Tetrahymena RIB72A and RIB72B are microtubule inner proteins in the ciliary doublet microtubules

Daniel Stoddard, Ying Zhao, Brian A. Bayless, Long Gui, Panagiota Louka, Drashti Dave, Swati Suryawanshi, Raphaël F.X. Tomasi, Pascale Dupuis-Williams, Charles N. Baroud, Jacek Gaertig, Mark Winey, Daniela Nicastro

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Doublet and triplet microtubules are essential and highly stable core structures of centrioles, basal bodies, cilia, and flagella. In contrast to dynamic cytoplasmic microtubules, their luminal surface is coated with regularly arranged microtubule inner proteins (MIPs). However, the protein composition and biological function(s) of MIPs remain poorly under-stood. Using genetic, biochemical, and imaging techniques, we identified Tetrahymena RIB72A and RIB72B proteins as ciliary MIPs. Fluorescence imaging of tagged RIB72A and RIB72B showed that both proteins colocalize to Tetrahymena cilia and basal bodies but assemble independently. Cryoelectron tomography of RIB72A and/or RIB72B knockout strains revealed major structural defects in the ciliary A-tubule involving MIP1, MIP4, and MIP6 structures. The defects of individual mutants were complementary in the double mutant. All mutants had reduced swimming speed and ciliary beat frequencies, and high-speed video imaging revealed abnormal highly curved cilia during power stroke. Our results show that RIB72A and RIB72B are crucial for the structural assembly of ciliary A-tubule MIPs and are important for proper ciliary motility.

Original languageEnglish (US)
Pages (from-to)2566-2577
Number of pages12
JournalMolecular Biology of the Cell
Volume29
Issue number21
DOIs
StatePublished - Oct 15 2018

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Microtubule Proteins
Tetrahymena
Microtubules
Cilia
Basal Bodies
Centrioles
Proteins
Flagella
Optical Imaging
Molecular Biology
Stroke
Tomography

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Stoddard, D., Zhao, Y., Bayless, B. A., Gui, L., Louka, P., Dave, D., ... Nicastro, D. (2018). Tetrahymena RIB72A and RIB72B are microtubule inner proteins in the ciliary doublet microtubules. Molecular Biology of the Cell, 29(21), 2566-2577. https://doi.org/10.1091/mbc.E18-06-0405

Tetrahymena RIB72A and RIB72B are microtubule inner proteins in the ciliary doublet microtubules. / Stoddard, Daniel; Zhao, Ying; Bayless, Brian A.; Gui, Long; Louka, Panagiota; Dave, Drashti; Suryawanshi, Swati; Tomasi, Raphaël F.X.; Dupuis-Williams, Pascale; Baroud, Charles N.; Gaertig, Jacek; Winey, Mark; Nicastro, Daniela.

In: Molecular Biology of the Cell, Vol. 29, No. 21, 15.10.2018, p. 2566-2577.

Research output: Contribution to journalArticle

Stoddard, D, Zhao, Y, Bayless, BA, Gui, L, Louka, P, Dave, D, Suryawanshi, S, Tomasi, RFX, Dupuis-Williams, P, Baroud, CN, Gaertig, J, Winey, M & Nicastro, D 2018, 'Tetrahymena RIB72A and RIB72B are microtubule inner proteins in the ciliary doublet microtubules', Molecular Biology of the Cell, vol. 29, no. 21, pp. 2566-2577. https://doi.org/10.1091/mbc.E18-06-0405
Stoddard, Daniel ; Zhao, Ying ; Bayless, Brian A. ; Gui, Long ; Louka, Panagiota ; Dave, Drashti ; Suryawanshi, Swati ; Tomasi, Raphaël F.X. ; Dupuis-Williams, Pascale ; Baroud, Charles N. ; Gaertig, Jacek ; Winey, Mark ; Nicastro, Daniela. / Tetrahymena RIB72A and RIB72B are microtubule inner proteins in the ciliary doublet microtubules. In: Molecular Biology of the Cell. 2018 ; Vol. 29, No. 21. pp. 2566-2577.
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