Reported is the mechanism of β2-integrin activation and increase in adhesion efficiency following neutrophil tethering to either E-selectin or L-selectin. Adhesion efficiency is defined as the fraction of total cell collisions that result in stable adhesion, and is computed by fitting the adhesion kinetics data to two-body collision theory. Independent of chemotactic stimulation, neutrophil tethering via E-selectin, and not L-selectin or PSGL1, induced a two-fold increase in β2-integrin expression and a four-fold boost in adhesion efficiency. Activation appeared to be mechanically transduced during selectin tethering as indicated by a distinct shear dependence on phosphorylation and signaling through p38 MAP kinase over a narrow range of applied shear rates.
|Original language||English (US)|
|Journal||Annals of Biomedical Engineering|
|Issue number||SUPPL. 1|
|Publication status||Published - 2000|
ASJC Scopus subject areas
- Biomedical Engineering