Test of a two-pathway model for small-solute exchange across the capillary wall

B. M. Fu, R. H. Adamson, F. E. Curry

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

We previously proposed a two-pathway model for solute and water transport across vascular endothelium (Fu, B. M., R. Tsay, F. E. Curry, and S. Weinbaum. J. Biomech. Eng. 116: 502-513, 1994) that hypothesized the existence of a continuous slit 2 nm wide along tight junction strands within the interendothelial cleft in parallel with 20 x 150-nm breaks in tight junctions. We tested this model by measuring capillary permeability coefficients (P) to a small solute (sodium fluorescein, radius 0.45 nm), assumed to permeate primarily the 2-nm small pore, and an intermediate-sized solute (FITC-α-lactalbumin, radius 2.01 nm) excluded from the small pore. Mean values of the paired diffusive permeability coefficients, P(sodium fluorescein) and p(FITC-α-lactalbumin), were 34.4 and 2.9 x 10-6 cm/s, respectively, after corrections for solvent drag and free dye (n = 26). These permeabilities were accounted for by transport through the large-break pathway without the additional capacity of the hypothetical 2-nm pathway. As a further test we examined the relative reductions of P(sodium fluorescein) and p(FITC-α-lactalbumin) produced by elevated intracellular cAMP. Within 20 min after the introduction of rolipram and forskolin, P(sodium fluorescein) and P(FITC-α-lactalbulmin) decreased to 0.67 and 0.64 times their respective baseline values. These similar responses to permeability decrease were evidence that the two solutes were carried by a common pathway. Combined results in both control and reduced permeability states did not support the hypothesis that a separate pathway across tight junctions is available for solutes with a radius as large as 0.75 nm. If such a pathway is present, then its size must be smaller than that of sodium fluorescein.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume274
Issue number6 43-6
StatePublished - 1998

Fingerprint

Fluorescein
Fluorescein-5-isothiocyanate
Lactalbumin
Tight Junctions
Permeability
Rolipram
Vascular Endothelium
Capillary Permeability
Colforsin
Coloring Agents
Water

Keywords

  • Forskolin
  • Paired measurements on single capillaries
  • Quantitative fluorescence microscope photometry
  • Rolipram
  • Three-dimensional junctionpore matrix model for interendothelial cleft

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Test of a two-pathway model for small-solute exchange across the capillary wall. / Fu, B. M.; Adamson, R. H.; Curry, F. E.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 274, No. 6 43-6, 1998.

Research output: Contribution to journalArticle

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