The AMIGO1 adhesion protein activates Kv2.1 voltage sensors

Rebecka J. Sepela, Robert G. Stewart, Luis A. Valencia, Parashar Thapa, Zeming Wang, Bruce E. Cohen, Jon T. Sack

Research output: Contribution to journalArticlepeer-review


Kv2 voltage-gated potassium channels are modulated by amphoterin-induced gene and open reading frame (AMIGO) neuronal adhesion proteins. Here, we identify steps in the conductance activation pathway of Kv2.1 channels that are modulated by AMIGO1 using voltage-clamp recordings and spectroscopy of heterologously expressed Kv2.1 and AMIGO1 in mammalian cell lines. AMIGO1 speeds early voltage-sensor movements and shifts the gating charge-voltage relationship to more negative voltages. The gating charge-voltage relationship indicates that AMIGO1 exerts a larger energetic effect on voltage-sensor movement than is apparent from the midpoint of the conductance-voltage relationship. When voltage sensors are detained at rest by voltage-sensor toxins, AMIGO1 has a greater impact on the conductance-voltage relationship. Fluorescence measurements from voltage-sensor toxins bound to Kv2.1 indicate that with AMIGO1, the voltage sensors enter their earliest resting conformation, yet this conformation is less stable upon voltage stimulation. We conclude that AMIGO1 modulates the Kv2.1 conductance activation pathway by destabilizing the earliest resting state of the voltage sensors.

Original languageEnglish (US)
Pages (from-to)1395-1416
Number of pages22
JournalBiophysical Journal
Issue number8
StatePublished - Apr 19 2022

ASJC Scopus subject areas

  • Biophysics


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