The alpha linolenic acid content of flaxseed is associated with an induction of adipose leptin expression

Richelle S. Mc Cullough, Andrea L. Edel, Chantal M.C. Bassett, Renée K. Lavallée, Elena Dibrov, David P. Blackwood, Bradley P. Ander, Grant N. Pierce

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Dietary flaxseed has cardioprotective effects that may be achieved through its rich content of the omega-3 fatty acid, alpha linolenic acid (ALA). Because ALA can be stored in adipose tissue, it is possible that some of its beneficial actions may be due to effects it has on the adipose tissue. We investigated the effects of dietary flaxseed both with and without an atherogenic cholesterol-enriched diet to determine the effects of dietary flaxseed on the expression of the adipose cytokines leptin and adiponectin. Rabbits were fed one of four diets: a regular (RG) diet, or a regular diet with added 0.5% cholesterol (CH), or 10% ground flaxseed (FX), or both (CF) for 8 weeks. Levels of leptin and adiponectin expression were assessed by RT-PCR in visceral adipose tissue. Consumption of flaxseed significantly increased plasma and adipose levels of ALA. Leptin protein and mRNA expression were lower in CH animals and were elevated in CF animals. Changes in leptin expression were strongly and positively correlated with adipose ALA levels and inversely correlated with levels of en face atherosclerosis. Adiponectin expression was not significantly affected by any of the dietary interventions. Our data demonstrate that the type of fat in the diet as well as its caloric content can specifically influence leptin expression. The findings support the hypothesis that the beneficial cardiovascular effects associated with flaxseed consumption may be related to a change in leptin expression.

Original languageEnglish (US)
Pages (from-to)1043-1052
Number of pages10
JournalLipids
Volume46
Issue number11
DOIs
StatePublished - Nov 1 2011
Externally publishedYes

Fingerprint

Flax
alpha-Linolenic Acid
Leptin
Nutrition
Diet
Adiponectin
Cholesterol
Tissue
Adipose Tissue
Animals
Intra-Abdominal Fat
Omega-3 Fatty Acids
Atherosclerosis
Fats
Cytokines
Rabbits
Plasmas
Polymerase Chain Reaction
Messenger RNA
Proteins

Keywords

  • Adipokine
  • Adiponectin
  • Adipose tissue
  • Alpha linolenic acid
  • Atherosclerosis
  • Cholesterol
  • Flaxseed
  • Leptin

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Cell Biology

Cite this

Mc Cullough, R. S., Edel, A. L., Bassett, C. M. C., Lavallée, R. K., Dibrov, E., Blackwood, D. P., ... Pierce, G. N. (2011). The alpha linolenic acid content of flaxseed is associated with an induction of adipose leptin expression. Lipids, 46(11), 1043-1052. https://doi.org/10.1007/s11745-011-3619-0

The alpha linolenic acid content of flaxseed is associated with an induction of adipose leptin expression. / Mc Cullough, Richelle S.; Edel, Andrea L.; Bassett, Chantal M.C.; Lavallée, Renée K.; Dibrov, Elena; Blackwood, David P.; Ander, Bradley P.; Pierce, Grant N.

In: Lipids, Vol. 46, No. 11, 01.11.2011, p. 1043-1052.

Research output: Contribution to journalArticle

Mc Cullough, RS, Edel, AL, Bassett, CMC, Lavallée, RK, Dibrov, E, Blackwood, DP, Ander, BP & Pierce, GN 2011, 'The alpha linolenic acid content of flaxseed is associated with an induction of adipose leptin expression', Lipids, vol. 46, no. 11, pp. 1043-1052. https://doi.org/10.1007/s11745-011-3619-0
Mc Cullough RS, Edel AL, Bassett CMC, Lavallée RK, Dibrov E, Blackwood DP et al. The alpha linolenic acid content of flaxseed is associated with an induction of adipose leptin expression. Lipids. 2011 Nov 1;46(11):1043-1052. https://doi.org/10.1007/s11745-011-3619-0
Mc Cullough, Richelle S. ; Edel, Andrea L. ; Bassett, Chantal M.C. ; Lavallée, Renée K. ; Dibrov, Elena ; Blackwood, David P. ; Ander, Bradley P. ; Pierce, Grant N. / The alpha linolenic acid content of flaxseed is associated with an induction of adipose leptin expression. In: Lipids. 2011 ; Vol. 46, No. 11. pp. 1043-1052.
@article{dbd9c46bf2fb4cd8b57ab7dacf2cf05c,
title = "The alpha linolenic acid content of flaxseed is associated with an induction of adipose leptin expression",
abstract = "Dietary flaxseed has cardioprotective effects that may be achieved through its rich content of the omega-3 fatty acid, alpha linolenic acid (ALA). Because ALA can be stored in adipose tissue, it is possible that some of its beneficial actions may be due to effects it has on the adipose tissue. We investigated the effects of dietary flaxseed both with and without an atherogenic cholesterol-enriched diet to determine the effects of dietary flaxseed on the expression of the adipose cytokines leptin and adiponectin. Rabbits were fed one of four diets: a regular (RG) diet, or a regular diet with added 0.5{\%} cholesterol (CH), or 10{\%} ground flaxseed (FX), or both (CF) for 8 weeks. Levels of leptin and adiponectin expression were assessed by RT-PCR in visceral adipose tissue. Consumption of flaxseed significantly increased plasma and adipose levels of ALA. Leptin protein and mRNA expression were lower in CH animals and were elevated in CF animals. Changes in leptin expression were strongly and positively correlated with adipose ALA levels and inversely correlated with levels of en face atherosclerosis. Adiponectin expression was not significantly affected by any of the dietary interventions. Our data demonstrate that the type of fat in the diet as well as its caloric content can specifically influence leptin expression. The findings support the hypothesis that the beneficial cardiovascular effects associated with flaxseed consumption may be related to a change in leptin expression.",
keywords = "Adipokine, Adiponectin, Adipose tissue, Alpha linolenic acid, Atherosclerosis, Cholesterol, Flaxseed, Leptin",
author = "{Mc Cullough}, {Richelle S.} and Edel, {Andrea L.} and Bassett, {Chantal M.C.} and Lavall{\'e}e, {Ren{\'e}e K.} and Elena Dibrov and Blackwood, {David P.} and Ander, {Bradley P.} and Pierce, {Grant N.}",
year = "2011",
month = "11",
day = "1",
doi = "10.1007/s11745-011-3619-0",
language = "English (US)",
volume = "46",
pages = "1043--1052",
journal = "Lipids",
issn = "0024-4201",
publisher = "Springer Verlag",
number = "11",

}

TY - JOUR

T1 - The alpha linolenic acid content of flaxseed is associated with an induction of adipose leptin expression

AU - Mc Cullough, Richelle S.

AU - Edel, Andrea L.

AU - Bassett, Chantal M.C.

AU - Lavallée, Renée K.

AU - Dibrov, Elena

AU - Blackwood, David P.

AU - Ander, Bradley P.

AU - Pierce, Grant N.

PY - 2011/11/1

Y1 - 2011/11/1

N2 - Dietary flaxseed has cardioprotective effects that may be achieved through its rich content of the omega-3 fatty acid, alpha linolenic acid (ALA). Because ALA can be stored in adipose tissue, it is possible that some of its beneficial actions may be due to effects it has on the adipose tissue. We investigated the effects of dietary flaxseed both with and without an atherogenic cholesterol-enriched diet to determine the effects of dietary flaxseed on the expression of the adipose cytokines leptin and adiponectin. Rabbits were fed one of four diets: a regular (RG) diet, or a regular diet with added 0.5% cholesterol (CH), or 10% ground flaxseed (FX), or both (CF) for 8 weeks. Levels of leptin and adiponectin expression were assessed by RT-PCR in visceral adipose tissue. Consumption of flaxseed significantly increased plasma and adipose levels of ALA. Leptin protein and mRNA expression were lower in CH animals and were elevated in CF animals. Changes in leptin expression were strongly and positively correlated with adipose ALA levels and inversely correlated with levels of en face atherosclerosis. Adiponectin expression was not significantly affected by any of the dietary interventions. Our data demonstrate that the type of fat in the diet as well as its caloric content can specifically influence leptin expression. The findings support the hypothesis that the beneficial cardiovascular effects associated with flaxseed consumption may be related to a change in leptin expression.

AB - Dietary flaxseed has cardioprotective effects that may be achieved through its rich content of the omega-3 fatty acid, alpha linolenic acid (ALA). Because ALA can be stored in adipose tissue, it is possible that some of its beneficial actions may be due to effects it has on the adipose tissue. We investigated the effects of dietary flaxseed both with and without an atherogenic cholesterol-enriched diet to determine the effects of dietary flaxseed on the expression of the adipose cytokines leptin and adiponectin. Rabbits were fed one of four diets: a regular (RG) diet, or a regular diet with added 0.5% cholesterol (CH), or 10% ground flaxseed (FX), or both (CF) for 8 weeks. Levels of leptin and adiponectin expression were assessed by RT-PCR in visceral adipose tissue. Consumption of flaxseed significantly increased plasma and adipose levels of ALA. Leptin protein and mRNA expression were lower in CH animals and were elevated in CF animals. Changes in leptin expression were strongly and positively correlated with adipose ALA levels and inversely correlated with levels of en face atherosclerosis. Adiponectin expression was not significantly affected by any of the dietary interventions. Our data demonstrate that the type of fat in the diet as well as its caloric content can specifically influence leptin expression. The findings support the hypothesis that the beneficial cardiovascular effects associated with flaxseed consumption may be related to a change in leptin expression.

KW - Adipokine

KW - Adiponectin

KW - Adipose tissue

KW - Alpha linolenic acid

KW - Atherosclerosis

KW - Cholesterol

KW - Flaxseed

KW - Leptin

UR - http://www.scopus.com/inward/record.url?scp=83555174339&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=83555174339&partnerID=8YFLogxK

U2 - 10.1007/s11745-011-3619-0

DO - 10.1007/s11745-011-3619-0

M3 - Article

C2 - 22031167

AN - SCOPUS:83555174339

VL - 46

SP - 1043

EP - 1052

JO - Lipids

JF - Lipids

SN - 0024-4201

IS - 11

ER -