Tetratrichomonas spp. and Pentatrichomonas hominis are not persistently detectable after intravaginal inoculation of estrous heifers

Eduardo R. Cobo, Lynette B. Corbeil, Dalen W. Agnew, Karen VanHoosear, Alexander Friend, Dorthe R. Olesen, Robert Bondurant

Research output: Contribution to journalArticle

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Abstract

Virgin heifers (44) were intravaginally inoculated at estrus with low (106) or high (108) doses of live Tetratrichomonas sp., Pentatrichomonas hominis (P. hominis), or Tritrichomonas foetus (T. foetus). Controls were inoculated with Diamond's trypticase yeast extract maltose media. Genital infection was determined by culture of cervico-vaginal mucus (CVM) in Schneider's media and InPouch TF® as well as by polymerase chain reaction/restriction fragment length polymorphism (PCR/RFLP). The presence of trichomonads in fecal samples was determined by culture in Schneider's medium and PCR/RFLP. In CVM samples, tetratrichomonads were found by PCR/RFLP and Schneider's culture only sporadically at intermittent weeks. The presence of tetratrichomonads was not associated with the dose in the experimental vaginal inoculation since Tetratrichomonas sp. appeared more frequently in heifers inoculated with a low dose of tetratrichomonads than in heifers inoculated with a high dose of tetratrichomonads. Moreover, Tetratrichomonas spp. were isolated not only in heifers inoculated with tetratrichomonads but also in control heifers and in heifers inoculated with P. hominis. In feces, Tetratrichomonas spp. were frequently identified by culture in Schneider's and by PCR/RFLP in heifers of all groups. P. hominis was never found in CVM or feces by any method. Based on the common appearance of tetratrichomonads in feces and vaginal secretions, it appears that tetratrichomonads were detected periodically in the vagina of heifers as a consequence of repeated contamination from feces and not as a result of experimental infection. In summary, in this study, the strains of Tetratrichomonas sp. and P. hominis did not establish persistent infection in heifers.

Original languageEnglish (US)
Pages (from-to)18-26
Number of pages9
JournalVeterinary Parasitology
Volume150
Issue number1-2
DOIs
StatePublished - Nov 30 2007

Fingerprint

Pentatrichomonas hominis
Tetratrichomonas
Feces
Restriction Fragment Length Polymorphisms
heifers
Mucus
Polymerase Chain Reaction
Tritrichomonas foetus
Infection
restriction fragment length polymorphism
feces
polymerase chain reaction
mucus
Diamond
Maltose
Estrus
Vagina
dosage
Yeasts
infection

Keywords

  • Bovine
  • Fecal contamination
  • Genital tract
  • Pentatrichomonas hominis
  • Tetratrichomonads
  • Tritrichomonas foetus

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)

Cite this

Tetratrichomonas spp. and Pentatrichomonas hominis are not persistently detectable after intravaginal inoculation of estrous heifers. / Cobo, Eduardo R.; Corbeil, Lynette B.; Agnew, Dalen W.; VanHoosear, Karen; Friend, Alexander; Olesen, Dorthe R.; Bondurant, Robert.

In: Veterinary Parasitology, Vol. 150, No. 1-2, 30.11.2007, p. 18-26.

Research output: Contribution to journalArticle

Cobo, Eduardo R. ; Corbeil, Lynette B. ; Agnew, Dalen W. ; VanHoosear, Karen ; Friend, Alexander ; Olesen, Dorthe R. ; Bondurant, Robert. / Tetratrichomonas spp. and Pentatrichomonas hominis are not persistently detectable after intravaginal inoculation of estrous heifers. In: Veterinary Parasitology. 2007 ; Vol. 150, No. 1-2. pp. 18-26.
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AU - Cobo, Eduardo R.

AU - Corbeil, Lynette B.

AU - Agnew, Dalen W.

AU - VanHoosear, Karen

AU - Friend, Alexander

AU - Olesen, Dorthe R.

AU - Bondurant, Robert

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