The anti-inflammatory effects of soluble epoxide hydrolase inhibitors are independent of leukocyte recruitment

Benjamin B. Davis, Jun Yan Liu, Daniel J Tancredi, Lei Wang, Scott I. Simon, Bruce D. Hammock, Kent E Pinkerton

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Excess leukocyte recruitment to the lung plays a central role in the development or exacerbation of several lung inflammatory diseases including chronic obstructive pulmonary disease. Epoxyeicosatrienoic acids (EETs) are cytochrome P-450 metabolites of arachidonic acid reported to have multiple biological functions, including blocking of leukocyte recruitment to inflamed endothelium in cell culture through reduction of adhesion molecule expression. Inhibition of the EET regulatory enzyme, soluble epoxide hydrolase (sEH) also has been reported to have anti-inflammatory effects in vivo including reduced leukocyte recruitment to the lung. We tested the hypothesis that the in vivo anti-inflammatory effects of sEH inhibitors act through the same mechanisms as the in vitro anti-inflammatory effects of EETs in a rat model of acute inflammation following exposure to tobacco smoke. Contrary to previously published data, we found that sEH inhibition did not reduce tobacco smoke-induced leukocyte recruitment to the lung. Furthermore, sEH inhibition did not reduce tobacco smoke-induced adhesion molecule expression in the lung vasculature. Similarly, concentrations of EETs greater than or equal to their reported effective dose did not reduce TNFα induced expression of the adhesion molecules. These results suggest that the anti-inflammatory effects of sEH inhibitors are independent of leukocyte recruitment and EETs do not reduce the adhesion molecules responsible for leukocyte recruitment in vitro. This demonstrates that the widely held belief that sEH inhibition prevents leukocyte recruitment via EET prevention of adhesion molecule expression is not consistently reproducible.

Original languageEnglish (US)
Pages (from-to)494-500
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume410
Issue number3
DOIs
StatePublished - Jul 8 2011

Fingerprint

Epoxide Hydrolases
Leukocytes
Anti-Inflammatory Agents
Tobacco
Adhesion
Smoke
Lung
Pulmonary diseases
Molecules
Cell Adhesion Molecules
Metabolites
Cell culture
Arachidonic Acid
Cytochrome P-450 Enzyme System
Chronic Obstructive Pulmonary Disease
Lung Diseases
Endothelium
Rats
Cell Culture Techniques
Inflammation

Keywords

  • Adhesion molecule
  • Chronic obstructive pulmonary disease
  • Epoxyeicosatrienoic acids
  • Inflammation
  • Leukocyte recruitment
  • Lipids
  • Lung
  • Soluble epoxide hydrolase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

The anti-inflammatory effects of soluble epoxide hydrolase inhibitors are independent of leukocyte recruitment. / Davis, Benjamin B.; Liu, Jun Yan; Tancredi, Daniel J; Wang, Lei; Simon, Scott I.; Hammock, Bruce D.; Pinkerton, Kent E.

In: Biochemical and Biophysical Research Communications, Vol. 410, No. 3, 08.07.2011, p. 494-500.

Research output: Contribution to journalArticle

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