The Antibody Society’s antibody validation webinar series

Jan L.A. Voskuil, Anita Bandrowski, C. Glenn Begley, Andrew R.M. Bradbury, Andrew D. Chalmers, Aldrin V. Gomes, Travis Hardcastle, Fridtjof Lund-Johansen, Andreas Plückthun, Giovanna Roncador, Alejandra Solache, Michael J. Taussig, James Trimmer, Cecilia Williams, Simon L. Goodman

Research output: Contribution to journalArticle

Abstract

In the wake of the reproducibility crisis and numerous discussions on how commercially available antibodies as research tool contribute to it, The Antibody Society developed a series of 10 webinars to address the issues involved. The webinars were delivered by speakers with both academic and commercial backgrounds. This report highlights the problems, and offers solutions to help the scientific community appropriately identify the right antibodies and to validate them for their research and development projects. Despite the various solutions proposed here, they must be applied on a case-by-case basis. Each antibody must be verified based on the content of the product sheet, and subsequently through experimentation to confirm integrity, specificity and selectivity. Verification needs to focus on the precise application and tissue/cell type for which the antibody will be used, and all verification data must be reported openly. The various approaches discussed here all have caveats, so a combination of solutions must be considered.

Original languageEnglish (US)
Article number1794421
JournalmAbs
Volume12
Issue number1
DOIs
StatePublished - Jan 1 2020

Keywords

  • antibody selectivity
  • Antibody specificity
  • antibody validation
  • monoclonal antibodies
  • peptide antibodies
  • polyclonal antibodies
  • recombinant antibodies
  • reproducibility
  • selective antibodies
  • specific antibodies

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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  • Cite this

    Voskuil, J. L. A., Bandrowski, A., Begley, C. G., Bradbury, A. R. M., Chalmers, A. D., Gomes, A. V., Hardcastle, T., Lund-Johansen, F., Plückthun, A., Roncador, G., Solache, A., Taussig, M. J., Trimmer, J., Williams, C., & Goodman, S. L. (2020). The Antibody Society’s antibody validation webinar series. mAbs, 12(1), [1794421]. https://doi.org/10.1080/19420862.2020.1794421