The 17-gene ethanolamine (eut) operon of Salmonella typhimurium encodes five homologues of carboxysome shell proteins

Eric Kofoid, Chad Rappleye, Igor Stojiljkovic, John Roth

Research output: Contribution to journalArticle

184 Citations (Scopus)

Abstract

The eut operon of Salmonella typhimurium encodes proteins involved in the cobalamin-dependent degradation of ethanolamine. Previous genetic analysis revealed six eut genes that are needed for aerobic use of ethanolamine; one (eutR), encodes a positive regulator which mediates induction of the operon by vitamin B12 plus ethanolamine. The DNA sequence of the eut operon included 17 genes, suggesting a more complex pathway than that revealed genetically. We have correlated an open reading frame in the sequence with each of the previously identified genes. Nonpolar insertion and deletion mutations made with the Tn10-derived transposable element T-POP showed that at least 10 of the 11 previously undetected eut genes have no Eut phenotype under the conditions tested. Of the dispensable eut genes, five encode apparent homologues of proteins that serve (in other organisms) as shell proteins of the carboxysome. This bacterial organelle, found in photosynthetic and sulfur-oxidizing bacteria, may contribute to CO2 fixation by concentrating CO2 and excluding oxygen. The presence of these homologues in the eut operon of Salmonella suggests that CO2 fixation may be a feature of ethanolamine catabolism in Salmonella.

Original languageEnglish (US)
Pages (from-to)5317-5329
Number of pages13
JournalJournal of Bacteriology
Volume181
Issue number17
StatePublished - Sep 1999
Externally publishedYes

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Ethanolamine
Salmonella typhimurium
Operon
Genes
Vitamin B 12
Proteins
Salmonella
INDEL Mutation
DNA Transposable Elements
Sulfur
Organelles
Open Reading Frames
Oxygen
Bacteria
Phenotype

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

The 17-gene ethanolamine (eut) operon of Salmonella typhimurium encodes five homologues of carboxysome shell proteins. / Kofoid, Eric; Rappleye, Chad; Stojiljkovic, Igor; Roth, John.

In: Journal of Bacteriology, Vol. 181, No. 17, 09.1999, p. 5317-5329.

Research output: Contribution to journalArticle

Kofoid, E, Rappleye, C, Stojiljkovic, I & Roth, J 1999, 'The 17-gene ethanolamine (eut) operon of Salmonella typhimurium encodes five homologues of carboxysome shell proteins', Journal of Bacteriology, vol. 181, no. 17, pp. 5317-5329.
Kofoid, Eric ; Rappleye, Chad ; Stojiljkovic, Igor ; Roth, John. / The 17-gene ethanolamine (eut) operon of Salmonella typhimurium encodes five homologues of carboxysome shell proteins. In: Journal of Bacteriology. 1999 ; Vol. 181, No. 17. pp. 5317-5329.
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