The advanced glycation endproduct pentosidine induces the expression of PDGF-B in human retinal pigment epithelial cells

James T. Handa, Karen M. Reiser, Hiroshi Matsunaga, Leonard M Hjelmeland

Research output: Contribution to journalArticle

54 Scopus citations

Abstract

Advanced glycation endproducts have been implicated in a number of diabetic and aging changes. Some of these effects occur in part through induction of cytokines such as platelet-derived growth factor (PDGF), which is expressed by the retinal pigment epithelium (RPE). In this study, cultures of RPE were evaluated for PDGF expression after treatment with pentosidine, a well characterized advanced glycation endproduct. Northern analysis provided evidence for the increased expression of a 3.7 kb PDGF-B transcript over unstimulated controls in the established ARPE-19 cell line. Western analysis demonstrated increased PDGF-BB protein in conditioned medium compared to controls of ARPE-19 cells. In addition, two different early passage cultures of RPE showed increased PDGF-BB protein after pentosidine treatment compared to unstimulated controls. The enhanced production of PDGF-BB could play a role in the maintenance of the RPB-Bruch's membrane complex and influence changes associated with diabetes and aging.

Original languageEnglish (US)
Pages (from-to)411-419
Number of pages9
JournalExperimental Eye Research
Volume66
Issue number4
DOIs
StatePublished - Apr 1998

Keywords

  • Advanced glycation endproduct (AGE)
  • Nonenzymatic glycation
  • Pentosidine
  • Platelet-derived growth factor (PDGF)
  • Retinal pigment epithelium (RPE)

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

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