Tethering through selectins signals neutrophil adhesion in sheared suspension

Daniel Mcdonough, Eric Hentzen, Rupa Rao, C. Wayne Smith, Scott Simon

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Abstract

Reported is the mechanism of β2-integrin activation and increase in adhesion efficiency following neutrophil tethering to either E-selectin or L-selectin. Adhesion efficiency is defined as the fraction of total cell collisions that result in stable adhesion, and is computed by fitting the adhesion kinetics data to two-body collision theory. Independent of chemotactic stimulation, neutrophil tethering via E-selectin, and not L-selectin or PSGL1, induced a two-fold increase in β2-integrin expression and a four-fold boost in adhesion efficiency. Activation appeared to be mechanically transduced during selectin tethering as indicated by a distinct shear dependence on phosphorylation and signaling through p38 MAP kinase over a narrow range of applied shear rates.

Original languageEnglish (US)
JournalAnnals of Biomedical Engineering
Volume28
Issue numberSUPPL. 1
Publication statusPublished - 2000

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ASJC Scopus subject areas

  • Biomedical Engineering

Cite this

Mcdonough, D., Hentzen, E., Rao, R., Smith, C. W., & Simon, S. (2000). Tethering through selectins signals neutrophil adhesion in sheared suspension. Annals of Biomedical Engineering, 28(SUPPL. 1).