Testis and somatic Xrcc-1 DNA repair gene expression

Christi A. Walter, Jianwei Lu, Mukesh Bhakta, Zi Qiang Zhou, Larry H. Thompson, John R. McCarrey

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The human XRCC1 gene has been shown to be involved in DNA strand-break repair using the Chinese hamster ovary cell mutant EM9. The purpose of this study was to characterize the expression of Xrcc-1 to determine if there is tissue-specific expression and to provide a baseline of information for future studies that may involve altering Xrcc-1 expression in mice. Normal young adult male testis and enriched populations of pachytene spermatocytes and round spermatids displayed significantly higher levels of Xrcc-1 expression than other mouse tissue, although Xrcc-1 transcripts were found in low abundance in all tested tissues. Cultured mouse cell lines displayed levels of expression similar to male germ cells, which is a striking contrast to the levels of expression obtained in somatic tissues from the mouse. The relatively high levels of expression identified in male germ cells indicate Xrcc-1 may have an important role in male germ cell physiology.

Original languageEnglish (US)
Pages (from-to)451-461
Number of pages11
JournalSomatic Cell and Molecular Genetics
Volume20
Issue number6
DOIs
StatePublished - Nov 1994
Externally publishedYes

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DNA Repair
Testis
Germ Cells
Gene Expression
Cell Physiological Phenomena
DNA Breaks
Spermatocytes
Spermatids
Cricetulus
Young Adult
Ovary
Cultured Cells
Cell Line
Population
Genes

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Walter, C. A., Lu, J., Bhakta, M., Zhou, Z. Q., Thompson, L. H., & McCarrey, J. R. (1994). Testis and somatic Xrcc-1 DNA repair gene expression. Somatic Cell and Molecular Genetics, 20(6), 451-461. https://doi.org/10.1007/BF02255837

Testis and somatic Xrcc-1 DNA repair gene expression. / Walter, Christi A.; Lu, Jianwei; Bhakta, Mukesh; Zhou, Zi Qiang; Thompson, Larry H.; McCarrey, John R.

In: Somatic Cell and Molecular Genetics, Vol. 20, No. 6, 11.1994, p. 451-461.

Research output: Contribution to journalArticle

Walter, CA, Lu, J, Bhakta, M, Zhou, ZQ, Thompson, LH & McCarrey, JR 1994, 'Testis and somatic Xrcc-1 DNA repair gene expression', Somatic Cell and Molecular Genetics, vol. 20, no. 6, pp. 451-461. https://doi.org/10.1007/BF02255837
Walter CA, Lu J, Bhakta M, Zhou ZQ, Thompson LH, McCarrey JR. Testis and somatic Xrcc-1 DNA repair gene expression. Somatic Cell and Molecular Genetics. 1994 Nov;20(6):451-461. https://doi.org/10.1007/BF02255837
Walter, Christi A. ; Lu, Jianwei ; Bhakta, Mukesh ; Zhou, Zi Qiang ; Thompson, Larry H. ; McCarrey, John R. / Testis and somatic Xrcc-1 DNA repair gene expression. In: Somatic Cell and Molecular Genetics. 1994 ; Vol. 20, No. 6. pp. 451-461.
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