The Amino-Terminal Region of Major Capsid Protein P3 Is Essential for Self-Assembly of Single-Shelled Core-Like Particles of Rice Dwarf Virus

Kyoji Hagiwara, Takahiko Higashi, Naoyuki Miyazaki, Hisashi Naitow, R. Holland Cheng, Atsushi Nakagawa, Hiroshi Mizuno, Tomitake Tsukihara, Toshihiro Omura

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

The core protein P3 of Rice dwarf virus constructs asymmetric dimers, one of which is inserted by the amino-terminal region of another P3 protein. The P3 proteins with serial amino-terminal deletions, expressed in a baculovirus system, formed particles with gradually decreasing stability. The capacity for self-assembly disappeared when 52 of the amino-terminal amino acids had been deleted. These results demonstrated that insertion of the amino-terminal arm of one P3 protein into another appears to play an important role in stabilizing the core particles.

Original languageEnglish (US)
Pages (from-to)3145-3148
Number of pages4
JournalJournal of Virology
Volume78
Issue number6
DOIs
StatePublished - Mar 2004
Externally publishedYes

ASJC Scopus subject areas

  • Immunology

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