this does not reflect recurrent disease activity in the graft.",
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T1 - The antinuclear autoantibodies Sp100 and gp210 persist after orthotopic liver transplantation in patients with primary biliary cirrhosis

AU - Luettig, Birgit

AU - Boeker, Klaus H W

AU - Schoessler, Werner

AU - Will, Hans

AU - Loges, Stephanie

AU - Schmidt, Eleonore

AU - Worman, Howard J.

AU - Gershwin, M. Eric

AU - Manns, Michael P.

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AB - Background/Aims: Primary biliary cirrhosis is an autoimmune liver disease which is characterized by the presence of autoantibodies directed against mitochondrial components which belong to the pyruvate dehydrogenase enzyme complex. Apart from antibodies against mitochondrial components, primary biliary cirrhosis patients often show antibodies against nuclear components, of which anti-Sp100 and anti-gp210 are considered to be disease specific. We investigated the incidence and course of antibodies against nuclear components in primary biliary cirrhosis patients before and after liver transplantation. Methods: Sera from 42 primary biliary cirrhosis patients were studied using indirect immunofluorescence to detect antibodies against mitochondrial components and antibodies against nuclear components, ELISA to detect anti-Sp100, and immunoblot analysis to detect anti-gp210 and antibodies against nuclear components subtypes. Results: Ninety-three percent of primary biliary cirrhosis patients in our study were antimitochondrial antibody positive. Forty-three percent of the patients were antinuclear antibody positive. Of these, 35% had antibodies against Sp100 and 36% were positive for anti-gp210. After transplantation, antimitochondrial antibody titers as well as antinuclear antibody titers decreased in all patients. Autoantibodies in low titer persisted for up to 13 years. The pattern of nuclear autoantigens recognized by patient sera was unchanged after fiver transplantation. However, the antinuclear antibody pattern was very different between the individual patients. Anti-Sp100 and anti-gp210 were not detected in sera of patients with autoimmune hepatitis, hepatitis C infection, inflammatory bowel disease, connective tissue diseases, or primary sclerosing cholangitis. The serum alkaline phosphatase level was not different in antinuclear antibody negative or positive patients before or after transplantation. Conclusions: We conclude that the persistence of antibodies against mitochondrial components, and antiSp100 and anti-gp210 in primary biliary Cirrhosis patients after liver transplantation is disease specific, but that this does not reflect recurrent disease activity in the graft.

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KW - Cholestasis

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